Abstract
The fastidious bacterium Xylella fastidiosa is associated with important crop diseases worldwide. We have recently shown that X. fastidiosa is a peculiar organism having unusually low values of gene codon bias throughout its genome and, unexpectedly, in the group of the most abundant proteins. Here, we hypothesized that the lack of codon usage optimization in X. fastidiosa would incapacitate this organism to undergo quick and massive changes in protein expression as occurs in a classical stress response. Proteomic analysis of the response to heat stress in X. fastidiosa revealed that no changes in protein expression can be detected. Moreover, stress-inducible proteins identified in the closely related citrus pathogen Xanthomonas axonopodis pv citri were found to be constitutively expressed in X. fastidiosa. These proteins have extremely high codon bias values in the X. citri and other well-studied organisms, but low values in X. fastidiosa. Because biased codon usage is well known to correlate to the rate of protein synthesis, we speculate that the peculiar codon bias distribution in X. fastidiosa is related to the absence of a classical stress response, and, probably, alternative strategies for survival of X. fastidiosa under stressfull conditions.
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Literature Cited
Chang CJ, Garner M, Zreik L, et al. (1993) Culture and serological detection of xylem-limited bacterium causing citrus variegated chlorosis and its identification as a strain of Xylella fastidiosa. Curr Microbiol 27:137–142
Chen J, Roberts PD, Gabriel DW (1994) Effects of a virulence locus from Xanthomonas campestris 582T on pathovar status and ability to elicit blight symptoms on crucifers. Phytopathology 84:1458–1456
Amaro AA, Maia ML, Gonzales MA (1998) In: Donadio LC, Moreira CS (eds) Citrus variegated chlorosis, Est. Exp. Citricultura, Bebedouro, SP, Brazil; pp. 123–139
Simpson AJ, Reinach FC, Arruda P, et al. (2000) The genome sequence of the plant pathogen Xylella fastidiosa. Nature 406(6792):151–157
Purcell AH, Hopkins DL (1996) Fastidious xylem-limited bacterial plant pathogens. Ann Rev Phytopathol 34:131–151
Hopkins DL (1989) Xylella fastidiosa: a xylem-limited bacterial pathogen of plants. Ann Rev Phytopathol 27:271–290
da Silva AC, Ferro JA, Reinach FC, et al. (2002) Comparison of the genomes of two Xanthomonas pathogens with differing host specificities. Nature 417(6887):459–463
Smolka MB, Martins D, Winck FV, et al. (2003) Proteome analysis of the plant pathogen Xylella fastidiosa reveals major cellular and extracellular proteins and a peculiar codon bias distribution. Proteomics 3:224–237
Bukau B, Horwich AL (1998) The Hsp70 and Hsp60 chaperone machines. Cell 92(3):351–366
Lawrence JG, Ochman H (1997) Amelioration of bacterial genomes: rates of change and exchange. J Mol Evol 44(4):383–397
Ikemura T (1985) Codon usage and tRNA content in unicellular and multicellular organisms. Mol Biol Evol 2(1):13–34
Grantham R, Gautier C, Gouy M, et al. (1981) Codon catalog usage is a genome strategy modulated for gene expressivity. Nucleic Acids Res 9(1):43–74
Munchbach M, Dainese P, Staudenmann W, et al. (1999) Proteome analysis of heat shock protein expression in Bradyrhizobium japonicum. Eur J Biochem 264(1):39–48
Bennetzen JL, Hall BD (1982) Codon selection in yeast. J Biol Chem 257(6):3026–3031
Veinger L, Diamant S, Buchner J, Goloubinoff P (1998) The small heat-shock protein IbpB from Escherichia coli stabilizes stress-denatured proteins for subsequent refolding by a multichaperone network. J Biol Chem 273:11032–11037
Ehrnsperger M, Graber S, Gaestel M, Buchner J (1997) Binding of non-native protein to Hsp25 during heat shock creates a reservoir of folding intermediates for reactivation. EMBO J 16(2):221–229
Acknowledgments
We would like to thank FAPESP (Fundação de Amparo a Pesquisa do Estado de São Paulo) for support of this work and Prof. Phillip Cash from the University of Aberdeen, Scotland, for the valuable suggestions and corrections of the manuscript.
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An erratum to this article is available at http://dx.doi.org/10.1007/s00284-009-9441-8.
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Martins, D., Astua-Monge, G., Coletta-Filho, H.D. et al. Absence of Classical Heat Shock Response in the Citrus Pathogen Xylella fastidiosa . Curr Microbiol 54, 119–123 (2007). https://doi.org/10.1007/s00284-006-0215-2
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DOI: https://doi.org/10.1007/s00284-006-0215-2