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Silymarin attenuates the renal ischemia/reperfusion injury-induced morphological changes in the rat kidney

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Abstract

Objectives

Renal ischemia/reperfusion (I/R) injury is associated with increased mortality and morbidity rates due to acute renal failure (ARF). Oxidative stress induced with renal I/R injury directly affects glomerular and tubular epithelium through reactive oxygen species. Several studies have been directed to the treatment of renal I/R injury. The aim of this study was to test the attenuation with silymarin (SM) treatment of renal I/R injury-induced morphological changes in the rat kidney.

Methods

A total of 32 adult male Sprague-Dawley rats were evaluated in four groups. Group I (sham), Group II (renal I/R), Group III (renal I/R injury + SM 50 mg per kg) and Group IV (renal I/R injury + SM 100 mg per kg) were designed to evaluate the dose-dependent effects of SM on the morphological changes of renal I/R injury. Renal I/R injury were induced with left renal pedicle occlusion for 45 min followed with reperfusion for 6 h under anesthesia. After induction of I/R injury, left nephrectomies were performed for histopathological examinations.

Results

After renal I/R injury, significant tubular dilatation, tubular vacuolization, pelvic inflammation, interstitial inflammation, perirenal adipose infiltration, tubular necrosis and glomerular necrosis (cortical necrosis) were observed. However, even with low dose SM in Group III (50 mg per kg SM), histopathological changes due to I/R injury were prevented.

Conclusions

The results of this study have demonstrated that SM significantly prevents renal I/R injury-induced renal tubular changes in the rat. SM in 50 mg/kg was observed to be sufficient to significantly prevent renal tubular necrosis. Further, to our literature knowledge, this is the first specific study to demonstrate the preventive effect of SM on renal I/R injury.

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References

  1. Avlan D, Tamer L, Ayaz L, Polat A, Ozturk C, Ozturhan H, Camdeviren H, Aksoyek S (2006) Effects of trapidil on renal ischemia–reperfusion injury. J Pediatric Surg 41:1686–1693

    Article  Google Scholar 

  2. Thadhani R, Pascual M, Bonvetre JV (1996) Acute renal failure. N Engl J Med 334: 1448–1460

    Article  PubMed  CAS  Google Scholar 

  3. Levy EM, Viscoli CM, Horwitz RI (1996) The effect of acute renal failure on mortality. A cohort analysis. JAMA 275:1489–1494

    Article  PubMed  CAS  Google Scholar 

  4. Rodrigo R, Bosco C (2006) Oxidative stress and protective effects of polyphenols: Comparative studies in human and rodent kidney. A review. Comp Biochem Physiol 142:317–327

    Google Scholar 

  5. Thurman JM (2007) Triggers of inflammation after renal ischemia/reperfusion. Clin Immunol 123:7–13

    Article  PubMed  CAS  Google Scholar 

  6. Sener G, Tugtepe H, Yuksel M, Cetinel S, Gedik N, Yegen BC (2006) Resveratrol improves ischemia/reperfusion-induced oxidative renal injury in rats. Arch Med Res 37:822–829

    Article  PubMed  CAS  Google Scholar 

  7. Gobe G, Johnson DW (2007) Distal tubular epithelial cells of the kidney: Potential support for proximal tubular cell survival after renal injury. Int J Biochem Cell Biol 39: 1551–1561

    Article  PubMed  CAS  Google Scholar 

  8. Havsteen B (1983) Flavonoids, a class of natural products of high pharmacological potency. Biochem Pharmacol 32:1141–1148

    Article  PubMed  CAS  Google Scholar 

  9. Mora A, Paya M, Rios J, Alcaraz M (1990) Structure activity relationships of polymetoxyflavones and other flavonoids as inhibitors of non-enzymatic lipid peroxidation. Biochem Pharmacol 40:793–794

    Article  PubMed  CAS  Google Scholar 

  10. Zhao B (2005) Natural antioxidants for neurodegenerative diseases. Mol Neurobiol 31:283–294

    Article  PubMed  CAS  Google Scholar 

  11. Karimi G, Ramezani M, Tahoonian Z (2005) Cisplatin nephrotoxicity and protection by milk thistle extract in rats. Evid Based Complement Alternat Med 2:383–386

    Article  PubMed  Google Scholar 

  12. Marubayashi S, Dohi K (1996) Therapeutic modulation of free radical-mediated reperfusion injury of the liver and its surgical implications. Surg Today 26:573–580

    Article  PubMed  CAS  Google Scholar 

  13. Granger DN, Korthuis RJ (1995) Physiological mechanisms of postischemic tissue injury. Annu Rev Physiol 57:311–332

    PubMed  CAS  Google Scholar 

  14. Zimmerman BJ, Grisham MB, Granger DN (1990) Role of oxidants in ischemia/reperfusion-induced granulocyte infiltration. Am J Physiol 258:G185–G190

    PubMed  CAS  Google Scholar 

  15. Donnahoo KK, Meng X, Ayala A, Cain MP, Harken AH, Meldrum DR (1999) Early kidney TNF-α expression mediates neutrophil infiltration and injury after renal ischemia–reperfusion. Am J Physiol 277:R922–R929

