Skip to main content
SpringerLink
Log in

Co-spinning of Silver Nanoparticles with Nisin Increases the Antimicrobial Spectrum of PDLLA: PEO Nanofibers

  • Published:
Current Microbiology Aims and scope Submit manuscript

Abstract

Silver nanoparticles (AgNPs), synthesized using N,N-dimethylformamide (DMF), were electrospun with nisin in a 50:50 blend of 24 % (w/v) poly(d,l-lactide) (PDLLA) and poly(ethylene oxide) (PEO). Addition of AgNPs decreased the average diameter of the nanofibers [silver nanofibers (SF)] from 588 ± 191 to 281 ± 64 nm, or to 288 ± 63 nm when nisin was co-spun with AgNPs. Nanofibers containing AgNO3 (SF) had a beads-on-string structure, whereas nanofibers with AgNPs and nisin [silver plus nisin nanofibers (SNF)], nanofibers with only nisin [nisin nanofibers (NF)], and nanofibers without AgNPs and nisin [control nanofibers] had a uniform structure. The irregular topography was confirmed by atomic force microscopy. No interactions occurred between silver, nisin, PDLLA, and PEO, as confirmed with Fourier transform infrared spectroscopy. Most of the AgNPs (18 ± 2.8 ppm) and nisin (78.1 ± 1.2 µg/ml) were released within the first 2 h. SF and SNF inhibited the growth of gram-positive and gram-negative bacteria, whereas NF failed to inhibit gram-negative bacteria. A wound dressing with broad-spectrum antimicrobial activity may be developed by the incorporation of nanofibers containing a combination of AgNPs and nisin.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Ahire JJ, Dicks LMT (2014) Nisin incorporated with 2,3-dihydroxybenzoic acid in nanofibers inhibits biofilm formation by a methicillin-resistant strain of Staphylococcus aureus. Probiotics Antimicrob Proteins 7:52–59

    Article  Google Scholar 

  2. Ahire JJ, Neppalli R, Heunis TD, van Reenen AJ, Dicks LMT (2014) 2, 3-dihydroxybenzoic acid electrospun into poly(d,l-lactide) (PDLLA)/poly (ethylene oxide) (PEO) nanofibers inhibited the growth of gram-positive and gram-negative bacteria. Curr Microbiol 69(5):587–593

    Article  CAS  PubMed  Google Scholar 

  3. Ahire JJ, Dicks LMT (2014) 2,3-Dihydroxybenzoic acid-containing nanofiber wound dressings inhibits biofilm formation by Pseudomonas aeruginosa. Antimicrob Agents Chemother 58(4):2098–2104

    Article  PubMed Central  PubMed  Google Scholar 

  4. Borase HP, Salunke BK, Salunkhe RB, Patil CD, Hallsworth JE, Kim BS, Patil SV (2014) Plant extract: a promising biomatrix for ecofriendly, controlled synthesis of silver nanoparticles. Appl Biochem Biotechnol 173:1–29

    Article  CAS  PubMed  Google Scholar 

  5. Carmona-Ribeiro AM, de Melo Carrasco LD (2014) Novel formulations for antimicrobial peptides. Int J Mol Sci 15(10):18040–18083

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  6. Chernousova S, Epple M (2013) Silver as antibacterial agent: ion, nanoparticle, and metal. Angew Chem Int Ed 52:1636–1653

    Article  CAS  Google Scholar 

  7. Chopra I (2007) The increasing use of silver-based products as antimicrobial agents: a useful development or a cause for concern? J Antimicrob Chemother 59(4):587–590

    Article  CAS  PubMed  Google Scholar 

  8. Dolina J, Jiříček T, Lederer T (2013) Membrane modification with nanofiber structures containing silver. Ind Eng Chem Res 52(39):13971–13978

    Article  CAS  Google Scholar 

  9. Fouda MM, El-Aassar MR, Al-Deyab SS (2013) Antimicrobial activity of carboxymethyl chitosan/polyethylene oxide nanofibers embedded silver nanoparticles. Carbohydr Polym 92(2):1012–1017

    Article  CAS  PubMed  Google Scholar 

  10. Heunis TDJ, Smith C, Dicks LMT (2013) Evaluation of a nisin-eluting nanofiber scaffold to treat Staphylococcus aureus-induced skin infections in mice. Antimicrob Agents Chemother 57:3928–3935

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  11. Heunis TDJ, Dicks LMT (2010) Nanofibers offer alternative ways to the treatment of skin infections. J Biomed Biotechnol 61:1–10

