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Analysis of the response of human keratinocytes to Malassezia globosa and restricta strains

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Abstract

Malassezia spp. are saprophyte yeasts involved in skin diseases with different degrees of severity. The aim of our study was to analyze the response of human epidermal keratinocytes to Malassezia globosa and restricta strains evaluating the host defence mechanisms induced by Malassezia spp. colonization. Our results showed a different modulation of the inflammatory and immunomodulatory cytokine pathways obtained with the different strains of Malassezia tested. In addition, this expression is altered by blocking the TLR2 receptor. In comparison with M. furfur, M. globosa and restricta displayed an unexpected and striking cytotoxicity on keratinocytes. The differences observed could be related to the different modalities of interaction between keratinocytes and Malassezia strains, but also to their growth condition. Taken together, these results indicate that M. globosa or M. restricta colonization exert a different control on the cytokine inflammatory response activated in the human keratinocyte in which TLR2 might be involved. M. globosa and M. restricta may play a synergistic role in the exacerbation of skin diseases in which both are found.

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Authors and Affiliations

  1. Microbiology Section, Department of Experimental Medicine, Second University of Naples, Via Costantinopoli, 16, 80138, Naples, Italy

    Giovanna Donnarumma, Brunella Perfetto, Iole Paoletti, Giovanni Oliviero & Maria Antonietta Tufano

  2. L’Oréal Research & Innovation, 1 Avenue Eugène Schueller, 93600, Aulnay sous Bois, France

    Cécile Clavaud, Aurelia Del Bufalo, Audrey Guéniche, Roland Jourdain & Lionel Breton

Authors
  1. Giovanna Donnarumma
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  2. Brunella Perfetto
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  3. Iole Paoletti
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  4. Giovanni Oliviero
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  5. Cécile Clavaud
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  6. Aurelia Del Bufalo
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  7. Audrey Guéniche
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  8. Roland Jourdain
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  9. Maria Antonietta Tufano
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  10. Lionel Breton
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Corresponding author

Correspondence to Maria Antonietta Tufano.

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Donnarumma, G., Perfetto, B., Paoletti, I. et al. Analysis of the response of human keratinocytes to Malassezia globosa and restricta strains. Arch Dermatol Res 306, 763–768 (2014). https://doi.org/10.1007/s00403-014-1479-1

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  • DOI: https://doi.org/10.1007/s00403-014-1479-1

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