Abstract
Genetic factors are thought to maintain bone mass in socioeconomically disadvantaged black South Africans. We compared bone mass between environmentally disadvantaged black and advantaged white children and their parents, after determining the most appropriate method by which to correct bone mineral content (BMC) for size. We collected data from 419 healthy black and white children of mean age 10.6 years (range 10.0–10.9), 406 biological mothers, and 100 biological fathers. Whole-body, femoral neck, lumbar spine, and mid- and distal one-third of radius bone area (BA) and BMC were measured by dual-energy X-ray absorptiometry. Power coefficients (PCs) were calculated from the linear-regression analyses of ln(BMC) on ln(BA) and used to correct site-specific BMC for bone size differences. Heritability (½h2, %) by maternal and paternal descent was estimated by regressing children’s Z scores on parents’ Z scores. Correcting BMC for height, weight, and BAPC accounted for the greatest variance of BMC at all skeletal sites. In so doing, BMC in blacks was up to 2.6 times greater at the femoral neck and lumbar spine. Maternal and paternal heritability was estimated to be ~30% in both black and white subjects. These results may in part explain the lower prevalence of fragility fractures at the hip in black South African children when compared to whites. Heritability was comparable between environmentally disadvantaged black and advantaged white South African children and similar to that reported for Caucasians in other parts of the world.
Similar content being viewed by others
References
Cameron N (1996) Birth to ten: the first five years. Leech 65:42–44
MacKeown J, Cleaton-Jones P, Norris S (2003) Nutrient intake among a longitudinal group of urban black South African children at four interceptions between 1995 and 2000. Nutr Res 23:185–197
McVeigh J, Norris S, de Wet T (2004) The relationship between socioeconomic status and physical activity patterns in South African children. Acta Paediatr 93:1–7
McVeigh J, Norris S, Cameron N, Pettifor J (2004) Associations between physical activity and bone mass in black and white South African children at age 9 yr. J Appl Physiol 97:1006–1012
Vidulich L, Norris S, Cameron N, Pettifor J (2006) Differences in bone size and bone mass between black and white 10-year-old South African children. Osteoporos Int 17:433–440
Daniels E, Pettifor J, Schnitzler C, Russell S, Patel D (1995) Ethnic differences in bone density in female South African nurses. J Bone Miner Res 10:359–367
Solomon L (1968) Osteoporosis and fracture of the femoral neck in South African blacks. J Bone Joint Surg 50:2–13
Dent C, Engelbrecht H, Godfrey R (1968) Osteoporosis of lumbar vertebrae and calcification of abdominal aorta in women living in Durban. Br Med J 4:76–79
Thandrayen K, Norris S, Pettifor J (2009) Fracture rates in urban South African children of different ethnic origins: the Birth to Twenty Cohort. Osteoporos Int 20:47–52
Picard D, Imbach A, Couturier M, Lepage R, Picard M (2001) Familial resemblance of bone mineral density between females 18 years and older and their mothers. Can J Public Health 92:353–358
Matkovic V, Fontana D, Tominac C, Goel P, Chestnut C III (1990) Factors that influence peak bone mass formation: a study of calcium balance and the inheritance of bone mass in adolescent females. Am J Clin Nutr 52:878–888
Jones G, Nguyen T (2000) Associations between maternal peak bone mass and bone mass in prepubertal male and female children. J Bone Miner Res 15:1998–2004
Binkovitz L, Henwood M (2007) Pediatric DXA: technique and interpretation. Pediatr Radiol 37:21–31
Kalkwarf H, Zemel B, Gilsanz V, Lappe J, Horlick M, Oberfield S, Mahboubi S, Fan B, Frederick M, Winer K, Shepperd J (2007) The Bone Mineral Density in Childhood Study (BMDCS): bone mineral content and density according to age, sex and race. J Clin Endocrinol Metab 92:2087–2099
Mølgaard C, Thomsen B, Prentice A, Cole T, Michaelsen K (1997) Whole body bone mineral content in healthy children and adolescents. Arch Dis Child 76:9–15
Gilsanz V, Wren T (2007) Assessment of bone acquisition in childhood and adolescence. Pediatrics 119:S145–S149
Bachrach L (2000) Dual-energy X-ray absorptiometry (DEXA) measurements of bone density and body composition: promise and pitfalls. J Pediatr Endocrinol Metab 2:983–988
Katzman D, Bachrach L, Carter D, Marcus R (1991) Clinical and anthropometric correlates of bone mineral acquisition in healthy adolescent girls. J Clin Endocrinol Metab 73:1332–1339
Carter D, Bouxsein M, Marcus R (1992) New approaches for interpreting projected bone densitometry data. J Bone Miner Res 7:137–145
Kröger H, Kontaniemi A, Vainio P, Alhava E (1992) Bone densitometry of the spine and femur in children by dual-energy X-ray absorptiometry. Bone Miner 17:75–85
Lu P, Cowell C, Lloyd-Jones S, Brody J, Howman-Giles R (1996) Volumetric bone density in normal subjects aged 5–27 years. J Clin Endocrinol Metab 81:1586–1590
Prentice A, Parsons T, Cole T (1994) Uncritical use of bone mineral density in absorptiometry may lead to size-related artefacts in the identification of bone mineral determinants. Am J Clin Nutr 60:837–842