    Article  Google Scholar 

  12. Kopermsub P, Mayen V, Warin C (2012) Nanoencapsulation of nisin and ethylenediamine tetra acetic acid in niosomes and their antimicrobial activity. J Sci Res 4:457–465

    Article  CAS  Google Scholar 

  13. Leive L (1965) Release of lipopolysaccharide by EDTA treatment of E. coli. Biochem Biophys Res Commun 21:290–296

    Article  CAS  PubMed  Google Scholar 

  14. Noginov MA, Zhu G, Bahoura M, Adegoke J, Small C, Ritzo BA, Shalaev VM (2007) The effect of gain and absorption on surface plasmons in metal nanoparticles. Appl Phys B 86(3):455–460

    Article  CAS  Google Scholar 

  15. Pastoriza-Santos I, Liz-Marzán LM (1999) Formation and stabilization of silver nanoparticles through reduction by N,N-dimethylformamide. Langmuir 15(4):948–951

    Article  CAS  Google Scholar 

  16. Randall CP, Oyama LB, Bostock JM, Chopra I, O’Neill AJ (2013) The silver cation (Ag+): anti staphylococcal activity, mode of action and resistance studies. J Antimicrob Chemother 68(1):131–138

    Article  CAS  PubMed  Google Scholar 

  17. Schved F, Henis Y, Juven BJ (1994) Response of spheroplasts and chelator-permeabilized cells of gram-negative bacteria to the action of the bacteriocins pediocin SJ-1 and nisin. Int J Food Microbiol 21:305–314

    Article  CAS  PubMed  Google Scholar 

  18. Sichani GN, Morshed M, Amirnasr M, Abedi D (2010) In situ preparation, electrospinning, and characterization of polyacrylonitrile nanofibers containing silver nanoparticles. J Appl Polym Sci 116(2):1021–1029

    CAS  Google Scholar 

  19. Singh AP, Prabha V, Rishi P (2013) Value addition in the efficacy of conventional antibiotics by Nisin against Salmonella. PLoS One 8(10):e76844

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  20. Wang Y, Yang Q, Shan G, Wang C, Du J, Wang S, Wei Y (2005) Preparation of silver nanoparticles dispersed in polyacrylonitrile nanofiber film spun by electrospinning. Mater Lett 59(24):3046–3049

    Article  CAS  Google Scholar 

  21. Wu J, Zheng Y, Song W, Luan J, Wen X, Wu Z, Guo S (2014) In situ synthesis of silver-nanoparticles/bacterial cellulose composites for slow-released antimicrobial wound dressing. Carbohydr Polym 102:762–771

    Article  CAS  PubMed  Google Scholar 

  22. Zacharof MP, Lovitt RW (2012) Bacteriocins produced by lactic acid bacteria a review article. APCBEE Procedia 2:50–56

    Article  CAS  Google Scholar 

Download references

Acknowledgments

Ahire JJ is grateful to the Claude Leon Foundation, Cape Town, South Africa, for a postdoctoral fellowship.

Author information

Authors and Affiliations

  1. Department of Microbiology, University of Stellenbosch, Matieland, Stellenbosch, 7602, South Africa

    Jayesh J. Ahire, Deon P. Neveling & Leon M. T. Dicks

Authors
  1. Jayesh J. Ahire
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Deon P. Neveling
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Leon M. T. Dicks
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Leon M. T. Dicks.

Electronic supplementary material

Below is the link to the electronic supplementary material.

284_2015_813_MOESM1_ESM.pptx

Supplementary material 1 Energy-dispersive X-ray (EDX) analysis of (a) silver nanofibers (SF) and silver plus nisin nanofibers (SNF). C: carbon, O: oxygen and Ag: silver (PPTX 97 kb)

284_2015_813_MOESM2_ESM.pptx

Supplementary material 2 Atomic force microscopy (AFM) images of (a) control nanofibers (CF), (b) silver nanofibers (SF), (c) nisin nanofibers (NF), and silver plus nisin nanofibers (SNF) (PPTX 303 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ahire, J.J., Neveling, D.P. & Dicks, L.M.T. Co-spinning of Silver Nanoparticles with Nisin Increases the Antimicrobial Spectrum of PDLLA: PEO Nanofibers. Curr Microbiol 71, 24–30 (2015). https://doi.org/10.1007/s00284-015-0813-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00284-015-0813-y

Keywords

Search

Navigation