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Ultrastructural characterization of male and female Physaloptera rara (Spirurida: Physalopteridae): feline stomach worms

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Abstract

Physaloptera rara (Spirurida: Physalopteridae) has been found in dogs, coyotes, raccoons, wolves, foxes, cats, and bobcats in North America. The parasites’ developmental cycles involve insects, including beetles, cockroaches, and crickets, as intermediate hosts. The nematodes firmly attach to the wall of the stomach and duodenum, where they feed on the mucosa and suck blood. Frequent movement of these nematodes results in erosions and ulcers in the gastrointestinal tract. The present study reports the morphological features of adult P. rara using scanning electron microscopy. Adult worms were removed from the stomach of an infected domestic cat. Male and female worms measured 25–29 and 27–41 mm, respectively. The worms were stout and the cuticle was reflected over the lips to form a large cephalic collarette with fine transverse striations. The worms possessed two large, simple triangular lateral pseudolabia, each armed with one external tooth, three internal teeth, two submedian cephalic papillae, an amphid, and three porous-like circumscribed regions. The internal margins of the lips had a pair of cuticular folds. At the anterior end of both male and female worms, an excretory pore was located on the ventral side and a pair of lateral ciliated cervical papillae was seen. The vulva was anterior to the middle of the body of female worms. The tail ends of the female worms were stumpy, with two large phasmids near their extremities. The males’ tails bore large lateral alae. Ventral ornamentation, in male worms, was composed of three different cuticular patterns; coblestone-like formations, longitudinal cuticular ridges, and rows of bead-like structures. The spicules were unequal and dissimilar; the right spicule had a thick end and the left spicule had a sharp tip. At the posterior end of the males, four pairs of stalked precloacal papillae, three pairs of postcloacal papillae, and two phasmids were present. Three and four sessile papillae were seen directly anterior and posterior to the cloaca, respectively. The middle papilla of the three sessile papillae, directly anterior to cloaca was different in shape and size.

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Acknowledgments

The authors extend their appreciation to Professor Larry A. Arsenaul, Head of the Electron Microscope Facility, Department of Pathology and Molecular Medicine, McMaster University, Canada, for his assistance in providing all facilities during the study. Also, the valuable help of Mr. Ernie Spitzer, Chief Technician, and all technicians in the electron microscope facility is acknowledged. Special thanks are expressed to Christine M. Budke, Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, USA, for her constructive comments which improved the manuscript.

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  1. Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

    Soraya Naem & Reza Asadi

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  1. Soraya Naem
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  2. Reza Asadi
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Correspondence to Soraya Naem.

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Naem, S., Asadi, R. Ultrastructural characterization of male and female Physaloptera rara (Spirurida: Physalopteridae): feline stomach worms. Parasitol Res 112, 1983–1990 (2013). https://doi.org/10.1007/s00436-013-3356-9

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