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Comparative study of phenolic profile of fruit and juice samples of a progeny of ‘Meana’ × ‘Florina’ from an Asturian cider apple breeding program

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Abstract

In the present work, a comparative study of the detailed phenolic profile of fruit and juice samples from an Asturian cider apple breeding program was carried out. To achieve this, a fast, selective and sensitive UHPLC-DAD strategy was developed and validated. The proposed method allowed determining 49 polyphenols in apple samples and 41 phenolic compounds in apple juice samples in 18 min. Furthermore, six minor flavonoids have been quantified for the first time in apples: phloretin-hexosyl-hexoside, isorhamnetin-3-O-galactoside, isorhamnetin-3-O-glucoside, isorhamnetin-3-O-arabinopyranoside, isorhamnetin-3-O-arabinofuranoside and isorhamnetin-3-O-rhamnoside. The selectivity and reliability of this methodology allowed us to apply it in a successful manner to select the most interesting descendants from a progeny of ‘Meana’ × ‘Florina’ (a cider and a dessert apple, respectively) in terms of their phenolic composition. Descendants X9701-349, 119, 370, 120 and 069 could be proposed for cider production, since they exhibited a higher phenolic content than ‘Meana’ apple juice. Although flavan-3-ol, procyanidin and dihydrochalcone levels were similar to those observed in ‘Meana’ apple, flavonol levels increased slightly. Furthermore, it is worth noting that hydroxycinnamic acid concentration was up to three times higher. This fact could lead to an increase in cider astringency and color, as well as in cider aroma. Thus, this methodology is proposed to be applicable to the determination of the phenolic profile in other fruit and/or plant extracts.

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Acknowledgments

This research was supported by Gobierno Vasco/Eusko Jaularitza (project number IT413-10) and Plan Nacional de I + D+I 2008-2011 (project number RTA2012-00118-C03-03, financed by INIA and co-financed by FEDER funds). María Ramírez-Ambrosi thanks Universidad del País Vasco/Euskal Herriko Unibertsitatea (UPV/EHU) for financial support (Contratación de Doctores Recientes hasta su Integración en Programas de Formación Postdoctoral, Vicerrectorado de Investigación) and Gobierno Vasco/Eusko Jaurlaritza for her Ph.D. Grant. Technical and staff support provided by SGIker (UPV/EHU, MICINN, GV/EJ, ESF) is gratefully acknowledged.

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Authors and Affiliations

  1. Departamento de Química Analítica, Facultad de Ciencia y Tecnología, Universidad del País Vasco/Euskal Herriko Unibertsitatea (UPV/EHU), P.O. Box 644, 48080, Bilbao, Spain

    María Ramírez-Ambrosi, Diana M. López-Márquez, Luis Á. Berrueta & Blanca Gallo

  2. Servicio Central de Análisis, Servicios Generales de Investigación (SGIker), Universidad del País Vasco/Euskal Herriko Unibertsitatea (UPV/EHU), P.O. Box 644, 48080, Bilbao, Spain

    Beatriz Abad-García

  3. Servicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA), Apdo. 13, 33300, Villaviciosa, Asturias, Spain

    Enrique Dapena

Authors
  1. María Ramírez-Ambrosi
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  2. Diana M. López-Márquez
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  3. Beatriz Abad-García
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  4. Enrique Dapena
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  5. Luis Á. Berrueta
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  6. Blanca Gallo
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Corresponding author

Correspondence to Blanca Gallo.

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Electronic supplementary material

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217_2015_2502_MOESM1_ESM.xlsx

Supplementary material 1 The parameters of the regression equations for the polyphenols used for quantification purpose (XLSX 12 kb)

217_2015_2502_MOESM2_ESM.xlsx

Supplementary material 2 A detailed description of the phenolic profile of the 22 apple (data expressed as mg/kg DW ± standard error, n = 3) (XLSX 27 kb)

217_2015_2502_MOESM3_ESM.xlsx

Supplementary material 3 A detailed description of the phenolic profile of the 22 apple juice samples (data expressed as mg/L ±standard error, n = 3) (XLSX 1213 kb)

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Ramírez-Ambrosi, M., López-Márquez, D.M., Abad-García, B. et al. Comparative study of phenolic profile of fruit and juice samples of a progeny of ‘Meana’ × ‘Florina’ from an Asturian cider apple breeding program. Eur Food Res Technol 241, 769–784 (2015). https://doi.org/10.1007/s00217-015-2502-2

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  • DOI: https://doi.org/10.1007/s00217-015-2502-2

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