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Root colonization and effect of biocontrol fungus Paecilomyces lilacinus on composition of ammonia-oxidizing bacteria, ammonia-oxidizing archaea and fungal populations of tomato rhizosphere

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Abstract

This study investigated the effects of root-knot nematode biocontrol agent Paecilomyces lilacinus (P. lilacinus) strain PL1210 on ammonia-oxidizing microorganisms and fungal community composition of tomato rhizosphere. The exchangeable NH4 +-N and NO3 −-N contents were lower in inoculated soils than in the control during 60 days of incubation. Real-time quantitative polymerase chain reaction (qPCR) detected stable colonization of P. lilacinus in the tomato rhizosphere and significant inhibition of ammonia-oxidizing bacteria (AOB) and archaea (AOA), which could be responsible for the decrease of NO3 −-N content in soil. PCR-denaturing gradient gel electrophoresis (DGGE) analysis demonstrated no significant difference in soil fungal community composition associated with the application of P. lilacinus as shown by Shannon–Wiener diversity index (H′) and Margalef index (D). Cluster analysis showed that the composition of rhizosphere fungal community was more significantly influenced by time-related differences than by the inoculation of biocontrol agents.

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Acknowledgments

This work was supported by the 863 - National High Technology Research and Development Program of China (no. 2012AA06A204-2), National Natured Science Foundation (no. 51176197), Xiamen Science & Technology Major Program (no. 3502Z20131018), and Main Project of Chinese Academy Sciences (KZZD-EW-16).

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The authors have no conflict of interest to declare.

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Authors and Affiliations

  1. Institute of Urban Environment, Chinese Academy of Sciences, JiMei Avenue, Xiamen, 361021, China

    Zhen Yu, Youchi Zhang, Wensui Luo & Yin Wang

  2. Graduate University of Chinese Academy of Sciences, Beijing, 100049, China

    Zhen Yu & Youchi Zhang

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  1. Zhen Yu
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  2. Youchi Zhang
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  3. Wensui Luo
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Correspondence to Yin Wang.

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Yu, Z., Zhang, Y., Luo, W. et al. Root colonization and effect of biocontrol fungus Paecilomyces lilacinus on composition of ammonia-oxidizing bacteria, ammonia-oxidizing archaea and fungal populations of tomato rhizosphere. Biol Fertil Soils 51, 343–351 (2015). https://doi.org/10.1007/s00374-014-0983-y

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