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Effects of montelukast on tendon healing in a murine model

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Abstract

Background

Tendon injury induces a local inflammatory response characterized by the production of pro-inflammatory cytokines. The aim of this study is to investigate the effects of montelukast sodium on the healing of tendons through histological and biomechanical evaluations.

Methods

Forty-eight female Wistar albino rats were randomly assigned to an experimental group that received montelukast sodium (n = 24) and a control group (n = 24) that did not. Tendon injury was created in the Achilles tendon. The experimental group was injected intraperitoneally (IP) with 1 ml of 1 mg/kg montelukast sodium solution once a day prior to the surgery and during the experimental research. The control group was injected with saline solution. Two weeks later, eight rats in each group underwent a histological evaluation. In the fourth week, eight rats underwent a histological evaluation and the other eight rats went through a biomechanical evaluation.

Results

Based on the histological evaluation in the second week, it was observed that the severity of the inflammation was less in the experimental group that received montelukast sodium (p < 0.05). In terms of the formation of collagen, no significant difference was observed between the groups in the second and fourth weeks. Tendon breaking loads were 33.2 ± 10.95 and 38.8 ± 10.90 N for the montelukast group and the control group, respectively. However, the difference between the groups was found to be statistically insignificant (p > 0.05).

Conclusions

There was no negative effect on the healing of tendons due to injection of montelukast sodium. In addition, observing less inflammation in the experimental group in the earlier phase suggests that montelukast sodium may help in preventing tendon adhesion after reconstructive treatment.

Level of Evidence: Not ratable.

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Authors and Affiliations

  1. Aesthetic, Plastic and Reconstructive Surgery Clinic, Dr. Lütfi Kırdar Kartal Education and Research Hospital, Istanbul, Turkey

    Kaan Gideroğlu

  2. Department of Orthopaedic Surgery, Abant İzzet Baysal University, Bolu, Turkey

    Hüsamettin Çakıcı & Kutay E. Özturan

  3. Department of Orthopaedic Surgery, Mustafa Kemal University, Antakya, Turkey

    Onur Hapa

  4. Faculty of Mechanical Engineering, Istanbul Technical University, Istanbul, Turkey

    Ergun Bozdağ

  5. Department of Pathology, Abant İzzet Baysal University, Bolu, Turkey

    Fahri Yılmaz

  6. Department of Aesthetic, Plastic and Reconstructive Surgery, Faculty of Medicine, Harran University, 63100, Şanlıurfa, Turkey

    İbrahim Sağlam

Authors
  1. Kaan Gideroğlu
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  2. Hüsamettin Çakıcı
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  3. Onur Hapa
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  4. Kutay E. Özturan
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  5. Ergun Bozdağ
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  6. Fahri Yılmaz
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  7. İbrahim Sağlam
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Corresponding author

Correspondence to İbrahim Sağlam.

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Conflict of interest

Kaan Gideroğlu, Hüsamettin Çakıcı, Onur Hapa, Kutay E. Özturan, Ergun Bozdağ, Fahri Yılmaz, and İbrahim Sağlam declare that they have no conflict of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted. The experimental design and all the procedures were approved by the Abant İzzet Baysal University Ethics Committee.

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Gideroğlu, K., Çakıcı, H., Hapa, O. et al. Effects of montelukast on tendon healing in a murine model. Eur J Plast Surg 40, 171–176 (2017). https://doi.org/10.1007/s00238-017-1283-1

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  • DOI: https://doi.org/10.1007/s00238-017-1283-1

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