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Fractionation and isolation of polyphenols from Aronia melanocarpa by countercurrent and membrane chromatography

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Abstract

In the current study, the fractionation and isolation of polyphenols from Aronia melanocarpa has been performed with two different chromatographic techniques on a large scale. On the one hand, the fractionation of polyphenols such as anthocyanins, phenolic acids, quercetin–glycosides and flavanons from A. melanocarpa pomace was done by high-speed countercurrent chromatography and low-speed rotary countercurrent chromatography. On the other hand, the preseparation of A. melanocarpa extracts from pomace and juice in an anthocyanin and a co-pigment fraction was carried out by membrane chromatography after removing the polymeric procyanidins by precipitation with ethanol. Afterward, the separation and isolation of anthocyanins and co-pigments were done by preparative countercurrent chromatography. Purity control and identification of the isolated compounds were made by HPLC–PDA, HPLC–ESI-MSn and 1H- as well as 13C-NMR spectroscopy. Various compounds, e.g., chlorogenic acids, isorhamnetin-, apigenin-, luteolin- and taxifolin-derivatives, are described for A. melanocarpa for the first time.

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Abbreviations

HPLC:

High performance liquid chromatography

HSCCC:

High-speed countercurrent chromatography

LSRCCC:

Low-speed rotary countercurrent chromatography

HPCCC:

High-performance countercurrent chromatography

TFA:

Trifluoroacetic acid

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Acknowledgements

We are grateful for the technical assistance of Janina Westphal and Josefine Ostberg. The authors thank the German Federal Ministry of Education and Research (BMBF—Bundesministerium für Bildung and Forschung) for financial support of the joint project “Dietary procyanidins” (Grant 0313828C). A. melanocarpa samples were kindly provided by Melanie Stürtz (Symrise, Holzminden, Germany) and Kelterei Walther (Dresden, Germany).

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Authors and Affiliations

  1. Institute of Food Chemistry, Technische Universität Braunschweig, Schleinitzstrasse 20, 38106, Braunschweig, Germany

    Tuba Esatbeyoglu, Miriam Rodríguez-Werner & Peter Winterhalter

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  1. Tuba Esatbeyoglu
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  2. Miriam Rodríguez-Werner
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  3. Peter Winterhalter
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Correspondence to Tuba Esatbeyoglu.

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Esatbeyoglu, T., Rodríguez-Werner, M. & Winterhalter, P. Fractionation and isolation of polyphenols from Aronia melanocarpa by countercurrent and membrane chromatography. Eur Food Res Technol 243, 1261–1275 (2017). https://doi.org/10.1007/s00217-016-2837-3

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  • DOI: https://doi.org/10.1007/s00217-016-2837-3

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