European Journal of Nutrition

, Volume 53, Issue 5, pp 1155–1164

Pomegranate and its derivatives can improve bone health through decreased inflammation and oxidative stress in an animal model of postmenopausal osteoporosis

  • Mélanie Spilmont
  • Laurent Léotoing
  • Marie-Jeanne Davicco
  • Patrice Lebecque
  • Sylvie Mercier
  • Elisabeth Miot-Noirault
  • Paul Pilet
  • Laurent Rios
  • Yohann Wittrant
  • Véronique Coxam
Original Contribution

DOI: 10.1007/s00394-013-0615-6

Cite this article as:
Spilmont, M., Léotoing, L., Davicco, MJ. et al. Eur J Nutr (2014) 53: 1155. doi:10.1007/s00394-013-0615-6

Abstract

Purpose

Recently, nutritional and pharmaceutical benefits of pomegranate (PG) have raised a growing scientific interest. Since PG is endowed with anti-inflammatory and antioxidant activities, we hypothesized that it may have beneficial effects on osteoporosis.

Methods

We used ovariectomized (OVX) mice as a well-described model of postmenopausal osteoporosis to study the influence of PG consumption on bone health. Mice were divided into five groups as following: two control groups sham-operated and ovariectomized (OVX CT) mice fed a standard diet, versus three treated groups OVX mice given a modified diet from the AIN-93G diet, containing 5.7 % of PG lyophilized mashed totum (OVX PGt), or 9.6 % of PG fresh juice (OVX PGj) or 2.9 % of PG lyophilized mashed peel (OVX PGp).

Results

As expected, ovariectomy was associated with a decreased femoral bone mineral density (BMD) and impaired bone micro-architecture parameters. Consumption of PGj, PGp, or PGt induced bone-sparing effects in those OVX mice, both on femoral BMD and bone micro-architecture parameters. In addition, PG (whatever the part) up-regulated osteoblast activity and decreased the expression of osteoclast markers, when compared to what was observed in OVX CT animals. Consistent with the data related to bone parameters, PG consumption elicited a lower expression of pro-inflammatory makers and of enzymes involved in ROS generation, whereas the expression of anti-inflammatory markers and anti-oxidant actors was enhanced.

Conclusion

These results indicate that all PG parts are effective in preventing the development of bone loss induced by ovariectomy in mice. Such an effect could be partially explained by an improved inflammatory and oxidative status.

Keywords

Pomegranate Nutritional prevention Osteoporosis Animal model Inflammation Oxidative stress 

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Mélanie Spilmont
    • 1
    • 2
    • 3
    • 6
  • Laurent Léotoing
    • 1
    • 2
    • 3
  • Marie-Jeanne Davicco
    • 1
    • 2
    • 3
  • Patrice Lebecque
    • 1
    • 2
    • 3
  • Sylvie Mercier
    • 1
    • 2
    • 3
  • Elisabeth Miot-Noirault
    • 4
    • 5
  • Paul Pilet
    • 6
    • 7
  • Laurent Rios
    • 8
  • Yohann Wittrant
    • 1
    • 2
    • 3
    • 9
  • Véronique Coxam
    • 1
    • 2
    • 3
  1. 1.INRA, UMR 1019, UNH, CRNH AuvergneClermont-FerrandFrance
  2. 2.Equipe Alimentation, Squelette et MétabolismesClermont-FerrandFrance
  3. 3.Clermont Université, Université d’AuvergneUnité de Nutrition HumaineClermont-FerrandFrance
  4. 4.Clermont Université, Université d’AuvergneImagerie moléculaire et thérapie vectoriséeClermont-FerrandFrance
  5. 5.Inserm, U 990Clermont-FerrandFrance
  6. 6.Institut National de la Santé et de la Recherche Médicale, UMR S791Laboratoire d’Ingénierie Ostéo-Articulaire et DentaireNantesFrance
  7. 7.Pôle de Recherche et d’Enseignement Supérieur Université Nantes Angers Le Mans, Université de NantesUnité de Formation et de Recherche OdontologieNantesFrance
  8. 8.GREENTECH SA Biopôle Clermont-LimagneSaint-BeauzireFrance
  9. 9.INRA, Equipe ASM, UMR 1019Centre de Recherches INRA de Clermont-Ferrand/TheixClermont-FerrandFrance