Abstract
Amoebic keratitis is difficult to treat without total efficacy in some patients because of cysts, which are less susceptible than trophozoites to the usual treatments. The aim of this study is to evaluate the in vitro amoebicidal activity of the methanolic extracts of Satureja cuneifolia and Melissa officinalis. In the presence of methanolic extracts (ranging from 1.0 to 32.0 mg/ml), numbers of the viable Acanthamoe castellanii trophozoites and cysts were decreased during the experimental process. Both extracts showed a time- and dose-dependent amoebicidal action on the trophozoites and cysts. Among the extracts tested, S. cuneifolia showed the strongest amoebicidal effect on the trophozoites and cysts. In the presence of 32 mg/ml extract, no viable trophozoites were observed within 24 h. At the same concentration value, the extract was found effective against the cysts at a rate of 46.3% within 72 h of the experimental process. At 16 mg/ml extract concentration, no viable trophozoites were also observed in the 24th hour of the experiment. At the end of the experimental process, 34.7% of the cysts were killed by the extract. M. officinalis showed moderate amoebicidal effect. At the concentration of 32 mg/ml, 44.3% and 30.0% of the trophozoites and cysts were killed by the extract, respectively. Results obtained from these concentration values were found statistically different in terms of their actions both on trophozoites and cysts (p < 0.05).
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Akin Polat Z, Tepe B, Vural A (2007a) In vitro amoebicidal activity of Thymus sipyleus subsp. sipyleus var. sipyleus on Acanthamoeba castellanii and its cytotoxic potential on corneal cells. Parasitol Res 101:1551–1555
Akin Polat Z, Vural A, Tepe B, Cetin A (2007b) In vitro amoebicidal activity of four Allium species on Acanthamoeba castellanii and their cytotoxic potentials on corneal cells. Parasitol Res 101:397–402
Akin Polat Z, Vural A, Ozan F, Tepe B, Ozcelik S, Cetin A (2008) In vitro effect of garlic (Allium sativum) extract on Acanthamoeba castellanii and its cytotoxic potential on corneal cells. J Ocular Pharm Ther 24:8–14
Arrieta J, Reyes B, Calzada F, Cedillo-Rivera R (2001) Amoebicidal and giardicidal compounds from the leaves of Zanthoxylum liebmannianun. Fitoter 72:295–297
Carter WW, Gompf SG, Toney JF, Greene JN, Cutolo EP (2004) Disseminated Acanthamoeba sinusitis in a patient with AIDS: a possible role for early antiretroviral therapy. AIDS Read 14:41–49
Chu DM, Miles H, Toney D, Ngyuen C, Marciano-Cabral F (1998) Amebicidal activity of plant extracts from Southeast Asia on Acanthamoeba spp. Parasitol Res 84:746–752
Degerli S, Berk S, Malatyali E, Tepe B (2011) Screening of the in vitro amoebicidal activities of Pastinaca armenea (Fisch. & C.A.Mey.) and Inula oculus-christi (L.) on Acanthamoeba castellanii cysts and trophozoites. Parasitol Res. doi:10.1007/s00436-011-2524-z
Driebe WT (2003) Present status of contact lens-induced corneal infections. Ophthalmol Clin North Am 16:485–494
Feingold JM, Abraham J, Bilgrami S, Ngo N, Visvesara GS, Edwards RL, Tutschka PJ (1998) Acanthamoeba meningoencephalitis following autologous peripheral stem cell transplantation. Bone Marrow Transplant 22:297–300
Garcia LS, Brucker DA (1993) Diagnostic medical parasitology, 2nd edn. American Society for Microbiology Press, Washington, p 601
Goze I, Dag S, Tepe B, Akin Polat Z (2009) In vitro amoebicidal activity of Salvia staminea and S. caespitosa on Acanthamoeba castellanii and their cytotoxic potentials on corneal cells. J Ocular Pharm Ther 25:293–298
Hargrave SL, McCulley JP, Husseini Z (1999) Results of a trial of combined propamidine isethionate and neomycin therapy for Acanthamoeba keratitis. Brolene Study Group. Ophthalmol 106:952–957
Hay J, Seal DV (1996) Acanthamoeba keratitis: risk factors and outcome. Br J Ophthalmol 80:773–774
Holzgrabe U, Bechthold A (1999) Paradigmenwechsel in der Entwicklung antiinfektiver Chemotherapeutika. Chemother J 8:69–78
Kayser O, Kiderlen A, Croft SL (2002) Natural products as potential antiparasitic drugs. Studies in Natural Product Research, Atta-Ur-Rahman (eds) 26:779–848
Kayser O, Kiderlen AF, Croft SL (2003) Natural products as antiparasitic drugs. Parasitol Res 90:55–62
Khan NA (2003) Pathogenesis of Acanthamoeba infections. Microb Pathol 34:277–285
Khan NA (2009) Acanthamoeba biology and pathogenesis. Caister Academic Press, UK, p 85
Khunkitti W, Lloyd D, Furr JR, Russell AD (1997) Aspects of the mechanisms of action of biguanides on trophozoites and cysts of Acanthamoeba castellanii. J Appl Microbiol 82:107–114
Kosrirukvongs P, Wanachiwanawin D, Visvesvara GS (1999) Treatment of Acanthamoeba keratitis with chlorhexidine. Ophthalmol 106:798–802
