Astrocyte TNFR2 is required for CXCL12-mediated regulation of oligodendrocyte progenitor proliferation and differentiation within the adult CNS
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- Patel, J.R., Williams, J.L., Muccigrosso, M.M. et al. Acta Neuropathol (2012) 124: 847. doi:10.1007/s00401-012-1034-0
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Multiple sclerosis (MS) is characterized by episodes of inflammatory demyelination with progressive failure of remyelination. Prior studies using murine models of MS indicate that remyelination within the adult central nervous system (CNS) requires the expression and activity of TNFR2 and CXCR4 by oligodendrocyte progenitor cells (OPCs), promoting their proliferation and differentiation into mature oligodendrocytes. Here, we extend these studies by examining the role of TNFR2 in the expression of the CXCR4 ligand, CXCL12, within the corpus callosum (CC) during cuprizone (CPZ) intoxication and by demonstrating that lentiviral-mediated gene delivery of CXCL12 to the demyelinated CC improves OPC proliferation and myelin expression during remyelination. Activated astrocytes and microglia express both TNFR1 and TNFR2 within the demyelinated CC. However, CPZ intoxicated TNFR2−/− mice exhibit loss of up-regulation of CXCL12 in astrocytes with concomitant decreases in numbers of CXCR4+ NG2+ OPCs within the CC. While CXCR4 antagonism does not affect OPC migration from subventricular zones into the CC, it decreases their proliferation and differentiation within the CC. Stereotactic delivery of lentivirus expressing CXCL12 protein into the CC of acutely demyelinated TNFR2−/− mice increases OPC proliferation and expression of myelin. In contrast, chronically demyelinated wild-type mice, which exhibit significant loss of astrocytes and OPCs, are unable to be rescued via CXCL12 lentivirus alone but instead required engraftment of CXCL12-expressing astrocytes for increased myelin expression. Our results show that TNFR2 activation induces CXCL12 expression in the demyelinated CC via autocrine signaling specifically within astrocytes, which promotes OPC proliferation and differentiation. In addition, gene delivery of critical pro-myelinating proteins might be a feasible approach for the treatment of remyelination failure in MS.
Multiple sclerosis (MS) is an inflammatory, demyelinating disease of the central nervous system (CNS) that presents most often in a relapsing/remitting form, in which a period of demyelination is followed by a period of functional recovery [8, 56]. The recovery stage involves the generation of new myelinating oligodendrocytes, but as the disease worsens, patients exhibit less recovery and accumulate neurological deficits leading to progressive disability. Neurologic functional impairment has been correlated with axonal injury and degeneration, which may occur as a result of remyelination failure or the direct effects of inflammation. While inflammation and remyelination are distinct processes, they are each regulated by master cytokines, such as tumor necrosis factor (TNF-α), that differentially impact infiltrating immune cells and repair of myelin injury. Thus, while successful targeting of TNF-α has led to effective treatments for peripheral autoimmune diseases such as rheumatoid arthritis, psoriasis and inflammatory bowel disease [24, 27, 40], targeting TNF-α in MS patients via administration of a recombinant TNF receptor p55 immunoglobulin fusion protein (lenercept) increased frequency, duration, and severity of MS exacerbations , revealing critical roles for this molecule in recovery.
TNF-α is a pleiotropic cytokine that signals through TNFR1 and TNFR2, inducing apoptotic (TNFR1) and survival pathways (TNFR1 and TNFR2) [4, 40]. MS patients exhibit high levels of TNF-α in cerebrospinal fluid and serum, which are positively correlated with lesion severity [23, 31]. In addition, administration of anti-TNF-α agents or use of mice with targeted deletion of TNFR1 in the murine model of MS, experimental autoimmune encephalitis (EAE), revealed a critical role for this receptor in induction of inflammation . Studies examining the roles of TNF-α receptors in remyelination using a model of demyelination within the adult corpus callosum (CC) induced by the copper chelator cuprizone (CPZ), showed that TNFR2 activity is critical for the proliferation and differentiation of OPCs, resulting in attenuation of remyelination after cessation of CPZ . These data suggest that TNFR2 plays a role in CNS repair of myelin and explain the failure of anti-TNF-α agents to alleviate disease in MS patients.
