Acta Neuropathologica

, Volume 119, Issue 6, pp 723–735

α-Synucleinopathy in the human olfactory system in Parkinson’s disease: involvement of calcium-binding protein- and substance P-positive cells

Authors

  • Isabel Ubeda-Bañon
    • Laboratorio de Neuroanatomía Humana, Facultad de Medicina/Centro Regional de Investigaciones BiomédicasUniversidad de Castilla-La Mancha
  • Daniel Saiz-Sanchez
    • Laboratorio de Neuroanatomía Humana, Facultad de Medicina/Centro Regional de Investigaciones BiomédicasUniversidad de Castilla-La Mancha
  • Carlos de la Rosa-Prieto
    • Laboratorio de Neuroanatomía Humana, Facultad de Medicina/Centro Regional de Investigaciones BiomédicasUniversidad de Castilla-La Mancha
  • Lucia Argandoña-Palacios
    • Servicio de NeurologíaComplejo Hospitalario Universitario
  • Susana Garcia-Muñozguren
    • Servicio de NeurologíaComplejo Hospitalario Universitario
    • Laboratorio de Neuroanatomía Humana, Facultad de Medicina/Centro Regional de Investigaciones BiomédicasUniversidad de Castilla-La Mancha
Original Paper

DOI: 10.1007/s00401-010-0687-9

Cite this article as:
Ubeda-Bañon, I., Saiz-Sanchez, D., de la Rosa-Prieto, C. et al. Acta Neuropathol (2010) 119: 723. doi:10.1007/s00401-010-0687-9

Abstract

Hyposmia is an early symptom of idiopathic Parkinson’s disease but the pathological bases of such dysfunction are largely unknown. The distribution of α-synuclein, which forms Lewy bodies and Lewy neurites, and the types of neurons (based on their neurotransmitters) affected by α-synucleinopathy were investigated in the olfactory system in Parkinson’s disease. Immunohistochemical distribution of α-synuclein and its co-localization with tyrosine hydroxylase, somatostatin, calbindin, calretinin, parvalbumin and substance P in the olfactory bulb, anterior olfactory nucleus, olfactory tubercle and piriform, periamygdaloid and rostral entorhinal cortices of idiopathic Parkinson’s disease cases (n = 11) and age-matched controls (n = 11) were investigated. Lewy bodies and Lewy neurites were present in the olfactory bulb, particularly in mitral cells and in the inner plexiform layer. α-synuclein was particularly abundant in the different divisions of the anterior olfactory nucleus (bulbar, intrapeduncular, retrobulbar and cortical). In contrast, Lewy bodies and Lewy neurites were less abundant in the olfactory tubercle and olfactory cortices. In the olfactory bulb, anterior olfactory nucleus and olfactory cortices, cells affected by α-synucleinopathy rarely co-localized tyrosine hydroxylase or somatostatin, but they frequently co-localized calbindin, calretinin, parvalbumin and substance P. The present data provide evidence that α-synucleinopathy affects neurons along the olfactory pathway. Dopamine- and somatostatin-positive cells are rarely affected; whereas the cell types most vulnerable to neurodegeneration include glutamate- (mitral cells), calcium-binding protein- and substance P-positive cells. These results provide data on the distribution and cell types involved by α-synucleinopathy in the human olfactory system during Parkinson disease that may be useful for future clinical investigation.

Keywords

α-SynucleinLewy bodyImmunohistochemistryOlfactoryParkinsonHyposmia

Abbreviations

ACC

Nucleus accumbens

AON

Anterior olfactory nucleus

AONb

Bulbar anterior olfactory nucleus

AONcal

Cortical anterior lateral anterior olfactory nucleus

AONcam

Cortical anterior medial anterior olfactory nucleus

AONpal

Cortical posterior lateral anterior olfactory nucleus

AONcpm

Cortical posterior medial anterior olfactory nucleus

AONi

Intrapeduncular anterior olfactory nucleus

AONr

Retrobulbar anterior olfactory nucleus

BL

Basolateral amygdala

BM

Basomedial amygdala

CB

Calbindin

Ce

Central amydala

CR

Calretinin

EC

Entorhinal cortex

EPL

External plexiform layer

GL

Glomerular layer

GCL

Granule cell layer

IPD

Idiopathic Parkinson’s disease

Me

Medial amgydala

MOB

Main olfactory bulb

molf

Medial olfactory radiation

NT

Neurotrace

olfr

Olfactory radiation

ONL

Outer nerve layer

OP

Olfactory peduncle

ot

Olfactory tract

PAC

Periamygdaloid cortex

PD

Parkinson’s disease

Pir

Piriform cortex

PMOL

Posteromedial orbital lobule

PV

Parvalbumin

S

Subiculum

SA

Stratum album

SG

Straight gyrus

SMT

Somatostatin

SP

Substance P

SYN

α-Synuclein

TH

Tyrosine hydroxylase

Supplementary material

401_2010_687_MOESM1_ESM.doc (36 kb)
Supplementary material 1 (DOC 36 kb)

Copyright information

© Springer-Verlag 2010