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Incorporation of Fe@Au nanoparticles into multiresponsive pNIPAM-AAc colloidal gels modulates drug uptake and release

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Abstract

Here, a synthetic method has been optimized for the synthesis of thermoresponsive and pH-responsive poly(N-isopropylacrylamide-co-acrylic acid) nanogels which are subsequently loaded with cytochrome C by using a modified breathing-in mechanism. Physico-chemical properties mapped by using dynamic light scattering (DLS) and differential scanning calorimetry (DSC) confirm the swelling/deswelling kinetics as reversible with a volume phase transition temperature (VPTT) of ~39 °C. Fe@Au nanoparticles were incorporated inside the nanogel networks by using two different methods: coating and in situ growth. The latter bears closer resemblance to the nanogels only, while the former follows the trend of bare Fe@Au nanoparticles. High loading (~96 %) and encapsulation (500 μg/mg of nanogels) of cytochrome C were obtained. Release experiments performed by using a dialysis set-up and monitored by using UV-vis spectroscopy show the highest release at 40 °C and pH 3.2 (high temperature, low pH), with maximum release from the Fe@Au-coated nanogels that also show a reverse swelling-collapse trend. The location of the drug, the incorporation and presence of Fe@Au nanoparticles and the drug incorporation method are found to control both the drug release mechanism and kinetics.

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Acknowledgments

The authors would like to thank NorFab for financial support in connection to the use of NTNU Nanolab and the Faculty of Natural Sciences and Technology, NTNU for financial support.

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Authors and Affiliations

  1. Ugelstad Laboratory, Department of Chemical Engineering, Norwegian University of Science and Technology (NTNU), N-7491, Trondheim, Norway

    Sulalit Bandyopadhyay, Marte Kee Andersen, Muhammad Awais Ashfaq Alvi, Anuvansh Sharma, Birgitte H. McDonagh & Wilhelm Robert Glomm

  2. Department of Chemistry, Norwegian University of Science and Technology (NTNU), N-7491, Trondheim, Norway

    Rajesh Raju

  3. Polymer Particles and Surface Chemistry Research Group, SINTEF Materials and Chemistry, N-7465, Trondheim, Norway

    Wilhelm Robert Glomm

Authors
  1. Sulalit Bandyopadhyay
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  2. Marte Kee Andersen
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  3. Muhammad Awais Ashfaq Alvi
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  4. Anuvansh Sharma
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  5. Rajesh Raju
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  6. Birgitte H. McDonagh
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  7. Wilhelm Robert Glomm
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Correspondence to Sulalit Bandyopadhyay or Wilhelm Robert Glomm.

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Bandyopadhyay, S., Andersen, M.K., Alvi, M.A.A. et al. Incorporation of Fe@Au nanoparticles into multiresponsive pNIPAM-AAc colloidal gels modulates drug uptake and release. Colloid Polym Sci 294, 1929–1942 (2016). https://doi.org/10.1007/s00396-016-3944-1

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  • DOI: https://doi.org/10.1007/s00396-016-3944-1

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