Kumar R, Lloyd D (2002) Recent advances in the treatment of Acanthamoeba keratitis. Clin Infect Dis 35:434–441
Lloyd D, Turner NA, Khunkitti W (2001) Encystation in Acanthamoeba castellanii: development of biocide resistance. J Eukar Microbiol 48:11–16
Malatyali E, Tepe B, Degerli S, Berk S, Akpulat HA (2011) In vitro amoebicidal activity of four Peucedanum species on Acanthamoeba castellanii cysts and trophozoites. Parasitol Res. doi:10.1007/s00436-011-2466-5
Marciano-Cabral F, Cabral GA (2003) Acanthamoeba spp. as agents of disease in humans. Clin Microbiol Rev 16:273–307
Marciano-Cabral F, Puffenbarger R, Cabral GA (2000) The increasing importance of Acanthamoeba infections. J Eukar Microbiol 47:29–36
Martinez AJ, Visvesvara GS (1997) Free-living, amphizoic and opportunistic amebas. Brain Pathol 7:583–598
Mattana A, Biancu G, Alberti L, Accardo A, Delogu G, Fiori PL, Cappuccinelli P (2004) In vitro evaluation of the effectiveness of the macrolide rokitamycin and chlorpromazine against Acanthamoeba castellanii. Antimic Agent Chemother 48:4520–4527
Mills R (2003) Microbial keratitis, what's the preferred initial therapy? View 1: corneal scraping and combination antibiotic therapy is indicated. Br J Ophthalmol 87:1167–1169
Osato MS, Robinson NM, Wilhelmus KR, Jones DB (1991) In vitro evaluation of antimicrobial compounds for cysticidal activity against Acanthamoeba. Rev Infect Dis 13(suppl 5):S431–S435
Phillipson JD, Wright CW (1991) Can ethnopharmacology contribute to the development of antimalarial agents? J Ethnopharm 32:155–165
Rayo Camacho Corona M, Croft SL, Phillipson JD (2000) Natural products as sources of antiprotozoal drugs. Cur Op Anti-Infect Investig Drug 2:47–62
Rosenberg AS, Morgan MB (2001) Disseminated acanthamoebiasis presenting as lobular panniculitis with necrotizing vasculitis in a patient with AIDS. J Cutan Pathol 28:307–313
Said Fernández S, Ramos Guerra MC, Marta Cárdenas BD, Vargas Villarreal J, Villarreal Treviño L (2005) In vitro antiprotozoal activity of the leaves of Artemisia ludoviciana. Fitoter 76:466–468
Schuster FL, Visvesvara GS (1998) Efficacy of novel antimicrobials against clinical isolates of opportunistic amebas. J Eukar Microbiol 45:612–618
Seal DV (2003) Acanthamoeba keratitis update-incidence, molecular epidemiology and new drugs for treatment. Eye 17:893–905
Seal D, Hay J, Kirkness C, Morrell A, Booth A, Tullo A, Ridgway A, Armstrong M (1996) Successful medical therapy of Acanthamoeba keratitis with topical chlorhexidine and propamidine. Eye 10:413–421
Seijo Martinez M, Gonzalez-Mediero G, Santiago P, Rodriguez de Lope A, Diz J, Conde C, Visvesvara GS (2000) Granulomatous amebic encephalitis in a patient with AIDS: isolation of Acanthamoeba sp. group II from brain tissue and successful treatment with sulfadiazine and fluconazole. J Clin Microbiol 38:3892–3895
Singhal T, Bajpai A, Kalra V, Kabra SK, Samantaray JC, Satpathy G, Gupta AK (2001) Successful treatment of Acanthamoeba meningitis with combination oral antimicrobials. Ped Infect Dis J 20:623–627
Sokmen A, Jones BM, Erturk M (1999) The in vitro antibacterial activity of Turkish plants. J Ethnopharm 67:79–86
Tan RX, Zou WX (2001) Endophytes: a rich source of functional metabolites. Nat Prod Rep 18:448–459
Tepe B, Degerli S, Arslan S, Malatyali E, Sarikurkcu C (2011a) Determination of chemical profile, antioxidant, DNA damage drotection and antiamoebic activities of Teucrium polium and Stachys iberica. Fitoter 82:237–246
Tepe B, Malatyali E, Degerli S, Berk S (2011b) In vitro amoebicidal activities of Teucrium polium and T. chamaedrys on Acanthamoeba castellanii trophozoites and cysts. Parasitol Res. doi:10.1007/s00436-011-2698-4
Visvesvara GS (1980) Free-living pathogenic amoebae. In: Lennette EH, Hausler WJ, Truant JP (eds) Manual of clinical microbiology, 3rd edn. Washington, DC: Am Soc Microbiol 704–708
Walochnik J, Duchêne M, Seifert K, Obwaller A, Hottkowitz T, Wiederman G, Eibl H, Aspöck H (2002) Cytotoxic activities of alkylphosphocholines against clinical isolates of Acanthamoeba spp. Antimic Agent Chemother 46:695–701
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This study is financially supported by the Research Council of Cumhuriyet University (CUBAP), Sivas, Turkey with the project number F-346.
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Malatyali, E., Tepe, B., Degerli, S. et al. In vitro amoebicidal activities of Satureja cuneifolia and Melissa officinalis on Acanthamoeba castellanii cysts and trophozoites. Parasitol Res 110, 2175–2180 (2012). https://doi.org/10.1007/s00436-011-2744-2
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DOI: https://doi.org/10.1007/s00436-011-2744-2