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Analysis of Genetic Diversity and Development of SCAR Markers in a Mycogone perniciosa Population

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Abstract

The fungus Mycogone perniciosa is a major pathogen of the common button mushroom Agaricus bisporus. Analysis of genetic diversity in M. Perniciosa may assist in developing methods for prophylaxis and treatment of M. Perniciosa infections. For this, it is necessary to classify M. Perniciosa into relevant class groups quickly and efficiently. Random amplified polymorphic DNA (RAPD), inter-simple sequence repeats (ISSR), and sequence-related amplified polymorphism (SRAP) markers were used to obtain genetic fingerprints and assess the genetic variation among 49 strains of M. perniciosa collected from different areas of Fujian Province in China. Analysis of DNA sequence polymorphism revealed two major distinct groups (Group I and Group II). Specific DNA fragments that were identified through RAPD, ISSR, and SRAP markers were sequenced and used for the designing of stable sequence-characterized amplified region (SCAR) markers. The resulting SCAR markers were then validated against the classified groups of M. perniciosa.

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Acknowledgments

This research was supported by the Key Technologies R&D Program of China (No. 2013BAD16B03). The authors are grateful to the Fujian Edible Fungi Engineering Technology Research Center and National Edible Fungi Breeding Center (Fujian Division) for providing experimental facilities. We also thank Dr. Arend F. van Peer for language editing of the manuscript.

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Authors and Affiliations

  1. Mycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China

    Wei Wang, Xiao Li, Bingzhi Chen, Shuang Wang, Chenghuan Li & Zhiqiang Wen

  2. College of Food Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China

    Bingzhi Chen

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  1. Wei Wang
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  2. Xiao Li
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  3. Bingzhi Chen
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  4. Shuang Wang
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Correspondence to Zhiqiang Wen.

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Wang, W., Li, X., Chen, B. et al. Analysis of Genetic Diversity and Development of SCAR Markers in a Mycogone perniciosa Population. Curr Microbiol 73, 9–14 (2016). https://doi.org/10.1007/s00284-016-1020-1

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  • DOI: https://doi.org/10.1007/s00284-016-1020-1

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