A reporter mouse line with doxycyclin-inducible expression of β-glucosidase

Abstract

Mouse lines allowing the inducible expression of transgenes became essential tools for studying gene function and for developing accurate animal models for human diseases. A key component of this tool is the availability of “reporter” lines, mice expressing transgenes encoding easily detectable enzymes or other proteins normally not associated with eukaryotic tissues. Such lines may be suitable for a number of applications, including lineage tracing, label-retaining experiments, and the identification and monitoring of regulatory elements important for tissue-specific gene expression. However, only a limited number of reporter lines suitable for inducible expression systems are available. Here, we employed pronuclear DNA microinjection to generate a new reporter mouse line that allows the inducible expression of β-glucosidase, a recently reported stable and easily detectable protein, upon administration of doxycyclin to the drinking water. This novel line was established in the widely used inbreed background C57BL/6, and the transgene is transmitted between generations in a Mendelian fashion. When crossed to a K14-rtTA mouse line, activation of β-glucosidase expression in the epidermal basal layer is easily detected in double-transgenic animals receiving doxycyclin, while no expression is seen in double-transgenic mice without doxycyclin treatment or in animals carrying only one transgene. We anticipate that this new mouse line will become a valuable tool for a number of applications in vivo, including label-retaining experiments and testing the appropriate regulation of rtTA cassettes under different promoters in novel transgenic mouse lines.