Abstract
Cystic echinococcosis (CE) is a zoonotic disease caused by several members of the Echinococcus granulosus species complex. In East Africa, several species/strains are known to occur in livestock and humans, but host preferences, relative frequencies and spatial distribution of these taxa are poorly known. Here, we contribute livestock data for Maasailand of southern Kenya. Total CE prevalence was 25.8 % in cattle (151/587), 16.5 % in sheep (71/430) and 10.8 % in goats (21/194), which is a significant increase compared to surveys done about three decades ago. The majority of cysts occurred in the liver (56 % in cattle, 70 % in sheep and 65 % in goats). Molecular characterization by PCR–RFLP and sequencing of parts of the mitochondrial nad-1 gene was done for a subsample of 285 cysts. E. granulosus G1 was dominant in all host species (200 of 201 cysts from cattle, 68 of 69 from sheep and 11 of 15 from goats); the remaining taxa were Echinococcus canadensis G6 (one cyst from sheep, four from goats) and Echinococcus ortleppi (one cyst from cattle). Considering cyst fertility, sheep appear to be the most important hosts for E. granulosus G1, while goats were found to be suitable hosts for E. canadensis G6 (three of four cysts were fertile). For the first time, E. ortleppi was found in cattle from southern Kenya. Our data show an intense and possibly increasing level of CE transmission in southern Kenya, and the predominance of E. granulosus G1, which appears to be particularly pathogenic to humans, calls for urgent control measures.
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References
Abdul J, Myadagsuren N, Matthew JN, Jex AR, Campbell BE, Gasser RB (2010) A first insight into the genotypes of Echinococcus granulosus from humans in Mongolia. Mol Cell Probes 25(1):49–54
Berhe G (2009) Abattoir survey on cattle hydatidosis in Tigray Region of Ethiopia. Trop Anim Health Prod 41(7):1347–1352
Casulli A, Zeyhle E, Brunetti E, Pozio E, Meroni V, Genco F, Filice C (2010) Molecular evidence of the camel strain (G6 genotype) of Echinococcus granulosus in humans from Turkana, Kenya. Trans R Soc Trop Med Hyg 104(1):29–32
Dinkel A, Njoroge EM, Zimmermann A, Walz M, Zeyhle E, Elmahdi IE, Mackenstedt U, Romig T (2004) A PCR system for detection of species and genotypes of the Echinococcus granulosus complex, with reference to the epidemiological situation in Eastern Africa. Int J Parasitol 34(5):645–653
Eckert J, Deplazes P, Craig, PS, Gemmell, MA, Gottstein B, Heath D, Jenkins DJ, Kamiya M, Lightowlers M (2001) Echinococcosis in animals: clinical aspects, diagnosis and treatment. In: Eckert J, Gemmel MA, Meslin FX, Pawlowski ZS (eds) World Health Organisation/World Organisation for Animal Health manual on echinococcosis in humans and animals: a public health problem of global concern. Paris, France, pp 72–99
Evangelou P (1984) Livestock development in Kenya's Maasailand: pastoralists transition to a market economy. Westview. 5500 Central Avenue, Boulder, Colorado, USA
Hüttner M, Nakao M, Wassermann T, Siefert L, Boomker JDF, Dinkel A, Sako Y, Mackenstedt U, Romig T, Ito A (2008) Genetic characterization and phylogenetic position of Echinococcus felidis Ortlepp, 1937 (Cestoda: Taeniidae) from the African lion. Int J Parasitol 38(7):861–868
Hüttner M, Siefert L, Mackenstedt U, Romig T (2009) A survey of Echinococcus species in wild carnivores and livestock in East Africa. Int J Parasitol 39(11):1269–1276
Ibrahim K, Romig T, Kern P, Omer RA (2011) A molecular survey on cystic echinococcosis in Sinnar area, Blue Nile state (Sudan). Chin Med J 124(18):2829–2833
Kaplan I, Dobert MK, Marvin BJ, McLaughlin JL, Whitaker DP (1976) Area handbook for Kenya, 2nd edn. Foreign area studies, DA Pam 550–56, Washington DC, USA
