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Fe and P Solubilization Under Limiting Conditions by Bacteria Isolated from Carex kobomugi Roots at the Hasaki Coast

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Abstract

Our objective was simply to report a sedge species, Carex kobomugi Ohwi that has beneficial bacterial associations under low Fe and P conditions of the Hasaki coast, Japan. C. kobomugi is the dominant species in our study area and grows closest to the sea. C. kobomugi showed higher Fe and P content, while these nutrients were less available under alkaline root-zone soil. Within the roots, mycorrhizal fungal colonization was absent, and endophytic fungal colonization was low. On the contrary, endophytic bacteria (e.g. Bacillus sp., Streptomyces luteogriseus, and Pseudomonas fluorescens) were isolated, which exhibited both siderophore production and inorganic phosphate solubilization under Fe or P limited conditions. Our results suggest that colonization of root tissue by these bacteria contribute to the Fe and P uptakes by C. kobomugi by increasing availability in the soil.

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Abbreviations

AMF:

Arbuscular mycorrhizal fungi

PGPB:

Plant growth-promoting bacteria

EC:

Electrical conductivity

ICP-AES:

Inductively coupled argon plasma emission spectrophotometry

CEC:

Cation exchange capacity

NB:

Nutrient broth

YGP:

Yeast–glucose–polypeptone

CAS:

Chrome azurol S

DCP:

Dicalcium phosphate

PSAI:

Phosphate-solubilizing activity index

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Acknowledgments

The authors are grateful to Dr. H. Ishimoto for conducting the CAS assay, and the Chemical Analysis Division, Research Facility Center for Science and Technology, University of Tsukuba, for measuring the ICP.

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Authors and Affiliations

  1. Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki, Japan

    Hiroaki Matsuoka, Katsuichiro Kobayashi & Keiko Yamaji

  2. Geoscience Research Laboratory, Co., Ltd, Toriumi Bldg. 2F, 1794 Kamiwada, Yamato, Kanagawa, Japan

    Masaru Akiyama

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  1. Hiroaki Matsuoka
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  2. Masaru Akiyama
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Correspondence to Keiko Yamaji.

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Matsuoka, H., Akiyama, M., Kobayashi, K. et al. Fe and P Solubilization Under Limiting Conditions by Bacteria Isolated from Carex kobomugi Roots at the Hasaki Coast. Curr Microbiol 66, 314–321 (2013). https://doi.org/10.1007/s00284-012-0276-3

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