Abstract
To obtain single-source short tandem repeat (STR) profiles in trace female/male blood mixture samples, we combined florescence in situ hybridization (FISH), laser microdissection, and low volume PCR (LV-PCR) to isolate male/female cells and improve sensitivity. The results showed that isolation of as few as 10 leukocytes was sufficient to yield full STR profiles in fresh female or male blood samples for 32 independent tests with a low additional alleles rate (3.91 %) and drop-out alleles rate (5.01 %). Moreover, this procedure was tested in two fresh blood mixture series at three ratios (1:5, 1:10, and 1:20), two mock female/male blood mixture casework samples, and one practical casework sample. Male and female STR profiles were successfully detected in all of these samples, showing that this procedure could be used in forensic casework in the future.
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References
Li CX, Wang GQ, Li WS, Huang JP, Ji AQ, Hu L (2011) New cell separation technique for the isolation and analysis of cells from biological mixtures in forensic caseworks. Croat Med J 52(3):293–298
Murray C, McAlister C, Elliott K (2007) Identification and isolation of male cells using fluorescence in situ hybridisation and laser microdissection, for use in the investigation of sexual assault. Forensic Sci Int Genet 1:247–252
Vandewoestyne M, Deforce D (2010) Laser capture microdissection in forensic research: a review. Int J Legal Med 124:513–521
Li CX, Qi B, Ji AQ, Xu XL, Lan H (2009) The combination of single cell micromanipulation with LV-PCR system and its application in forensic science. Forensic Sci Int Genet Suppl Ser 2:516–517
Pettenati MJ, Rao PN, Schnell S, Hayworth-Hodge R, Lantz PE, Geisinger KR (1995) Gender identification of dried human bloodstains using fluorescence in situ hybridization. J Forensic Sci 40:885–887
Gill P, Whitaker J, Flaxman C, Brown N, Buckleton J (2000) An investigation of the rigor of interpretation rules for STRs derived from less than 100 pg of DNA. Forensic Sci 112:17–40
Caragine T, Mikulasovich R, Tamariz J, Bajda E, Sebestyen J, Baum H et al (2009) Validation of testing and interpretation protocols for low template DNA samples using AmpFℓSTR® Identifiler®. Croat Med J 50:250–267
Budowle B, Eisenberg AJ, van Daal A (2009) Validity of low copy number typing and applications to forensic science. Croat Med J 50:207–217
Proff C, Rothschild MA, Schneider PM (2006) Low volume PCR (LV-PCR) for STR typing of forensic casework samples. Int Congr Ser 1288:645–647
Li CX, Han JP, Ren WY, Ji AQ, Xu XL, Hu L (2011) DNA profiling of spermatozoa by laser capture microdissection and low volume-PCR. PLoS One 6(8):e22316
Vandewoestyne M, Van Hoofstat D, Van Nieuwerburgh F, Deforce D (2009) Suspension fluorescence in situ hybridization (S-FISH) combined with automatic detection and laser microdissection for STR profiling of male cells in male/female mixtures. Int J Legal Med 123:441–447
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Authors would like to thank the volunteers who contributed blood samples for analysis.
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Feng, L., Li, Cx., Han, Jp. et al. Isolating cells from female/male blood mixtures using florescence in situ hybridization combined with low volume PCR and its application in forensic science. Int J Legal Med 129, 1211–1215 (2015). https://doi.org/10.1007/s00414-014-1103-0
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DOI: https://doi.org/10.1007/s00414-014-1103-0