While the mechanism of TNF-α-mediated remyelination is not known, numerous studies indicate that it induces the expression of the chemokine CXCL12 in various cell types within the CNS [1, 22, 25]. CXCL12 is a pivotal regulator of both leukocyte CNS entry and oligodendrocyte differentiation in the adult CNS [36, 37, 43]. CXCL12 is expressed along vascular ablumina and by activated astrocytes and microglia, leading, respectively, to the perivascular localization of infiltrating leukocytes and to the recruitment and maturation of oligodendrocyte progenitor cells (OPCs), which both express the CXCL12 receptor, CXCR4 [36, 37, 43]. Demyelination in the context of CPZ exposure led to increased expression of CXCL12 within the CC. Additionally, loss of CXCR4 signaling, via pharmacological antagonism or RNA silencing, prevented the maturation of OPCs at this site . In addition, prolonged exposure to CPZ, which leads to chronic demyelination, was associated with lack of CXCL12 up-regulation within the CC . These data led us to hypothesize that remyelination failure in TNFR2-deficient mice was similarly linked to loss of CXCL12 expression. Here we report that TNFR2-deficient mice exhibit lack of up-regulation of CXCL12 within activated astrocytes and lower numbers of CXCR4+ OPCs within the CC during CPZ-induced demyelination. BrDU-labeling studies revealed a role for CXCR4 in the proliferation of NG2+ OPCs within the demyelinated CC. Stereotactic delivery of lentivirus expressing CXCL12 into the CC of acutely demyelinated, TNFR2-deficient mice promoted OPC proliferation and myelin expression. In contrast, mice with chronically demyelinated CC due to prolonged CPZ exposure, which display reduced up-regulation of CXCL12 and decreased numbers of activated astrocytes and OPCs, exhibit lack of remyelination rescue in response to lentiviral delivery of CXCL12. These data identify a critical role for TNFR2+ astrocytes in CXCL12-mediated remyelination and demonstrate that gene delivery approaches may ameliorate failed remyelination.
Materials and methods
TNFR1-/-, TNFR2-/- and C57BL/6 control mice were purchased from Jackson Laboratories (Bar Harbor, ME). Breeder colonies of TNFR1-/-, TNFR2-/-, TNFR1,2-/- were maintained at Washington University. All animal procedures were conducted in compliance with NIH guidelines for the use of laboratory animals and were approved by the Animal Studies Committee of Washington University.
Mouse model of cuprizone-induced demyelination
8- to 10-week old adult male C57BL/6 and TNFR2-/- mice were fed ad libitum 0.2 % CPZ (Harlan Teklan, Madison, WI) mixed in standard rodent chow for 4-12 weeks to induce demyelination of the CC. CPZ-supplemented feed was removed and mice were fed a standard chow diet to induce remyelination .
The following antibodies were used in this study: CXCL12 rabbit polyclonal (eBioscience, San Diego, CA); CXCR4 rat polyclonal (eBioscience); GFAP rat monoclonal (Invitrogen, Carlsbad, CA); IBA1 goat polyclonal (Abcam, Cambridge, MA); MBP rat monoclonal (Abcam); TNFR1 rabbit polyclonal (Abcam); TNFR2 goat polyclonal (R&D systems, Minneapolils, MN); MOG rat monoclonal (R&D systems); BRDU mouse monoclonal (Sigma, St Louis, MO); NG2 rabbit polyclonal (Millipore, Billerica, MA), PDGFRα rat monoclonal (Millipore) and rabbit dsRed for detection of mCherry (Biovision, Milpitas, CA).
Mice were intracardially perfused with 4 % paraformaldehyde (PFA) prior to harvest of the brain, which was post fixed overnight in 4 % PFA, cryoprotected in 20 % sucrose, and embedded in frozen medium for sectioning. For immunodetection of chemokine, chemokine and cytokine receptors, cell markers and mCherry, tissue sections were blocked with appropriate concentrations of goat or donkey serum and triton x and then exposed to primary antibody overnight at 4 °C. Slides were washed in PBS followed by incubation of secondary antibodies for 60 min at room temperature. After washing in PBS, slides were nuclear stained with ToPro-3 (Invitrogen) and coverslip were applied with Prolong Gold (Invitrogen) before being visualized on the Zeiss LSM 510 META Confocal Laser Scanning Microscope (Carl Zeiss International, Jena, Germany). Measurement of positive immunofluorescent signals was accomplished using Volocity (Improvision Waltham, MA, USA) or the public domain NIH Image program ImageJ.
