Abstract
We investigated the renal morphology, histology and ultrastructure of Harpagifer bispinis, as a first step toward understanding the morpho-functional basis of its adaptation to potentially freezing brackish seawater. Fish were separated into two groups of ten individuals each, and acclimated to 2‰ and 38‰ salinity. A study of complete serial sections of the kidney revealed that the nephrons were aglomerular. At the highly convoluted proximal segment two different regions were evident, a feature that has not been previously reported for other aglomerular species. In electron photomicrographs we distinguished light and dark cells in the proximal tubule epithelium, with highly infolded basolateral membranes and closely associated mitochondria. The dark cells also had a large number of mitochondria in the apical region. The intercellular spaces at the epithelium of the proximal tubule were larger in fish acclimated at 2‰ salinity, a modification that might facilitate urine secretion, thus contributing to the survival of an aglomerular fish in a hyposmotic medium.
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References
Baustein MD, Wang SQ, Beyenbach KW (1997) Adaptive responses of aglomerular toadfish to dilute seawater. J Comp Physiol B 167:61–69
Bulger RE (1965) The fine structure of the aglomerular nephron of the toadfish, Opsanus tau. Am J Anat 117:171–192
Bulger RE, Trump BF (1968) Renal morphology of the English sole (Parophrys vetulus). Am J Anat 123:195–226
Clarke A, Johnston IA (1996) Evolution and adaptative radiation of Antarctic fishes. Trends Evol Ecol 11:212–218
DeVries AL (1982) Biological antifreeze agents in coldwater fishes. Comp Biochem Physiol A73:627–266
Dobbs GH, DeVries AL (1975a) Renal function in Antarctic teleost fish: serum and urine composition. Mar Biol 29:59–70
Dobbs GH, DeVries AL (1975b) The aglomerular nephron of Antarctic teleost: a light and electron microscopic study. Tissue Cell 7:159-170
Dobbs GH, Lin Y, DeVries AL (1974) Aglomerularism in Antarctic fish. Science 185:793–794
Eastman JT (1993) Antarctic fish biology. Evolution in a unique environment. Academic, San Diego
Eastman JT, DeVries A (1986a) Renal glomerular evolution in Antartic notothenioid fishes. J Fish Biol 29:649–662
Eastman JT, DeVries A (1986b) Antarctic fish. Sci Am 254:106–114
Elger M, Hentschel H, Dawson M, Renfro JL (2000) Microscopic functional anatomy: urinary tract. In: Ostrander G (ed) The laboratory fish. Academic, New York, pp 385–413
Ernst SA, Mills JW (1977) Basolateral plasma membrane localization of ouabain-sensitive sodium transport sites in the secretory epithelium of the avian salt gland. J Cell Biol 75(1):74–94
Fernández D (2000) Histoquímica, distribución y crecimiento de las fibras musculares en nototénidos subantárticos. Análisis inicial de dos factores relacionados: flotabilidad y temperatura. Universidad de Buenos Aires. Graduate thesis
Fischer W, Hureau JC (1985) FAO species identification sheets for fishery purposes. Southern Ocean (Fishing areas 48,58 and 88) (CCAMLR Convention Area). Prepared and published with the support of the Commission for the Conservation of Antarctic Marine Living Resources. FAO, Rome, vol 2, pp 233–470
Gonzalez-Cabrera PJ, Dowd F, Pedibhotla VK, Rosario R, Stanley-Samuelson D, Petzel D (1995) Enhanced hypo-osmoregulation induced by warm-acclimation in Antarctic fish is mediated by increased gill and kidney Na+/K+-ATPase activities. J Exp Biol 198:2279–2291
Hickman CP, Trump BF (1969) The kidney. In: Hoar WS, Randall DJ (eds) Fish physiology, vol 1. Academic, New York, pp 91–239
Lahlou B, Henderson W, Sawyer WH (1969) Renal adaptations by Opsanus tau, a euryhaline aglomerular teleost, to dilute media. Am J Physiol 216:1266–1272
Massini MA, Sturla M, Prato P, Uva B (2001) Ion transport systems in the kidney and urinary bladder of two Antarctic teleosts, Chionodraco hamatus and Trematomus bernacchii. Polar Biol 24:440–446
Ogawa M (1961) Comparative study of the external shape of the teleostean kidney with relation to phylogeny. Sci Rep Tokyo Kyoiku Daigaku Sect B10:61–69
Olsen S, Ericsson JLE (1968) Ultrastructure of the tubule of the aglomerular teleost Nerophis ophidion. Z Zellforsch 87:17–30
Pérez A, Luquet C, Calvo J (2001) Morfología renal de los nototenoideos subantárticos Harpagifer bispinis y Patagonotothen tessellata. Aglomerulismo en un pez eurihalino. Congreso Brasileño de Ictiología. San Leopoldo, Rio Grande Do Sul, Brazil
Perry SF, Fryer JN (1997) Proton pumps in the fish gill and kidney. Fish Physiol Biochem 17:363–369
Romão S, Freire CA, Fanta E (2001) Ionic regulation and Na+, K+-ATPase activity in gills and kidney of the Antarctic aglomerular cod icefish exposed to dilute sea water. J Fish Biol 59:463–468
Schmidt-Nielsen K (1976) Regulación del agua y regulación osmótica. In: Fisiología animal. Adaptación y medio ambiente. Ediciones Omega, Barcelona, pp 263–312
Trump BF, Bulger RE (1971) Experimental modification of lateral and basilar plasma membranes and extracellular compartments in the flounder nephron. Fed Proc Fed Am Soc Exp Biol 30:22–41
Wendelaar Bonga SE (1973) Morphometrical analysis with the light and electron microscope of the kidney of the anadromous three-spined stickleback Gasterosteus aculeatus, form trachurus, from fresh water and from sea water. Z Zellforsch Mikrosk Anat 137:563–588
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We wish to thank Martin Ansaldo, Elba Morriconi, Daniel Aureliano, Fabian Vanella, Griselda Genovese and Julia Halperin for their kind help.
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Pérez, A.F., Calvo, J., Tresguerres, M. et al. Aglomerularism in Harpagifer bispinis: a subantarctic notothenioid fish living at reduced salinity. Polar Biol 26, 800–805 (2003). https://doi.org/10.1007/s00300-003-0551-7
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DOI: https://doi.org/10.1007/s00300-003-0551-7