Skip to main content
Log in

Use of the FLOTAC technique to diagnosing parasites of the urinary tract of dogs

  • Short Communication
  • Published:
Parasitology Research Aims and scope Submit manuscript

Abstract

Among the nematodes that infect the urinary tract of dogs, the Dioctophyma renale and Capillaria plica are those more frequently reported. For a long time, sedimentation was the only method used to detect eggs of these parasites in urine. The aim of this study was to analyze urine samples (n = 54) of dogs, obtained by bladder catheterization or cystocentesis, by using a modified FLOTAC technique. Animals were divided into two groups, i.e., with (n = 20) and without (n = 34) suspicion of urinary disease. The overall positivity herein observed was 3.8 % (2/54), being all animals (10 %; 2/20) from the group with suspicion of urinary disease. In the first positive sample, a single egg of D. renale was detected, whereas in the second sample two trematode-like eggs were observed. This is the first short survey employed to detect eggs of parasites that inhabit the urinary tract of dogs using a modified FLOTAC technique; in addition, for the first time, eggs of D. renale have been detected using this tool.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1

References

  • Anderson RC (1992) Nematode parasites of vertebrates - their development and transmission, Cambridge, pp 53–539

  • Baker DC, Campbell TW, Denicola D, Fettman MJ, Lassen ED, Rebar A, Weiser G (2006) Haematology and Biochemistry Veterinary Clinic, São Paulo., p 532

    Google Scholar 

  • Bassoa W, Spänhauerb Z, Deplazesa P (2014) Capillaria plica (syn. Pearsonema plica) infection in a dog with chronic pollakiuria: Challenges in the diagnosis and treatment. Parasitol Int 63:140–142

    Article  Google Scholar 

  • Callegari D, Kramer L, Cantoni AM, Di Lecce R, Dodi PL, Grandi G (2010) Canine bexiga (Capillaria plica) infecção associada com amiloidose glomerular. Vet Parasitol 168:338–341

    Article  CAS  PubMed  Google Scholar 

  • Callens AJ, Bartges JW (2015) Urinalysis. Vet Clin Nort Am Small Anim Pract 45:621–637

    Article  Google Scholar 

  • Coppo JA, Brem JJ (1983) Canine dioctophymosis in the North East of Argentine. J São Paulo Inst Trop Med 25:259–262

    CAS  Google Scholar 

  • Coppola MG, Petrullo L, Gualdieri L, Morgoglione ME, Maurelli MP, Rinaldi L, Musella V, Alfano S, Piccoli S, Cringoli G, Atripaldi L, Rossano F (2009) Abstract Livro de XXXVIII Congresso Nazionale AMCLI. AMCLI Milano, Rimini (Itália), p 103

    Google Scholar 

  • Cringoli G, Rinaldi L, Maurelli MP, Utzinger J (2010) FLOTAC: new multivalente 175 techniques for qualitative and quantitative copromicroscopic diagnosis of parasites in animals and humans. Nat Protoc 5:503–515

    Article  CAS  PubMed  Google Scholar 

  • Easwaran KR, Ravindran R, Pillai KM (2003) Parasitic infection of some wild animals at Thakkady in Kerala. Zoo’s Print J 18:1030

    Article  Google Scholar 

  • Emamapour SR, Borji H, Nagibi A (2015) An epidemiological survey on intestinal helminths of stray dogs in Mashhad, Northeast of Iran. J Parasit Dis 39:266–271

    Article  PubMed  Google Scholar 

  • Ferreira VL, Medeiros FP, July JR, Raso TF (2010) Dioctophyma renale in a dog: clinical diagnosis and surgical treatment. Vet Parasitol 168:151–155

    Article  PubMed  Google Scholar 

  • Herman JS, Chiodini PL (2009) Gnathostomiasis, another emerging imported disease. Clin Microbiol Rev 22:484–492