When comparing different treatments (PBS versus AMD3100 or empty vector versus CXCL12 expressing lentivirus) and genotypes (wild-type versus TNFR2-deficient mice) all images were taken at the same exposure settings. To determine the area of double positive labeling, threshold parameters were determined using both the negative controls (isotype control) and images with the lowest fluorescent intensity in both the red and green channels to set the lower threshold setting. Upper threshold settings were not set. The Volocity program was used to determine the area of double labeling and ImageJ was used to determine intensity of signal.
In vivo AMD3100 treatment
AMD3100 treatment was performed as previously published . Briefly, 28-day osmotic pumps with an infusion rate of 0.11 μl/h (Alzet), containing AMD3100 (40 mg/ml) or vehicle (PBS), were implanted subcutaneously into the flanks of mice at the start of CPZ ingestion for proliferation studies or after 7 days after CPZ start for recruitment studies.
In vivo bromodeoxyuridine incorporation
Bromodeoxyuridine (BrDU; Sigma) incorporation experiments were performed as previously described . Briefly, BrDU (50–100 mg/kg) was injected into the peritoneal cavity of experimental mice every 8 h for 4 days. For baseline levels of dividing OPCs and for OPC proliferation experiments, mice were injected in this fashion for 4 days prior to killing whereas OPC migration experiments required killing 3 weeks after BrDU exposure.
All lentiviruses were produced by the Viral Vectors Core Facility of the Hope Center for Neurological Diseases at Washington University School of Medicine. Murine CXCL12 lentivirus vector (FUW-C) and a second vector encoding mCherry protein alone were generated as previously described .
In vivo lentiviral delivery
Delivery of CXCL12 mCherry versus mCherry expressing lentivirus to the CC was accomplished as previously described . Briefly, mice (groups of 3–5) were anesthetized, positioned in a stereotaxic apparatus and a small incision was made to the scalp . The lateral CC was marked using the following coordinates relative to Bregma: anterior–posterior −2, medial–lateral +0.5, and dorsal–ventral −1.2 . Two microliters of lentivirus were injected using a nanoinjector pump (Stoelting, Wood Dale, IL) into the mouse at a rate of 0.2 μl per min. The mice continued on CPZ feed for 5 days after injection to ensure viral infection. Mice were later switched to standard chow for 10 days before evaluation of CNS tissue.
In vivo engraftment
Postnatal astroblasts were cultured from 3-day-old wild-type mouse pups as previously described . After 4 days in culture, the cells were infected with either the CXCL12 mCherry versus mCherry expressing lentivirus for 3 days. The cells were enzymatically detached and 60,000 cells were injected into the CC at the same coordinates as the lentivirus injection experiments. The mice continued on CPZ feed for 5 days after injection to ensure viral infection. Mice were later switched to standard chow for 10 days before evaluation of CNS tissue.
Data were analyzed using Prism software (GraphPad Software). The Student’s t test was used to determine the statistical significance of immunohistochemistry data. P < 0.05 was considered to be significant.
TNFR2 mediates expression of CXCL12 within activated astrocytes during CPZ-induced demyelination
TNFR2-deficient mice exhibit reduced numbers of CXCR4+ OPCs in the CC during CPZ exposure
Delivery of CXCL12-encoding lentivirus rescues remyelination
Given these data, we wondered whether engraftment of CC with astrocytes infected with mCherry and CXCL12-encoding lentivirus would provide the necessary astrocytes to rescue remyelination. Postnatal astroblasts infected with mCherry and CXCL12-encoding or empty vector lentiviruses were stereotactically injected into the CC of wild-type mice after 12 weeks of CPZ exposure. Quantitative confocal IHC evaluation of MBP or MOG expression per area of GFAP+ mCherry+ cells in CC revealed increased expression of MBP (Fig. 6m–o) in the mice engrafted with astrocytes infected with CXCL12-encoding lentivirus versus those receiving astroblasts infected with empty vector virus. These data indicate that engraftment of CXCL12-expressing astrocytes can improve remyelination in the chronically demyelinated mice.