    PubMed  CAS  Google Scholar 

  16. Weight SC, Bell PR, Nicholson ML (1996) Renal ischemia–reperfusion injury. Br J Surg 83:162–170

    Article  PubMed  CAS  Google Scholar 

  17. Willams P, Lopez H, Britt D, Chan C, Ezrin A, Hottendorf R (1997) Characterization of renal ischemia–reperfusion injury in rats. J Pharmacol Toxicol Methods 37:1–7

    Article  Google Scholar 

  18. Molitoris BA, Mars J (1999) The role of cell adhesion molecules in ischemic acute renal failure. Am J Med 106:583–592

    Article  PubMed  CAS  Google Scholar 

  19. Huang W, Lee SL, Sjöquist M (1995) Natriuretic role of endogenous oxytocin in male rats infused with hypertonic NaCl. Am J Physiol 268:R634–R640

    PubMed  CAS  Google Scholar 

  20. Sehirli AO, Sener G, Satiroglu H, Ayanoglu-Dulger G (2003) Protective effect of N-acetylcysteine on the renal ischemia/reperfusion injury in the rat. J Nephrol 16:75–80

    PubMed  CAS  Google Scholar 

  21. Sener G, Sehirli O, Erkanli G, Cetinel S, Gedik N, Yegen BC (2004) 2-mercaptoethane sulfonate (MESNA) protects against burn-induced renal injury in rats. Burns 30:557–564

    Article  PubMed  Google Scholar 

  22. Ferenci P, Dragosics B, Dittrich H, Frank H, Benda L, Lochs H, Meryn S, Base W, Schneider B (1989) Randomized controlled trial of silymarin treatment in patients with cirrhosis of the liver. J Hepatol 9:105–113

    Article  PubMed  CAS  Google Scholar 

  23. Luper S (1998) A review of plants used in the treatment of liver disease: part 1. Alternat Med Rev 3:410–421

    CAS  Google Scholar 

  24. Chang JW, Kim CS, Kim SB, Park SK, Park JS, Lee SK (2006) Proinflammatory cytokine-induced NF-kappaB activation in human mesangial cells is mediated through intracellular calcium but not ROS: effects of silymarin. Nephron Exp Nephrol 103:e156–e165

    Article  PubMed  CAS  Google Scholar 

  25. Kren V, Walterova D (2005) Silybin and silymarin—new effects and applications. Biomed Papers 149:29–41

    CAS  Google Scholar 

  26. Sonnenbichler J, Zetl I (1986) Biochemical effects of the flavonolignane silibinin on RNA, protein and DNA synthesis in rat livers. Prog Clin Biol Res 213:319–331

    PubMed  CAS  Google Scholar 

  27. Moscarell S, Giusti A, Marra F, Marena C, Lampertico M, Relli P, Gentilini P, Buzzelli G (1993) Therapeutic and antilipoperoxidant effects of silybin phophatidylcholine complex in chronic liver disease: preliminary results. Curr Therapeut Res 53:1

    Article  Google Scholar 

  28. Farghali H, Kameniková L, Hynie S, Kmonicková E (2000) Silymarin effects on intracellular calcuim and cytotoxicity: a study in perfused rat hepatocytes after oxidative stress injury. Pharmacol Res 41:231–237

    Article  PubMed  CAS  Google Scholar 

  29. Jacobs BP, Dennehy C, Ramirez G, Sapp J, Lawrence VA (2002) Milk thistle for the treatment of liver disease: a systemic review and meta-analysis. Am J Med 113:506–515

    Article  PubMed  Google Scholar 

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Conflict of interest statement

There is no conflict of interest.

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Authors and Affiliations

  1. Faculty of Science, Department of Biology, Osmangazi University, Eskisehir, Turkey

    Hakan Senturk, Gokhan Bayramoglu & Ali Kutlu

  2. Faculty of Medicine, Department of Urology, Dumlupinar University, 43100, Kutahya, Turkey

    Sahin Kabay & Mehmet Yucel

  3. Faculty of Medicine, Department of Anatomy, Osmangazi University, Eskisehir, Turkey

    Hilmi Ozden

  4. Faculty of Medicine, Department of General Surgery, Dumlupinar University, Kutahya, Turkey

    Faik Yaylak

  5. Faculty of Medicine, Department of Pathology, Dumlupinar University, Kutahya, Turkey

    Esra Gurlek Olgun

Authors
  1. Hakan Senturk
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  2. Sahin Kabay
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  3. Gokhan Bayramoglu
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  4. Hilmi Ozden
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  5. Faik Yaylak
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  6. Mehmet Yucel
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  7. Esra Gurlek Olgun
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  8. Ali Kutlu
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Correspondence to Sahin Kabay.

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Senturk, H., Kabay, S., Bayramoglu, G. et al. Silymarin attenuates the renal ischemia/reperfusion injury-induced morphological changes in the rat kidney. World J Urol 26, 401–407 (2008). https://doi.org/10.1007/s00345-008-0256-1

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  • DOI: https://doi.org/10.1007/s00345-008-0256-1

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