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A harpacticoid copepod Microsetella spp. from sub-Arctic coastal waters and its sensitivity towards the polyaromatic hydrocarbon pyrene

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Abstract

Species of the harpacticoid copepod genus Microsetella are commonly reported to occur in Arctic and sub-Arctic coastal waters, but nothing has yet been reported on their sensitivity towards toxic substances. Effects of the polyaromatic hydrocarbon pyrene on Microsetella spp. from Western Greenland were investigated by looking at survival of females, feeding status, and nucleic acid content after a 96-h exposure. Less than 10% survived at 100 nM exposure concentration, and a 50% reduced survival was also evident at 0.1 and 10 nM. The RNA:DNA ratio was significantly higher, 2.1 ng/individual, at the lowest concentration of 0.01 nM compared to 0.9 ng/individual in the control, which indicates increased metabolic activities. A reduced DNA content in higher exposure concentrations of 1–100 nM suggests inhibition of egg development. Gut content was significantly declining with increasing exposure concentrations with only 2% of exposed females displaying full guts at 100 nM pyrene. Together with reduced feeding activity, effects at the population level can be anticipated, but would have to be confirmed in future experiments. The data suggests a higher sensitivity of Microsetella spp. compared to other Arctic copepods, which implies more severe effects from oil on the pelagic food web in the areas and periods where Microsetella spp. dominates Arctic plankton food webs.

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References

  • Barata C, Calbet A, Saiz E, Ortiz L, Bayona JS (2005) Predicting single and mixture toxicity of petrogenic polycyclic aromatic hydrocarbons to the copepod Oithona davisae. Environ Toxicol Chem 24:2992–2999

    Article  PubMed  CAS  Google Scholar 

  • Bignert A, Cossa D, Emmerson R, Fryer R et al (2004) OSPAR/ICES workshop on the evaluation and update of background reference concentrations (B/RCs) and ecotoxicological assessment criteria (EACs) and how these assessment tools should be used in assessing contaminants in water, sediment, and biota. Workshop, The Hague, 9–13 February 2004. Final Report. OSPAR Commission

  • Buchmann MF (1999) NOAA screening quick reference tables, NOAA HAZMAT report 99-1, Seattle, WA, Coastal Protection and Restoration Division, National Oceanic and Atmospheric administration

  • Chapman PM, Riddle MJ (2005) Toxic effects of contaminants in polar marine environments. Environ Sci Technol 39(9):200A–207A

    Article  PubMed  CAS  Google Scholar 

  • Dachs J, Bayona JM, Raoux C, Albaiges J (1997) Spatial, vertical distribution and budget of polycyclic aromatic hydrocarbons in the western Mediterranean seawater. Environ Sci Technol 31:682–688

    Article  CAS  Google Scholar 

  • Dahl U, Gorokhova E, Breitholtz M (2006) Application of growth-related sublethal endpoints in ecotoxicological assessments using a harpacticoid copepod. Aquat Toxicol 77:433–438

    Article  PubMed  CAS  Google Scholar 

  • Dahlhoff EP (2004) Biochemical indicators of stress and metabolism: applications for marine ecological studies. Ann Rev Physiol 66:183–207

    Article  CAS  Google Scholar 

  • Dugas JC, Koslow JA (1984) Microsetella norvegica: a rare report of a potentially abundant copepod on the Scotian Shelf. Mar Biol 84:131–134

    Article  Google Scholar 

  • Evans F, Diaz W (1978) Microsetella norvegica (Boeck): a direct relationship between seasonal sea temperature and adult size in a planktonic copepod. Crustaceana 34(3):313–315

    Article  Google Scholar 

  • Fish CJ (1955) Observations on the biology of Microsetella norvegica. Pap Mar Biol Oceanogr Deep Sea Res 3(Suppl.):242–249

    Google Scholar 

  • Gorokhova E (2003) Relationships between nucleic acid levels and egg production rates in Acartia bifilosa: implications for growth assessment of copepods in situ. Mar Ecol Prog Ser 262:163–172

    Article  CAS  Google Scholar 

  • Gorokhova E, Kyle M (2002) Analysis of nucleic acids in Daphnia: development of methods and ontogenetic variations in RNA–DNA content. J Plankton Res 5:511–522

