Abstract
The plant hormone jasmonoyl-isoleucine (JA-Ile) is involved in stress response, development, and secondary metabolite production, although its role in fruit development and ripening remains unknown. The aim of this study is to describe variations of endogenous jasmonate (JAs) contents and JA metabolism-related genes in order to associate these to the evolution of abscisic acid (ABA) content during development and ripening of strawberry (Fragaria × ananassa Duch. cv. Aromas) fruit. A quantitative analysis of phytohormones and gene expression was carried out using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) and quantitative reverse transcription PCR (RT-qPCR) assays, respectively. The expression of genes encoding for JA metabolism enzymes exhibited a significant decrease concomitant to reduction of JAs and an increment of ABA levels from flowering to ripening stages. Exogenous application of methyl jasmonate (MeJA) triggered anthocyanin accumulation along with an increase in JA-Ile, JA, and MeJA levels, and a concomitant decrease in ABA. Together, these results provide insights into JAs homeostasis during strawberry fruit development, suggesting that MeJA-induced anthocyanin accumulation could be mediated by the activation of the JA signaling pathway. Furthermore, we propose an antagonistic relationship from the JA to the ABA pathway during non-climacteric strawberry fruit development and ripening.
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30 October 2017
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Acknowledgements
A.G.-B. acknowledges the support by the CONICYT [Grant ‘Beca Doctorado Nacional 2015 No. 21151411’]. C.R.F. thanks to the CONICYT for the acquisition of the colorimeter and texture analyzer [Grant PIA/ACT-1110]. The authors are grateful for the help of S.V. Smalley in editing the English language throughout the manuscript. In addition, the Danforth Plant Science Center offers their thanks to the National Science Foundation (NSF, USA) for the acquisition of the QTRAP LC-MS/MS [Grant No. DBI-1427621].
Funding
This study was funded by the National Commission for Technological and Scientific Research (CONICYT, Chile) [grant CONICYT, FONDECYT/Regular 1140663 to C.R.F.].
Author Contributions
AG-B conducted experiments, analyzed data, prepared figures and tables, and wrote the manuscript; PMF analyzed data, wrote, and edited the manuscript; and CRF conceived and designed research, obtained research fund, analyzed data, prepared figures and tables, wrote, and edited the manuscript.
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A correction to this article is available online at https://doi.org/10.1007/s00344-017-9755-x.
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Garrido-Bigotes, A., Figueroa, P.M. & Figueroa, C.R. Jasmonate Metabolism and Its Relationship with Abscisic Acid During Strawberry Fruit Development and Ripening. J Plant Growth Regul 37, 101–113 (2018). https://doi.org/10.1007/s00344-017-9710-x
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DOI: https://doi.org/10.1007/s00344-017-9710-x