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Size-controlled synthesis of monodisperse core/shell nanogels

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Abstract

Small, monodisperse nanogels (∼50-nm radius) were synthesized by free-radical precipitation polymerization and were characterized using a suite of light scattering and chromatography methods. Nanogels were synthesized with either N-isopropylacrylamide or N-isopropylmethacrylamide as the main monomer, with acrylic acid or 4-acrylamidofluorescein as a comonomer and N,N′-methylenebis(acrylamide) as a cross-linker. By varying the surfactant and initiator concentrations, particle size was controlled while maintaining excellent monodispersity. An amine-containing shell was added to these core particles to facilitate subsequent bioconjugation. Successful conjugation of folic acid to the particles was demonstrated as an example of how such materials might be employed in a targeted drug delivery system.

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Abbreviations

NIPAm:

N-isopropylacrylamide

LCST:

lower critical solution temperature

NIPMAm:

N-isopropylmethacrylamide

AAc:

acrylic acid

EPR:

enhanced permeability and retention

RES:

reticuloendothelial system

APS:

ammonium persulfate

SDS:

sodium dodecyl sulfate

BIS:

N,N′-methylenebis(acrylamide)

APMA:

N-(3-aminopropyl) methacrylamide hydrochloride

EDC:

1-ethyl-3-methyl-(3-dimethylaminopropyl)carbodiimide

DMSO:

dimethyl sulfoxide

AFA:

4-acrylamidofluorescein

MALLS:

multi-angle laser light scattering

AFFF:

asymmetric field flow fractionation

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Acknowledgements

The authors acknowledge financial support from the Department of Health and Human Services (1 R21 EB006499-01) and the Emory-Georgia Tech Nanotechnology Center for Personalized and Predictive Oncology (5-40256-G11).

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Authors and Affiliations

  1. School of Chemistry and Biochemistry & Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA, 30332-0400, USA

    William H. Blackburn & L. Andrew Lyon

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  1. William H. Blackburn
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  2. L. Andrew Lyon
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Correspondence to L. Andrew Lyon.

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Blackburn, W.H., Lyon, L.A. Size-controlled synthesis of monodisperse core/shell nanogels. Colloid Polym Sci 286, 563–569 (2008). https://doi.org/10.1007/s00396-007-1805-7

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  • DOI: https://doi.org/10.1007/s00396-007-1805-7

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