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Greek Pinus essential oils: larvicidal activity and repellency against Aedes albopictus (Diptera: Culicidae)

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Abstract

The needle volatiles metabolites of seven Pinus spp.: Pinus nigra (3 samples), Pinus stankewiczii, Pinus brutia, Pinus halepensis, Pinus canariensis, Pinus pinaster and Pinus strobus from Greece were determined by gas chromatography and gas chromatography–mass spectrometry. P. nigra and P. canariensis essential oils were dominated by α-pinene (24.9–28.9 % and 15 %, respectively) and germacrene D (20.3–31.9 % and 55.8 %, respectively), whereas P. brutia and P. strobus by α-pinene (20.6 % and 31.4 %, respectively) and β-pinene (31.7 % and 33.6 %, respectively). P. halepensis and P. pinaster oils were characterized by β-caryophyllene (28.5 % and 22.5 %, respectively). Finally, β-pinene (31.4 %), germacrene D (23.3 %) and α-pinene (17.5 %) were the most abundant compounds in the needle oil of P. stankewiczii. Additionally the larvicidal and repellent properties of their essential oils were evaluated against Aedes albopictus, a mosquito of great ecological and medical importance. The results of bioassays revealed that repellent abilities of the tested essential oils were more potent than their larvicidal activities. The essential oils of P. brutia, P. halepensis and P. stankewiczii presented considerable larvicidal activity (LC50 values 67.04 mgL−1 and 70.21 mgL−1, respectively), while the others were weak to inactive against larvae. The essential oils of P. halepensis, P. brutia, and P. stankewiczii presented a high repellent activity, even at the dose of 0.2 μL cm−2, while in the dose of 0.4 μL cm−2, almost all the tested EOs displayed protection against the mosquito.

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Acknowledgments

The authors would like to thank Prof. B. Galatis (J. & A. N. Diomedes Botanic Garden, University of Athens) for providing the plant material and Dr. I. Vallianatou (J. & A. N. Diomedes Botanic Garden, University of Athens) for the collection and plant identification.

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Authors and Affiliations

  1. Department of Pharmacognosy and Chemistry of Natural Products, School of Pharmacy, University of Athens, Panepistimioupoli Zographou, 157 71, Athens, Greece

    Katerina Koutsaviti, Danae Pitarokili & Olga Tzakou

  2. Laboratory of Biological Control of Pesticides, Benaki Phytopathological Institute, 8 S. Delta Str., 145 61, Kifissia, Athens, Greece

    Athanassios Giatropoulos

  3. Laboratory of Agricultural Zoology and Entomology, Agricultural University of Athens, 75 Iera Odos Str., 118 55, Athens, Greece

    Athanassios Giatropoulos

  4. Laboratory of Agricultural Entomology, Department of Entomology and Agricultural Zoology, Benaki Phytopathological Institute, 8 S. Delta Str., 145 61, Kifissia, Athens, Greece

    Dimitrios Papachristos & Antonios Michaelakis

Authors
  1. Katerina Koutsaviti
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  2. Athanassios Giatropoulos
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  3. Danae Pitarokili
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  4. Dimitrios Papachristos
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  5. Antonios Michaelakis
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  6. Olga Tzakou
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Correspondence to Antonios Michaelakis or Olga Tzakou.

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Koutsaviti, K., Giatropoulos, A., Pitarokili, D. et al. Greek Pinus essential oils: larvicidal activity and repellency against Aedes albopictus (Diptera: Culicidae). Parasitol Res 114, 583–592 (2015). https://doi.org/10.1007/s00436-014-4220-2

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