Abstract
The present study was aimed to detect urinary proteins excreted in kala-azar patients. Urinary proteins were isolated by ammonium sulfate precipitation and purified by Amicon ultra using 3 kDa cutoff membrane device. The proteins were resolved on 12 % sodium dodecyl sulfate polyacrylamide gel electrophoresis and blots were developed with the patients sera. The excretion of urinary proteins was differential in kala-azar patients. A total eight proteins of molecular weights 25, 28, 46, 54, 57, 60, 72, and 95 kDa were detected in the urine of visceral leishmaniasis (VL) patients. On blot, the 57 kDa protein was found to be host origin and characterized as human kininogen. All other proteins were leishmanial origin. Out of 50 urine samples analyzed, the kininogen was detected in 45 urine samples. Following treatment, this protein was not detectable in the urine samples of any patient. The appearance of kininogen in urine of VL patients offers a novel possibility for the development of diagnostic tool and a test of cure.
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References
Decramer S, Gonzalez de Peredo A, Breuil B, Mischak H, Monsarrat B, Bascands JL, Schanstra JP (2008) Urine in clinical proteomics. Mol Cell Proteomics 7:1850–1862
Hermann A, Braun A, Figueroa CD, Esterl WM, Fritz H, Rehbock J (1996) Expression and cellular localization of kininogens in the human kidney. Kidney Int 50:79–84
Islam MZ, Itoh M, Mirza R, Ahmed I, Ekram AR, Sarder AH, Shamsuzzaman SM, Hashiguchi Y, Kimura E (2004) Direct agglutination test with urine samples for the diagnosis of kala-azar. AmJTrop Med Hyg 70:78–82
Kabanda A, Vandercam B, Bernard A, Lauwerys R, van Ypersele de Strihou C (1996) Low molecular weight proteinuria in human immunodeficiency virus-infected patients. Am J Kidney Dis 27:803–808
Kumar V, Gour JK, Bajpai S, Mishra M, Singh RK (2011) Detection of urinary antigens and their seroreactivity with serum of patients in Leishmania donovani infection. Asian Pac J Trop Med 4:367–370
Mishra BB, Singh RK, Srivastava A, Tripathi VJ, Tiwari VK (2009) Fighting against leishmaniasis: search of alkaloids as future true potential anti-leishmanial agents. Mini Rev Med Chem 9:107–123
Ozerdem D, Eroglu F, Genc A, Demirkazik M, Koltas IS (2009) Comparison of microscopic examination, rK39, and PCR for kala-azar diagnosis in Turkey. Parasitol Res 106:197–200
Sarkari B, Chance M, Hommel M (2002) Antigenuria in kala-azar: detection and partial characterisation of a carbohydrate antigen. Acta Trop 82:339–348
Schaub NP, Jones KJ, Nyalwidhe JO, Cazares LH, Karbassi ID, Semmes OJ, Feliberti EC, Perry RR, Drake RR (2009) Serum proteomic biomarker discovery reflective of stage and obesity in breast cancer patients. J Am Coll Surg 208:270–280
Singh RK, Pandey HP, Sundar S (2006) Kala-azar (kala-azar): challenges ahead. Indian J Med Res 123:331–344
Sundar S, Benjamin B (2003) Diagnosis and treatment of Indian kala-azar. J Assoc Phys India 51:195–201
Sundar S, Kumar A (2005) Challenges in the management of kala-azar. Indian Pediatr 42:523–526
Sundar S, Singh RK, Bimal SK, Gidwani K, Mishra A, Maurya R, Singh SK, Manandhar KD, Boelaert M, Rai M (2007) Comparative evaluation of parasitology and serological tests in the diagnosis of kala-azar in India: a phase III diagnostic accuracy study. Trop Med Int Health 12:284–289
Umemura H, Togawa A, Sogawa K, Satoh M, Mogushi K, Nishimura M, Tanaka H, Takizawa H, Kodera Y, Nomura F (2011) Identification of high molecular weight kininogen fragement as a marker for early gastric cancer by serum proteome analysis. J Gastroenterol 46:577–585
Vaish M, Mehrotra S, Chakravarty J, Sundar S (2011) Noninvasive molecular diagnosis of human kala-azar. J Clin Microbiol 49:2003–2005
Xiao D, Meng FL, He LH, Gu YX, Zhang J (2011) Analysis of the urinary peptidome associated with Helicobacter pylori infection. World J Gastroenterol 17:618–624
Acknowledgments
Financial assistance (no. 27 (0183)/08/EMR-II) received from Council of Scientific and Industrial Research, New Delhi, India is greatly acknowledged. The authors VK and MM are thankful to CSIR and UGC, New Delhi, respectively, for their research fellowships.
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Kumar, V., Mishra, M., Rajput, S.K. et al. Detection and diagnostic applicability of human urinary kininogen in kala-azar patients. Parasitol Res 111, 1851–1855 (2012). https://doi.org/10.1007/s00436-012-2931-9
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DOI: https://doi.org/10.1007/s00436-012-2931-9