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Bio-Revitalization: Effects of NASHA on Genes Involving Tissue Remodeling

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  • Aesthetic
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Abstract

Background

The “non-animal stabilized hyaluronic acid” (NASHA) is a widely used product in bio-revitalization injective procedures in esthetic medicine. The present research aimed to quantitatively evaluate the therapeutic effect of one of the more used bio-revitalization products on cultured dermal fibroblasts. RT-PCR was used for gene expression profiling of some proteins known to be relevant in skin homeostasis.

Methods

Human dermal fibroblasts were seeded on a culture medium enriched with a product for dermal bio-revitalization, consisting of stabilized hyaluronic acid gel 20 mg/ml. After 24, 48, and 72 h of exposure, the cDNA was amplified by real-time PCR. Gene expression was quantified with the delta/delta calculation method.

Results

In this study, the gene of metalloproteinase (MMP)-13 is strongly expressed after NASHA incubation. The MMP-2 encoding gene instead is less expressed, but both evidence the same temporal trend, being progressively up-regulated after 24 and 48 h, thereafter the expression decreases, whereas MMP-3 maintains the same up-regulation at 72 h. Hyaluronan synthase 1 and desmoplakin are progressively up-regulated and increase at 24, 48, and 72 h. Hyaluronidase 1 and neutrophil elastase genes are overexpressed, but at 72 h they both exhibit the same behavior as the other degradative enzymes MMP-13 and MMP-2.

Conclusions

Skin bio-revitalization by injecting the tested NASHA gel produces an enhancement in the expression of some genes involved in extracellular matrix degradation and organization. In this study, a time-dependent behavior, different for genes encoding degradative compared to synthesis proteins, was demonstrated.

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Conflict of interest

The authors declare that they have no conflict of interest.

Funding

The products used in the present study were bought independently by the authors and no founds were received from the company. The proprietary name, also known as brand name or trade name, was used for clarity and should not be viewed as an endorsement by the authors of a specific product.

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Authors and Affiliations

  1. Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy

    A. Avantaggiato & F. Carinci

  2. Department of Experimental, Diagnostic and Specialty Medicine, University of Bologna, Bologna, Italy

    A. Girardi & A. Palmieri

  3. Rome, Italy

    M. Pascali

Authors
  1. A. Avantaggiato
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  2. A. Girardi
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  3. A. Palmieri
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  4. M. Pascali
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  5. F. Carinci
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Corresponding author

Correspondence to A. Palmieri.

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Avantaggiato, A., Girardi, A., Palmieri, A. et al. Bio-Revitalization: Effects of NASHA on Genes Involving Tissue Remodeling. Aesth Plast Surg 39, 459–464 (2015). https://doi.org/10.1007/s00266-015-0514-8

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  • DOI: https://doi.org/10.1007/s00266-015-0514-8

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