    Article  Google Scholar 

  • Green EP, Dagg MJ (1997) Mesozooplankton associations with medium to large marine snow aggregates in the northern Gulf of Mexico. J Plankton Res 19(4):435–447

    Article  Google Scholar 

  • Greve W, Reiners F, Nast J, Hoffmann S (2004) Helgoland Roads meso- and macrozooplankton time-series 1974 to 2004: lessons from 30 years of single spot, high frequency sampling at the only off-shore island of the North Sea. Helgol Mar Res 58:274–288

    Article  Google Scholar 

  • Halliday NC, Coombs SH, Smith C (2001) A comparison of LHPR and OPC data from vertical distributions sampling of zooplankton in a Norwegian fjord. Sarsia 86:87–99

    Google Scholar 

  • Jensen MH, Nielsen TG, Dahllöf I (2008) Effects of pyrene on grazing and reproduction of Calanus finmarchicus and Calanus glacialis from Disco Bay, West Greenland. Aquat Toxicol 87:99–107

    Article  PubMed  CAS  Google Scholar 

  • Khan I, Islam MR (2005) Assessing the environmental fate and behaviour of oil discharges in the marine ecosystem using the fugacity model. In: Armsworthy SL, Cranford PJ, Lee K (eds) Offshore oil and gas environmental effects monitoring: approaches and technologies. Battelle Press, Columbus

    Google Scholar 

  • Koski M, Kiørboe T, Takahashi K (2005) Benthic life in the pelagic: aggregate encounter and degradation rates by pelagic harpacticoid copepods. Limnol Oceanogr 50(4):1254–1263

    Google Scholar 

  • Koski M, Møller EF, Maar M, Visser AW (2007) The fate of discarded appendicularian houses: degradation by the copepod, Microsetella norvegica, and other agents. J Plankton Res 29(7):641–654

    Article  CAS  Google Scholar 

  • Latimer JS, Zheng J (2003) The sources, transport and fate of PAHs in the marine environment. In: Douben PET (ed) PAHs: an ecotoxicological perspective. Wiley, London

    Google Scholar 

  • Law RJ, Dawes VJ, Woodhead RJ, Matthiessen P (1997) Polycyclic aromatic hydrocarbons (PAH) in seawater around England and Wales. Mar Pollut Bull 34(5):306–322

    Article  CAS  Google Scholar 

  • Lee RF, Hagen W, Kattner G (2006) Lipid storage in marine zooplankton. Mar Ecol Prog Ser 307:273–306

    Article  CAS  Google Scholar 

  • Lorenzen CJ (1967) Determination of chlorophyll and pheopigments: spectrophotometric equations. Limnol Oceanogr 12:343–346

    Article  CAS  Google Scholar 

  • Lotufo GR (1998) Lethal and sublethal toxicity of sediment-associated fluoranthene to benthic copepods: application of the critical-body-residue approach. Aquat Toxicol 1/2:17–30

    Google Scholar 

  • Maar M, Visser AW, Nielsen TG, Stips A, Saito H (2006) Turbulence and feeding behaviour affect the vertical distributions of Oithona similis and Microsetella norvegica. Mar Ecol Prog Ser 313:157–172

    Article  Google Scholar 

  • Madsen SD, Nielsen TG, Hansen BW (2001) Annual population development and production by Calanus finmarchicus, C. glacialis and C. hyperboreus in Disko Bay, western Greenland. Mar Biol 139(1):75–93

    Article  Google Scholar 

  • Meyer B, Irigoien X, Graeve M, Head RN, Harris RP (2002) Feeding rates and selectivity among nauplii, copepodites and adult females of Calanus finmarchicus and Calanus helgolandicus. Helgol Mar Res 56:169–176

    Article  Google Scholar 

  • Møller EF, Nielsen TG, Richardson K (2005) The zooplankton community in the Greenlandic Sea: composition and role of carbon turnover. Deep-Sea Res I 53:76–93

    Google Scholar 

  • Nielsen TG, Andersen MC (2002) Plankton community structure and production along a freshwater-influenced Norwegian fjord system. Mar Biol 141:707–724

    Article  Google Scholar 

  • Nielsen TG, Hansen B (1995) Plankton community structure and carbon cycling on the western coast of Greenland during and after the sedimentation of a diatom bloom. Mar Ecol Prog Ser 125:239–257