    Article  PubMed  PubMed Central  Google Scholar 

  • Inkelmann MA, Kommers GD, Trost ME, Barros CSL, Fighera RA, Irigoyen LF, Silveira I (2012) Lesions of the urinary system in 1,063 dogs. Braz J Vet Res 32:761–771

    Google Scholar 

  • Kano FS, Shimada MT, Suzuki SN, Osaki SC, Menarim BC, Ruthes FRV, Laidane Filho MA (2003) Dioctophymosis occurrence in two dogs in Guarapuava city—Parana State. Semina Ciênc Agrar 4:177–180

    Article  Google Scholar 

  • Lima VFS, Cringoli G, Rinaldi L, Monteiro MFM, Calado AMC, Ramos RAN, Meira-Santos PO, Alves LC (2015) A comparison of Mini-FLOTAC and FLOTAC with classic methods to diagnosing intestinal parasites of dogs from Brazil. Parasitol Res 144:3529–3533

    Article  Google Scholar 

  • Maurelli MP, Rinaldi L, Rubino G, Lia R, Mussela V, Cringoli G (2014) FLOTAC and Mini-FLOTAC for uro-microscopic diagnosis of Capillaria plica (syn. Pearsonema plica) in dogs. BMC Res Notes 2:591

    Article  Google Scholar 

  • Mesquita LR, Rahal SC, Faria LG, Takahira RK, Rocha NS, Mamprim MJ, Oliveira HS (2014) Pre- and post-operative evaluations of eight dogs following right nephrectomy due to Dioctophyma renale. Vet Q 4:167–171

    Article  Google Scholar 

  • Nakagawa TLR, Bracarense APRFL, Reis ACF, Yamamura MH, Headley SA (2007) Giant kidney worm (Dioctophyma renale) infections in dogs from Northern Paraná, Brazil. Vet Parasitol 145:366–370

    Article  PubMed  Google Scholar 

  • Nonaka N, Nakamura S, Inoue T, Oku Y, Katakura K, Matsumoto J, Mathis A, Chembesofu M, Phiri IGK (2011) Coprological survey of alimentary tract parasites in dogs from Zambia and evaluation of a coproantigen assay for canine echinococcosis. Ann Trop Med Parasitol 105:521–531

    CAS  PubMed  PubMed Central  Google Scholar 

  • Pereira BJ, Girardelli GL, Trivilin LO, Lima VR, Nunes LC, Martins IV (2006) The occurrence of dioctophymosis in dogs from Municipality of Cachoeiro do Itapemirim in the State of Espírito Santo, Brazil, from May to December of 2004. Braz J Vet Parasitol 15:123–125

    Google Scholar 

  • Ramos RAN, Lima VFS, Monteiro MFM, Santana MA, Lepold R, Faustino MAG, Rinaldi L, Cringoli G, Alves LC (2015) New insights into diagnosis of Platynosomum fastosum (Trematoda: Dicrocoeliidae) in cats. Parasitol Res.

  • Silveira LL, Lemos LS, Ferreira FS, Freitas MV, Pereira MAC, Carvalho CB (2009) Comparative analysis among the techniques of sedimentation-centrifugal and fast-sedimentation (Paratest®) on identification of eggs of Dioctophyma renale in piss of dogs. BCA J Bras Cie Anim 2:15–158

    Google Scholar 

  • Swenson CL, Boisvert AM, Kruger JM, Gibbons-Burgener SN (2004) Evaluation of modified Wright-staining of urine sediment as a method for accurate detection of bacteriuria in dogs. J Am Vet Med 224:1282–1289

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Victor Fernando Santana Lima.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Lima, V.F.S., Ramos, R.A.N., Lepold, R. et al. Use of the FLOTAC technique to diagnosing parasites of the urinary tract of dogs. Parasitol Res 115, 1737–1739 (2016). https://doi.org/10.1007/s00436-016-4950-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00436-016-4950-4

Keywords

Navigation