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A Novel Antifungal Pseudomonas fluorescens Isolated from Potato Soils in Greenland

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Abstract

A rhizobacterium with high antifungal activity was isolated from a potato field at Inneruulalik, South Greenland. Phylogenetic analysis based on multi locus sequence typing showed that the bacterium was affiliated with strains of Pseudomonas fluorescens. The bacterium, denoted as Pseudomonas fluorescens In5, inhibited in vitro a broad range of phytopathogenic fungi, and the antifungal activity increased with decreasing temperature. Microcosm experiments demonstrated that P. fluorescens In5 protected tomato seedlings from Rhizoctonia solani. Transposon mutagenesis showed that the major cause for the antifungal activity of P. fluorescens In5 was a novel non-ribosomal peptide synthase (NRPS) gene. In addition, transposon mutagenesis showed that P. fluorescens In5 also contained a putative quinoprotein glucose dehydrogenase gene, which was involved in growth inhibition of phytopathogenic fungi. Although P. fluorescens In5 contained the capacity to synthesize hydrogen cyanide, β-1,3-glucanase, protease, and chitinase, these did not seem to play a role in the in vitro and microcosm antifungal assays.

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Acknowledgments

We thank chief counselor Kenneth Høegh, Qaqortoq, Greenland, for support during sampling of material and research in Greenland. Anders Priemé and Martin Asser Hansen are thanked for establishing the near-whole genome sequence of strain In5. Eigil de Neergaard, Danish Plant Directorate and Lisa Munk, University of Copenhagen, are acknowledged for communicating unpublished data on the occurrence of fungal pathogens in Greenland. Referring to the Convention on Biological Diversity, we thank the Greenland Home Rule for permission to sample bacteria in South Greenland. This work was funded in part by the Commission for Scientific Research in Greenland.

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  1. Department of Agriculture and Ecology, Faculty of Life Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg C, Denmark

    Charlotte Frydenlund Michelsen & Peter Stougaard

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  1. Charlotte Frydenlund Michelsen
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  2. Peter Stougaard
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Correspondence to Peter Stougaard.

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Michelsen, C.F., Stougaard, P. A Novel Antifungal Pseudomonas fluorescens Isolated from Potato Soils in Greenland. Curr Microbiol 62, 1185–1192 (2011). https://doi.org/10.1007/s00284-010-9846-4

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