The role of TNF-α in the pathogenesis of CNS autoimmunity is unclear. In studies utilizing the murine MS model, EAE, both TNF-α- and TNFR1-deficient mice are resistant to disease and the use of anti-TNF or sTNF receptor antibodies reduced disease severity [17, 46, 47]. In addition, transgenic mice that over-express TNF-α spontaneously develop chronic, inflammatory demyelinating disease . In human studies, the detection of TNF-α in both the cerebrospinal fluid of MS patients and within MS brain lesions  also suggested it might be targeted to ameliorate disease. However, Phase II clinical trials in which patients with relapsing-remitting MS were administered Lenercept, a sTNF-RI fusion protein that neutralizes TNF-α were halted when disease acutely worsened . The ineffectiveness of anti-TNF-α therapy in humans, contrary to animal studies, suggested divergent roles for TNF-α receptors in inflammation and repair. Both TNFR1 and TNFR2 can exhibit neuroprotective effects. For example TNFR1 has been shown to protect neurons against excitotoxicity by sensitizing the neurons to erythropoietin and vascular endothelial growth factor (VEGF) and TNFR1 signaling can also activate NFκB which induces prosurvival genes [32, 51]. Under certain conditions TNFR1 signaling may activate neuroprotective mechanisms. However, the predominant signaling pathway is not neuroprotective but instead leads to apoptosis . In contrast, TNFR2 signaling is predominantly neuroprotective as shown by the use of TNFR2-selective agonist that rescues neurons from oxidative stress-induced cell death . Consistent with this, TNFR2-deficient mice exhibit delayed repair in models of remyelination  and targeted deletion of tumor necrosis factor receptor associate-factor 4 (TRAF4), a major signal transducer for TNF-α led to ultrastructure perturbation in myelin . These data suggest that therapeutic strategies that antagonize TNFR1 to limit inflammation and augment TNFR2 signaling to enhance remyelination might provide effective treatments that both ameliorate disease and promote recovery in MS patients.
Our results demonstrated that during CPZ-induced demyelination, OPCs express TNFR2 in greater numbers than TNFR1. However, TNFR2-deficiency results in lower numbers of CXCR4+ OPCs within CC after demyelination, suggesting that TNFR2 signaling impacts OPC biology. We and others previously reported that CXCR4 signaling in OPCs is required for their differentiation into myelinating oligodendrocytes in murine models of demyelination [11, 43]. Studies using the CPZ model of demyelination, however, did not address the role of CXCR4 in the recruitment of OPCs from the SVZ. In our current study, we determined that CXCR4 signaling is required for proliferation of OPCs within the CC during demyelination but not for the migration of OPCs to this site. Thus, the localized demyelination of the CC and expression of CXCL12 by astrocytes initiates proliferation of OPCs via CXCR4 signaling directly at the site of the injury. The migration of OPCs from the SVZ is not directed by CXCL12, but instead maybe directed by other chemokines such as CXCL1 and/or CXCL2. Studies have demonstrated that CXCR2, the receptor for CXCL1 and CXCL2, is expressed by OPCs and oligodendrocytes  and has been shown to direct OPC positioning during spinal cord development . TNFα has been shown to induce CXCL1 and CXCL2 in cultured mouse astrocytes and stereotaxic injection of TNF-α to the SJL and BALBc mouse brains induced CXCL2 [18, 42, 55]. Given that we found attenuated CXCL1 message in the TNFR2 deficient mice and decreased numbers of OPCs within the CC, these data suggest that TNFR2 signaling may mediate migration of OPCs via CXCL1 signaling. However, further experiments are necessary to determine the individual contributions of TNFα/TNFR2, CXCL12/CXCR4, and CXCL1/CXCR2 in regulating remyelination.
