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Cyclic AMP decreases the production of NO and CCL2 by macrophages stimulated with Trypanosoma cruzi GPI-mucins

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Abstract

Glycosylphosphatidylinositol-anchored mucin-like glycoproteins (tGPI-mucin) present on the surface of the cellular membrane of Trypanosoma cruzi forms activate toll-like receptors 2 (TLR2) on the surface of immune cells and induce the release of several mediators of inflammation which may be relevant in the context of Chagas disease. Here, we evaluated the ability of tGPI-mucins to activate murine peritoneal macrophages to induce nitric oxide (NO) and monocyte chemoattractant protein-1 (MCP-1/CCL2). We also investigated the ability of compounds which increase or mimic cyclic adenosine monophosphate (AMP) to modulate the production of NO and CCL2. Our data show that elevation of intracellular levels of cyclic AMP prevents the release of NO and CCL2 induced by tGPI-mucins in macrophages. Overall, the release of CCL2 was decreased to a greater extent and at lower concentrations of cyclic AMP-modifying agents than the production of NO. It is suggested that the elevation of cyclic AMP during T. cruzi infection may modify the release of pro-inflammatory mediators and alter significantly the course of T. cruzi infection.

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Acknowledgments

This work was supported by CNPq, FAPEMIG (No. 019/2006) and Rede Mineira de Bioterismo (FAPEMIG). We are grateful to Prof. Ricardo Tostes Gazzinelli for his great assistance with tGPI-mucin used in this work.

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Authors and Affiliations

  1. Laboratório de doença de Chagas, DECBI/NUPEB, Universidade Federal de Ouro Preto, ICEB II, Campus Morro do Cruzeiro–Bauxita, 35400-000, Ouro Preto, MG, Brazil

    Andre Talvani

  2. Departamento de Bioquímica e Imunologia, Instituto Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antonio Carlos, 6627, Pampulha, 31270-901, Belo Horizonte, MG, Brazil

    Sibele Ferreira Coutinho, Luciola da Silva Barcelos & Mauro Martins Teixeira

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  1. Andre Talvani
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  2. Sibele Ferreira Coutinho
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  3. Luciola da Silva Barcelos
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  4. Mauro Martins Teixeira
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Correspondence to Andre Talvani.

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Andre Talvani and Sibele Ferreira Coutinho present equal contribution to this research.

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Talvani, A., Coutinho, S.F., da Silva Barcelos, L. et al. Cyclic AMP decreases the production of NO and CCL2 by macrophages stimulated with Trypanosoma cruzi GPI-mucins. Parasitol Res 104, 1141–1148 (2009). https://doi.org/10.1007/s00436-008-1300-1

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  • DOI: https://doi.org/10.1007/s00436-008-1300-1

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