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High microvessel density in pancreatic ductal adenocarcinoma is associated with high grade

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Abstract

The objectives of this work are to study angiogenesis in pancreatic ductal adenocarcinoma using computerized morphometric and image analysis and to compare the microvascular density in intratumoral and peritumoral areas and normal pancreatic tissue. Microvascular density was analyzed in 60 cases of pancreatic ductal adenocarcinoma and 30 samples of normal pancreatic tissue using an avidin–biotin immunoperoxidase technique with an anti-CD31 antibody. Microvascular density (MVD) was analyzed through digital microimaging and computerized analysis. The blood vessel density in the tumor was significantly higher than in peritumoral areas and in normal pancreatic tissue. Well differentiated pancreatic ductal adenocarcinomas contained higher MVD than poorly differentiated carcinomas. In pancreatic adenocarcinoma, MVD is higher than in peritumoral tissue or normal pancreatic tissue.

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Acknowledgments

This study was carried out with support from the Junta Asociada Provincial of the AECC, Valencia, Spain, and “Gent per Gent” Foundation.

Conflict of interest

We declare that we have no conflict of interest.

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Authors and Affiliations

  1. Department of Internal Medicine, Emergency County Hospital Craiova, University of Medicine and Pharmacy Craiova, Petru Rareş 2, Craiova, 200349, Romania

    Anca Barău

  2. Department of Pathology, University of Valencia, Valencia, Spain

    Amparo Ruiz-Sauri, Gerardo Valencia, Maria del Carmen Gómez-Mateo, Antonio Ferrandez & Antonio Llombart-Bosch

  3. Department of Surgery, Hospital Clinico Universitario de Valencia, Valencia, Spain

    Luis Sabater & Antonio Ferrandez

Authors
  1. Anca Barău
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  2. Amparo Ruiz-Sauri
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  3. Gerardo Valencia
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  4. Maria del Carmen Gómez-Mateo
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  5. Luis Sabater
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  6. Antonio Ferrandez
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  7. Antonio Llombart-Bosch
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Correspondence to Anca Barău.

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Barău, A., Ruiz-Sauri, A., Valencia, G. et al. High microvessel density in pancreatic ductal adenocarcinoma is associated with high grade. Virchows Arch 462, 541–546 (2013). https://doi.org/10.1007/s00428-013-1409-1

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  • DOI: https://doi.org/10.1007/s00428-013-1409-1

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