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Reduction of intercellular adhesion molecule 1 may play a role in anti-inflammatory effect of hyaluronic acid in a rat model of severe non-bacterial cystitis

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Abstract

Purpose

To evaluate the impact of intercellular adhesion molecule 1 (ICAM-1) in hyaluronic acid (HA) therapy in rats model of severe non-bacterial cystitis.

Methods

Cystitis models in Sprague–Dawley female rats were produced by combination of intraperitoneal cyclophosphamide (CYP) with intravesical protamine/lipopolysaccharide (PS/LPS). HA or heparin (0.5 ml) was introduced intravesically to rats’ bladders followed PS/LPS. Bladder tissue was prepared for histology including mast cell presence and measurement of ICAM-1, tumor necrosis factor (TNF)-α, and interleukin 6 (IL-6).

Results

Cystitis model using intraperitoneal CYP and intravesical SP/LPS showed serious inflammation, higher mast cell count with elevated ICAM-1, TNF-α, and IL-6 levels. After intravesical heparin or HA treatment, incidence of grades 3–4 bladder inflammation and tissue ICAM-1 level were only significantly lower in HA group (P = 0.017, P = 0.021, respectively), but not in heparin group (P = 0.12, P = 0.798, respectively). Remarkably lower level of TNF-α (P = 0.003) and ICAM-1 (P = 0.006) was detected in HA-treated rats compared with heparin-treated rats. Inflammation grade and ICAM-1 level had strong correlation (P < 0.001). IL-6 level after HA or heparin instillation had no difference.

Conclusions

Intravesical administration of HA decreased the severity of bladder inflammation, mast cell presence, and levels of ICAM-1 and TNF-α in a rat model of severe non-bacterial cystitis; its effect was more obvious than that of heparin. Reduction of ICAM-1 may play a role in the anti-inflammatory effect of HA.

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Acknowledgments

The authors express their great thanks to Dr. Yi-fen CUI and Dr. Feng-ying LI for their splendid work in pathological examination and cytokine measurement.

Conflict of interest

The authors declare that they have no conflict of interest or financial disclosure.

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Authors and Affiliations

  1. Department of Urology, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, 197 Ruijin Er Road, Shanghai, 200025, People’s Republic of China

    Yuan Shao, Guo-liang Lu, Zhou-Jun Shen & Hong-chao He

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  1. Yuan Shao
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  2. Guo-liang Lu
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  3. Zhou-Jun Shen
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  4. Hong-chao He
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Correspondence to Yuan Shao.

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Shao, Y., Lu, Gl., Shen, ZJ. et al. Reduction of intercellular adhesion molecule 1 may play a role in anti-inflammatory effect of hyaluronic acid in a rat model of severe non-bacterial cystitis. World J Urol 31, 535–540 (2013). https://doi.org/10.1007/s00345-012-0839-8

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  • DOI: https://doi.org/10.1007/s00345-012-0839-8

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