Other published studies have reported that CXCR4 plays an important role in all aspects of oligodendrocyte biology, including migration. Cultured neonatal OPCs respond to CXCL12 via directed chemotaxis and both CXCR4 and CXCR7, a non-signaling CXCL12 receptor, influence OPC maturation [14, 19]. In in vivo studies, however, Carbajal et al.  reported that CXCR4, but not CXCR7, plays a role in the migration and differentiation of OPCs in a model of chronic demyelination within the spinal cord after primary infection with mouse hepatitis virus (MHV) . Furthermore, in follow-up studies, CXCR4 antagonism was shown to inhibit endogenous OPC maturation and increased the rate of proliferation in the MHV viral model . In the EAE model, Olig2-EGFP+ or CXCR4-EGFP+ oligodendrocyte progenitors implanted into the lateral ventricles of mice at peak of disease migrated into the CC in response to elevated CXCL12 levels and differentiated into mature oligodendrocytes . In addition, migration was inhibited after RNA silencing of CXCR4 . Taken together these reports demonstrate that CXCL12/CXCR4 are important players in mediating OPC biology and an alteration in expression levels of CXCL12 or CXCR4 influences remyelination.
Our results show that TNFR2 deficient mice have reduced CXCL12 expression by astrocytes after CPZ-induced demyelination and that this correlates with reduced OPC numbers. Under normal conditions, astrocytes are a major cellular component of the CNS that provide metabolic and trophic support to maintain homeostasis [49, 53], however, the role of astrogliosis in CNS repair remains controversial. The presence of reactive astrocytes in areas of demyelination has been observed to decrease remyelination within the spinal cord after transplantation of OPCs . In addition, astrocytes isolated from contused spinal cord inhibited the in vitro differentiation of OPCs, whereas astrocytes derived from normal spinal cord did not . However, in several demyelinating models, including cuprizone and EAE, proliferating astrocytes and their secreted factors can promote remyelination in both the spinal cord and CC [16, 38, 39, 43]. Consistent with this, expression of CXCL12 by activated astrocytes within the acutely demyelinated CC influences remyelination by mediating the proliferation and differentiation of OPCs. Reduced astrocyte activation or numbers in the chronically demyelinated CC might therefore negatively influence OPC differentiation and functional myelin formation through loss of a proliferative or trophic microenvironment.
Alterations in astrocyte activity may also have an impact on the success of treatments that attempt to augment or enhance remyelination in MS patients. Lentiviral vectors, for example, are able to preferentially transduce major glial cell types within white matter, such as astrocytes . In our study, CXCL12-encoding lentivirus promoted OPC proliferation and remyelination within the demyelinated CC of TNFR2-deficient mice, which exhibit large numbers of activated astrocytes, but not in the CC of chronically demyelinated mice, which exhibit astrocyte depletion. In addition, as OPC numbers are also reduced during prolonged CPZ treatment, reinstating CXCL12 may exert little effect on their proliferative capacity and ultimate ability to remyelinate. However, further studies are necessary since restoration of depleted OPC and astrocyte populations may require more time to show sufficient remyelination after chronic demyelination. Given the role of CXCL1 on OPC recruitment, lentiviruses expressing multiple chemokines might be required to promote OPC recruitment, proliferation and differentiation within MS lesions. In addition, ex vivo gene therapy, in which cells are genetically modified prior to implantation into the brain, might be employed to replenish depleted astrocytes that augment repair signals . In our study, CXCL12-expressing astrocytes engrafted into the CC of chronically demyelinated showed an increase in myelin expression suggesting that remyelination may be rescued with chemokines and cells that influence chemokine expression in the brain. Such gene therapy or stem cell engraftment approaches may enable the localized manipulation of factors to induce remyelination within various types of MS lesions.
In summary, our data indicate that TNFR2 signaling by activated astrocytes within the demyelinated adult CNS is essential for their expression of CXCL12, which facilitates OPC proliferation and myelin repair. Lentiviral vector-based gene delivery of CXCL12 improved remyelination specifically where loss of astrocytes or OPCs did not prevent lentiviral or CXCL12 targeting. These studies enhance our understanding of cytokine-mediated recovery from demyelination and demonstrate that reinstating chemokine expression may be a feasible approach for the treatment of MS.
This work is supported by National Institutes of Health (NIH)/National Institute of Neurological Disorders and Stroke grant NS059560 and by grants from the National Multiple Sclerosis Society (all to R.S.K).
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