Lohman T, Roche A, Martorell R (1991) Anthropometric standardization reference manual. Human Kinetics, Champaign
Marshall W, Tanner JM (1969) Variations in the pattern of pubertal changes in girls. Arch Dis Child 44:291–303
Marshall W, Tanner J (1970) Variations in the pattern of pubertal changes in boys. Arch Dis Child 45:13–23
Tanner J, Whitehouse R, Cameron N (1983) Assessment of skeletal maturity and prediction of adult height. Academic Press, London
Bradshaw D, Steyn K (2001) Poverty and chronic diseases in South Africa. Technical report. Medical Research Council of South Africa, Cape Town
Beck T, Ruff C, Warden K, Scott W, Rao G (1990) Predicting femoral neck strength from bone mineral data. A structural approach. Invest Radiol 25:6–18
Beck T (2003) Measuring the structural strength of bones with dual-energy X-ray absorptiometry: principles, technical limitations, and future possibilities. Osteoporos Int 14(5):S81–S88
Magarey A, Boulton T, Chatterton B, Schultz C, Nordin B (1999) Familial and environmental influences on bone growth from 11–17 years of age. Acta Pediatr 88:1204–1210
Nordström P, Lorentzon M (1999) Influence of heredity and environment on bone density in adolescent boys: a parent–offspring study. Osteoporos Int 10:271–277
Nyati L, Norris S, Cameron N, Pettifor J (2006) Effect of ethnicity and sex on the growth of the axial and appendicular skeleton of children living in a developing country. Am J Phys Anthropol 131:135–141
Daniels E, Pettifor J, Schnitzler C, Moodley G, Zachen D (1997) Differences in mineral homeostasis, volumetric bone mass and femoral neck axis length in black and white South African women. Osteoporos Int 7:105–112
Solomon L (1979) Bone density in ageing Caucasian and African populations. Lancet 2:1326–1330
Nelson D, Pettifor J, Barondess D, Cody D, Uusi-Rasi K, Beck T (2004) Comparison of cross-sectional geometry of the proximal femur in white and black women from Detroit and Johannesburg. J Bone Miner Res 19:560–565
Nelson D, Barondess D, Hendrix S, Beck T (2000) Cross-sectional geometry, bone strength and bone mass in the proximal femur in African-American and white postmenopausal women. J Bone Miner Res 15:1992–1997
Seeman E (1997) From density to structure: growing up and growing old on the surfaces of bones. J Bone Miner Res 12:509–521
Schnitzler C, Pettifor J, Mesquita J, Bird M, Schnaid E, Smyth A (1990) Histomorphometry of iliac crest bone in 346 normal black and white South African adults. Bone Miner 10:183–189
Schnitzler C, Mesquita J, Pettifor J (2009) Cortical bone development in black and white South African children: iliac crest histomorphometry. Bone 44:603–611
Schnitzler C, Mesquita J (2006) Cortical bone histomorphometry of the iliac crest in normal black and white South African adults. Calcif Tissue Int 79:373–382
Micklesfield L, Norris S, van der Merwe L, Pettifor J (2009) Comparison of site specific bone mass indices in South African children of different ethnic groups. Calcif Tissue Int 85:317–325
Wang X, Wang Q, Ghasem-Zadeh A, Evans A, McLeod C, Iuliano-Burns S, Seeman E (2009) Differences in macro- and microarchitecture of the appendicular skeleton in young Chinese and white women. J Bone Miner Res 24:1946–1952
Gilsanz V, Skaggs D, Kovanlikaya A, Sayre J, Loro M, Kaufman F, Korenman S (1998) Differential effect of race on the axial and appendicular skeletons of children. J Clin Endocrinol Metab 83:1420–1427
Wang X, Kammerer C, Wheeler V, Patrick A, Bunker C, Zmuda J (2007) Genetic and environmental determinants of volumetric and areal BMD in multi-generational families of African ancestry: the Tobago Family Health Study. J Bone Miner Res 22:527–536
Duren D, Sherwood R, Choh A, Czerwinski S, Chumlea W, Lee M, Sun S, Demerath E, Siervogel R, Towne B (2007) Quantitative genetics of cortical bone mass in health 10-year-old children from the Fels Longitudinal Study. Bone 40:464–470
Roberts D, Billewicz W, McGregor I (1978) Heritability of stature in a West African population. Ann Hum Genet 42:15–24
Blain H, Vuillemin A, Guillemin F, Jouanny P, Jeandel C, Le Bihan E (2006) Lean mass plays a gender-specific role in familial resemblance for femoral neck bone mineral density in adult subjects. Osteoporos Int 17:897–907
Acknowledgments
We acknowledge the contributions of Saeeda Mohamed and Thabile Sibiya for their DXA measurements. The Wellcome Trust of the United Kingdom and the Medical Research Council of South Africa funded this research.
Author information
Authors and Affiliations
Corresponding author
Additional information
The authors have stated that they have no conflict of interest.
Rights and permissions
About this article
Cite this article
Vidulich, L., Norris, S.A., Cameron, N. et al. Bone Mass and Bone Size in Pre- or Early Pubertal 10-Year-Old Black and White South African Children and Their Parents. Calcif Tissue Int 88, 281–293 (2011). https://doi.org/10.1007/s00223-011-9460-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00223-011-9460-x