Abstract
Purpose
Semaphorin 3A (Sema3A) is a protein secreted during development of the nervous system that plays an important role in neuronal pathophysiology. However, there is no known correlation between Sema3A and intestinal ischemia/reperfusion (I/R) injury. We assessed Sema3A expression and distribution in relation to enteric nervous system (ENS) damage seen after intestinal I/R injury in Sox10-Venus mice.
Methods
Intestinal I/R injury was induced by vascular occlusion for 3 h. Ileal specimens were harvested 0, 3, 12, 24, 48, and 96 h after reperfusion. Stereoscopic microscopy and fluorescence microscopy were used to assess sox10-Venus+ cells and PGP9.5+ cells.
Results
By 3 h after reperfusion, Sema3A expression had increased to a maximum and Sox10-Venus+ cells had faded to a minimum in harvested ileal segments. Both differences were statistically significant. By 96 h after reperfusion, both Sema3A and Sox10-Venus+ cell fluorescence had reverted to original levels. Hematoxylin and eosin staining identified histologic damage mimicking Sema3A expression, while PGP9.5+ cell response was minimal.
Conclusion
We are the first to demonstrate a correlation between Sema3A expression and ENS damage following intestinal I/R in Sox10-Venus mice.
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References
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Acknowledgements
This work was supported by JSPS KAKENHI Grant Number 26462715.
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Takeda, M., Miyahara, K., Okawada, M. et al. Semaphorin 3A expression following intestinal ischemia/reperfusion injury in Sox10-Venus mice. Pediatr Surg Int 33, 383–388 (2017). https://doi.org/10.1007/s00383-016-4039-2
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DOI: https://doi.org/10.1007/s00383-016-4039-2