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Setup of a procedure for cider proteins recovery and quantification

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Abstract

Cider contains low amount of proteins that, nonetheless, can affect its stability, foam formation and potential allergenicity. At present, scarce information is available on cider proteins, probably due to the lack of methods for their recovery and analysis. The aim of the present study was to set up a method for recovering and quantifying cider proteins. To this purpose, the proteins from 13 Italian commercial ciders were recovered by dialysis, gel filtration, trichloroacetic acid/acetone (TCA/acetone) and potassium dodecyl sulfate (KDS) precipitation. The protein content of the samples was then determined by bicinchoninic acid (BCA), Bradford and o-phthaldialdehyde (OPA) assays. The results were compared to quantitative data obtained by densitometry of electrophoretic gels. The most reliable protocol resulted in the KDS method followed by OPA assay. KDS, in addition, allowed also to separate proteins from glycocompounds. KDS/OPA is the method of choice for cider proteins precipitation and quantification.

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Acknowledgments

We thank L.M. di Maria Lucia Melchiori and C. s.n.c. and Maley s.r.l. for having kindly supplied cider samples.

Funding

This work was supported by University of Padova (Grant Number CPDA148173 and 60A08-1950/15).

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Authors and Affiliations

  1. Department of Agronomy Food Natural Resources Animals and Environment, University of Padova, Agripolis, Viale dell’Università, 16, 35020, Legnaro, Padova, Italy

    Federica Mainente & Gabriella Pasini

  2. Department of Biotechnology, University of Verona, Verona, Italy

    Federica Mainente, Corrado Rizzi, Gianni Zoccatelli, Roberto Chignola & Barbara Simonato

Authors
  1. Federica Mainente
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  2. Corrado Rizzi
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  3. Gianni Zoccatelli
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  4. Roberto Chignola
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  5. Barbara Simonato
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  6. Gabriella Pasini
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Correspondence to Federica Mainente.

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Mainente, F., Rizzi, C., Zoccatelli, G. et al. Setup of a procedure for cider proteins recovery and quantification. Eur Food Res Technol 242, 1803–1811 (2016). https://doi.org/10.1007/s00217-016-2724-y

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  • DOI: https://doi.org/10.1007/s00217-016-2724-y

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