    Article  CAS  Google Scholar 

  • Oresland V, Bray RA (2005) Parasites and headless chaetognaths in the Indian Ocean. Mar Biol 147(3):725–734

    Article  Google Scholar 

  • Pedersen SA, Ribergaard MH, Simonsen CS (2005) Micro- and mesozooplankton in Southwest Greenland waters in relation to environmental factors. J Mar Syst 56:85–112

    Article  Google Scholar 

  • Petersen DG, Dahllöf I (2007) Combined effects of pyrene and UV-light on algae and bacteria in an arctic sediment. Ecotoxicology 16(4):371–377

    Article  PubMed  CAS  Google Scholar 

  • Petersen DG, Dahllöf I, Nielsen LP (2004) Effects of zinc pyrithione and copper pyrithione on microbial community function and structure in sediments. Environ Toxicol Chem 23(4):921–928

    Article  CAS  Google Scholar 

  • Rudnick SM, Chen RF (1998) Laser-induced fluorescence of pyrene and other polycyclic aromatic hydrocarbons (PAH) in seawater. Talanta 47:907–919

    Article  CAS  PubMed  Google Scholar 

  • Saiz E, Calbet A, Fara A, Berdalet E (1998) RNA content of copepods as a tool for determining adult growth rates in the field. Limnol Oceanogr 43:465–470

    CAS  Google Scholar 

  • Snyder MJ (2000) Cytochrome P450 enzymes in aquatic invertebrates: recent advances and future directions. Aquat Toxicol 48(4):529–547

    Article  PubMed  CAS  Google Scholar 

  • Takahashi K, Kawaguchi K (1998) Diet and feeding rhythm of the sand-burrowing mysids Archaeomysis kokuboi and A. japonica in Otsuchi Bay, northeastern Japan. Mar Ecol Prog Ser 162:191–199

    Article  Google Scholar 

  • Turner JT (2004) The importance of small planktonic copepods and their roles in pelagic marine food webs. Zool Stud 43(2):255–266

    Google Scholar 

  • Uye S, Aoto I, Onbé T (2002) Seasonal population dynamics and production of Microsetella norvegica, a widely distributed but little-studied marine planktonic harpacticoid copepod. J Plankton Res 24(2):143–153

    Article  Google Scholar 

  • Vinas MD, Ramirez FC (1996) Gut analysis of first-feeding anchovy larvae from the Patagonian spawning areas in relation to food availability. Arch Fish Mar Res 43(3):231–256

    Google Scholar 

  • Walkusz W, Kwasniewski S, Dmoch K, Hop H, Zmijewska MI, Bielecka L, Falk-Petersen S, Scinski J (2004) Characteristics of the Arctic and Antarctic mesozooplankton in the neritic zone during summer. Pol Polar Res 25(3/4):275–291

    Google Scholar 

  • Witt G (2002) Occurrence and transport of polycyclic aromatic hydrocarbons in the water bodies of the Baltic Sea. Mar Chem 79:49–66

    Article  CAS  Google Scholar 

  • Young JW, Davis TLO (1992) Feeding ecology and interannual variations in diet of larval jack mackerel, Trachurus declivis (Pisces, Carangidae), from coastal waters of eastern Tasmania. Mar Biol 113(1):11–20

    Article  Google Scholar 

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Acknowledgments

The Department of Arctic Technology at the Danish Technical University Sisimiut, Greenland, is gratefully acknowledged for providing laboratory facilities, and Morten Holtegaard Nielsen, also at ARKTEK/DTU, is acknowledged for assisting with CTD data.

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  1. Department of Marine Ecology, National Environmental Research Institute, University of Aarhus, Frederiksborgvej 399, 4000, Roskilde, Denmark

    Morten Hjorth & Ingela Dahllöf

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  1. Morten Hjorth
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  2. Ingela Dahllöf
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Correspondence to Morten Hjorth.

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Hjorth, M., Dahllöf, I. A harpacticoid copepod Microsetella spp. from sub-Arctic coastal waters and its sensitivity towards the polyaromatic hydrocarbon pyrene. Polar Biol 31, 1437–1443 (2008). https://doi.org/10.1007/s00300-008-0483-3

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  • DOI: https://doi.org/10.1007/s00300-008-0483-3

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