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iTRAQ-based quantitative proteomics of stratum corneum of dandruff scalp reveals new insights into its aetiology and similarities with atopic dermatitis

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Abstract

The study aimed at detecting differentially expressed proteins in the stratum corneum of dandruff versus non-dandruff scalps to better understand dandruff aetiology. iTRAQ-based quantitative proteomic analysis revealed a total of 68 differentially expressed biomarkers. A detailed analysis of their known physiological functions provided new insights into the affected metabolic pathways of a dandruff scalp. Dandruff scalp showed (1) profound changes in the expression and maturation of structural and epidermal differentiation related proteins, that are responsible for the integrity of the skin, (2) altered relevant factors that regulate skin hydration, and (3) an imbalanced physiological protease–protease inhibitor ratio. Stratum corneum proteins with antimicrobial activity, mainly those derived from sweat and sebaceous glands were also found modified. Comparing our data with those reported for atopic dermatitis revealed that about 50 % of the differentially expressed proteins in the superficial layers of the stratum corneum from dandruff and atopic dermatitis are identical.

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Abbreviations

iTRAQ:

Isobaric tags for relative and absolute quantitation

SC:

Stratum corneum

DCD 1:

Dermcidin

GGCT:

γ-glutamylcyclotransferase

IDE:

Insulin degrading enzyme

KLK 6:

Kallikrein 6

LVV:

Hemorphin-7

Ly6:

PLAUR domain containing 6

PARK 7:

Parkinson 7

PCA:

Pyrrolidone carboxylic acid

SASpase:

Skin aspartic protease

TGM 3:

Transglutaminase 3

UCA:

Urocanic acid

NMF:

Natural moisturizing factor

PAR-2:

Protease activated receptor 2

AMPs:

Antimicrobial peptides

SERPINs:

Serine protease inhibitors

PIP:

Prolactin-inducible protein

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Acknowledgments

Authors are grateful to Dr. Rainer Schmidt, Dr. Bruno Bernard for their assistance and critical reading of the manuscript and Pr. Reygagne for providing photos of dandruff and non-dandruff scalps.

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  1. L’Oreal Research and Innovation, 1 Avenue Eugène Schueller, 93600, Aulnay-Sous-Bois, France

    Nükhet Cavusoglu, Caroline Delattre, Mark Donovan, Charles El Rawadi, Roland Jourdain & Dominique Bernard

  2. Centre de recherche du CHU de Québec, Plate-forme protéomique, 2705, Boulevard Laurier, QC, Canada

    Sylvie Bourassa & Arnaud Droit

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  1. Nükhet Cavusoglu
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  2. Caroline Delattre
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Correspondence to Nükhet Cavusoglu.

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Cavusoglu, N., Delattre, C., Donovan, M. et al. iTRAQ-based quantitative proteomics of stratum corneum of dandruff scalp reveals new insights into its aetiology and similarities with atopic dermatitis. Arch Dermatol Res 308, 631–642 (2016). https://doi.org/10.1007/s00403-016-1681-4

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