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Postmortem changes in sarcoplasmic proteins associated with color stability in lamb muscle analyzed by proteomics

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Abstract

The aim of this research was to correlate the postmortem changes of sarcoplasmic proteins with instrumental color and various biochemical parameters relating to color attributes. Two-dimensional electrophoresis was used to investigate sarcoplasmic protein changes in cardiac muscle during refrigerated storage. Eleven differentially abundant protein spots were identified between storage time at day 1 and day 5 (P < 0.05). The functional category of proteins consisted of metabolic enzymes, binding proteins, chaperone proteins, and antioxidant proteins. These proteins demonstrated positive correlation with a*-value (creatine kinase M-type and malate dehydrogenase), metmyoglobin-reducing activity (aconitate hydratase, creatine kinase, and thioredoxin-dependent peroxide reductase), tissue oxygen consumption rate (creatine kinase and malate dehydrogenase), and mitochondrial respiration control ratio (thioredoxin-dependent peroxide reductase and mitochondrial superoxide dismutase). The protein patterns were likely to have impact on postmortem metabolism, thereby protecting muscle against discoloration.

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Acknowledgments

We gratefully acknowledge the financial support by Nature Science Foundation of Hebei province China (Project No: C2015208052), Science and technology research project of Hebei Education Department (Project No: QN2015120) and the Ministry of Agriculture of China (China Agriculture Research System-39, CARS-39).

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Authors and Affiliations

  1. College of Bioscience and Bioengineering, Hebei University of Science and Technology, No.26 Yuxiang Street, Yuhua District, Shijiazhuang, 050000, Hebei, People’s Republic of China

    Xiaoguang Gao

  2. College of Food Science and Nutritional Engineering, China Agricultural University, No.17 Qinghua East Road, Haidian District, Beijing, 100083, People’s Republic of China

    Xiaoguang Gao, Wei Wu, Changwei Ma, Xingmin Li & Ruitong Dai

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  1. Xiaoguang Gao
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  2. Wei Wu
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Correspondence to Ruitong Dai.

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All institutional and national guidelines for the care and use of laboratory animals were followed.

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Gao, X., Wu, W., Ma, C. et al. Postmortem changes in sarcoplasmic proteins associated with color stability in lamb muscle analyzed by proteomics. Eur Food Res Technol 242, 527–535 (2016). https://doi.org/10.1007/s00217-015-2563-2

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  • DOI: https://doi.org/10.1007/s00217-015-2563-2

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