Macpherson CNL (1985) Epidemiology of hydatid disease in Kenya: a study of the domestic intermediate hosts in Masailand. Trans R Soc Trop Med Hyg 79(2):209–217
Macpherson CNL, Craig PS, Romig T, Zeyhle E, Watschinger H (1989) Observations on human echinococcosis (hydatidosis) and evaluation of transmission factors in the Maasai of northern Tanzania. Ann Trop Med Parasitol 83(5):489–497
Magambo J, Njoroge E, Zeyhle E (2006) Epidemiology and control of echinococcosis in sub-Saharan Africa. Parasitol Int 55(suppl):193–195
Maillard S, Benchikh-Elfegoun MC, Knapp J, Bart JM, Koskei P, Gottstein B, Piarroux R (2007) Taxonomic position and geographical distribution of the common sheep G1 and camel G6 strains of Echinococcus granulosus in three African countries. Parasitol Res 100(3):495–503
Maillard S, Gottstein B, Haag KL, Ma S, Colovic I, Benchikh-Elfegoun MC, Knapp J, Piarroux R (2009) The Emsb tandemly repeated multilocus microsatellite: a new tool to investigate genetic diversity of Echinococcus granulosus. J Clin Microbiol 47(11):3608–3616
Mulinge E, Magambo J, Mbae C, Hüttner M, Zeyhle E, Kern P, Romig T (2011) First report of Echinococcus ortleppi in Kenyan cattle. XXIVth World congress of hydatidology, Urumqi, China, 14–18 September 2011; Abstracts Book, pp 178.
Nakao M, Sako Y, Ito A (2003) Isolation of polymorphic microsatellite loci from the tapeworm Echinococcus multilocularis. Infect Genet Evol 3(3):159–163
Nakao M, McManus DP, Schantz PM, Craig PS, Ito A (2007) A molecular phylogeny of the genus Echinococcus inferred from complete mitochondrial genomes. Parasitology 134(5):713–722
Njoroge EM, Mbithi PM, Gathuma JM, Wachira TM, Magambo JK, Zeyhle E (2002) A study of cystic echinococcosis in slaughter animals in three selected areas of northern Turkana, Kenya. Vet Parasitol 104(1):85–91
Omer RA, Dinkel A, Romig T, Mackenstedt U, Elnahas AA, Ahmed ME, Elmalik KH, Adam A, Aradaib IE (2010) A molecular survey of cystic echinococcosis in Sudan. Vet Parasitol 169(11):340–346
Romig T, Omer RA, Zeyhle E, Hüttner M, Dinkel A, Siefert L, Elmahdi IE, Magambo J, Ocaido M, Menezes CN, Ahmed ME, Mbae C, Grobusch MP, Kern P (2011) Echinococcosis in sub-Saharan Africa: emerging complexity. Vet Parasitol 181(1):43–47
Thompson RCA, McManus DP (2001) Aetiology: parasites and lifecycles. In: Eckert J, Gemmel MA, Meslin FX, Pawlowski ZS (eds) World Health Organisation/World Organisation for Animal Health manual on echinococcosis in humans and animals: a public health problem of global concern. Paris, France, pp 1–19
Varcasia A, Canu S, Lightowlers AMW, Scala A, Garippa G (2006) Molecular characterization of Echinococcus granulosus strains in Sardinia. Parasitol Res 98(3):273–277
Varcasia A, Canu S, Kogkos A, Pipia AP, Scala A, Garippa G, Seimenis A (2007) Molecular characterization of Echinococcus granulosus in sheep and goats of Peloponnesus, Greece. Parasitol Res 101(4):1135–1139
Wachira TM, Bowles J, Zeyhle E, McManus DP (1993) Molecular examination of the sympatry and distribution of sheep and camel strains of Echinococcus granulosus in Kenya. AmJTrop Med Hyg 48(4):473–479
Acknowledgments
The study was financially supported by the German Academic Exchange Service DAAD (A/11/07888 scholarship) and Deutsche Forschungsgemeinschsft DFG (RO 3753-1/1, KE 282/7-1).
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The study complies with the current laws of Kenya.
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The authors declare that they have no conflict of interests.
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Addy, F., Alakonya, A., Wamae, N. et al. Prevalence and diversity of cystic echinococcosis in livestock in Maasailand, Kenya. Parasitol Res 111, 2289–2294 (2012). https://doi.org/10.1007/s00436-012-3082-8
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DOI: https://doi.org/10.1007/s00436-012-3082-8