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Retreived bacteria from Noctiluca miliaris (green) bloom of the northeastern Arabian Sea

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Abstract

In recent years, seasonal blooms of the dinoflagellate Noctiluca miliaris have appeared in the open-waters of the northern Arabian Sea (NAS). This study provides the first characterization of bacteria from a seasonal bloom of green Noctiluca of NAS (20°N−17°N and 64°E–70°E), during the spring-inter-monsoon cruise of Sagar Sampada 253, in March 2007. Bacterial growth as assessed by most-probable number (MPN) and plate counts, revealed ‘variable-physiotypes’ over a wide range of salinities (0%–25% w/v NaCl), pH levels (5–8.5), and organic nutrient strengths, in comparison to non-bloom waters. MPN indices of bacteria in surface waters of bloom stations *DWK and *PRB, corresponded to (3.08–4.41)×103 cells/mL at 3.5% NaCl (w/v), and (2.82–9.49)×102 cells/mL at 25% (w/v) NaCl in tryptone-yeast extract broth (TYE). Plate counts were (1.12–4)×106 CFU/mL at 0% (w/v) NaCl, (1.28–3.9)×106 CFU/mL at 3.5% (w/v) NaCl, and (0.4–7)×104 CFU/mL at 25% NaCl (w/v) on TYE. One-tenth-strength Zobell’s gave (0.6–3.74)×105 CFU/mL at pH 5 to (3.58–7.5)×105 CFU/mL at pH 8.5. These bacteria were identified to the genera Bacillus, Cellulomonas, Staphylococcus, Planococcus, Dietzia, Virgibacillus, Micrococcus, Sporosarcinae, Leucobacter, and Halomonas. The identity of three strains (GUFBSS253N2, GUFBSS253N30, and GUFBSS253N84) was confirmed through 16S rDNA sequence homology as Bacillus cohnii, Bacillus flexus, and Bacillus cereus. The ∼2–3-fold higher plate counts of culturable bacteria from the open-waters of the NAS indicate that these bacteria could critically determine the biogeochemical dynamics of the bloom and its milieu. The role of these bacteria in sustaining/terminating the bloom is under evaluation.

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Authors and Affiliations

  1. Department of Microbiology, Goa University, Taleigao Plateau, Taleigao, Goa, 403206, India

    Subhajit Basu & Irene Furtado

  2. National Institute of Oceanography (CSIR-India), Dona-Paula, Goa, 403004, India

    S. G. Prabhu Matondkar

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  1. Subhajit Basu
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Correspondence to Irene Furtado.

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Basu, S., Matondkar, S.G.P. & Furtado, I. Retreived bacteria from Noctiluca miliaris (green) bloom of the northeastern Arabian Sea. Chin. J. Ocean. Limnol. 31, 10–20 (2013). https://doi.org/10.1007/s00343-013-2017-2

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