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Pedobacter hainanensis sp. nov., Isolated from Seaside Soil

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Abstract

A Gram-negative, aerobic, rod-shaped, motile, non-spore-forming bacterial strain, designated 13-QT, was isolated from seaside soil under the stacks of the red algae in Hainan province in China. Identification was carried out on the basis of polyphasic taxonomy. Phylogenetic analysis of 16S rRNA gene sequences showed that strain 13-QT belonged to the genus Pedobacter, and the highest similarity was 94.4 % with Pedobacter terricola KCTC 12876T. Strain 13-QT was able to grow at 10–40 °C, in pH 5.0–10.0, in the presence of 0–2.0 % NaCl. The major fatty acids were iso-C15:0 (40.4 %), summed feature 3 (comprising iso-C15:0 2-OH and/or C16:1 ω7c) (18.9 %) and iso-C17:0 3-OH (18.4 %). The predominant menaquinone was MK-7. The G+C content of the genomic DNA was 42.7 mol%. Strain 13-QT could be distinguished from the nearest phylogenetic neighbors by various chemotaxonomic and phenotypic properties. The results of the polyphasic analyses suggested that strain 13-QT should be considered to represent a novel species of the genus Pedobacter, for which the name Pedobacter hainanensis sp. nov. is proposed. The type strain is 13-QT (=CCTCC AB 2012076T = NRRL B-59850T).

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Abbreviations

LB:

Luria-Bertani agar

MA:

Marine agar 2216

NA:

Nutrient agar

TSA:

Trypticase soy agar

TSB:

Trypticase soy broth

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Acknowledgments

The work was supported by Hi-Tech Research and Development Program of China (Grant No. 2011AA090704, No. 2011AA10A205), National Department Public Benefit Research Foundation of China (Grant No. 200903052), and National Basic Research Program of China (Grant No. 2011CB200906).

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Authors and Affiliations

  1. Liaoning Provincial Key Laboratory of Carbohydrates, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, People’s Republic of China

    Yan-yu Meng, Yun-bin Fu, Meng-yu Wang, Yong Zhao, Qi-shun Liu & Yu-guang Du

  2. Graduate University of the Chinese Academy of Sciences, Beijing, 100049, People’s Republic of China

    Yan-yu Meng, Yun-bin Fu, Meng-yu Wang, Yong Zhao, Qi-shun Liu & Yu-guang Du

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  1. Yan-yu Meng
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  2. Yun-bin Fu
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  3. Meng-yu Wang
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  4. Yong Zhao
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  6. Yu-guang Du
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Corresponding author

Correspondence to Yu-guang Du.

Additional information

The GenBank/EMBL/DDBJ accession number for 16S rRNA gene sequence of strain 13-QT is JQ 083404.

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Meng, Yy., Fu, Yb., Wang, My. et al. Pedobacter hainanensis sp. nov., Isolated from Seaside Soil. Curr Microbiol 66, 487–492 (2013). https://doi.org/10.1007/s00284-013-0305-x

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  • DOI: https://doi.org/10.1007/s00284-013-0305-x

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