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Extraction, sulfonation and anticoagulant activity of xylan from corncob

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Abstract

The aim of this work was to study the anticoagulant activity of sulfonated xylan. The crude corncob xylans were isolated from corncob by alkaline extraction. The water-insoluble xylans were sulfonated using chlorosulfonic acid and dimethylformamide (DMF) to generate the water-soluble sulfonated xylan (WISX-S). The best conditions for the esterification were 1:1 of chlorosulfonic acid to DMF, keep time of 6 h and temperature of 70 °C by the orthogonal analysis. Degree of substitution of esterification product was 1.54. Anticoagulant properties of WISX-S1 and WISX-S2 were compared with normal saline and positive control heparin sodium. In vitro tests demonstrated that WISX-S2 can significantly prolong APTT and TT, but not PT. The results suggest that WISX-S2 may play the key role of anticoagulation through the intrinsic pathway and common pathway. The anticoagulant activity of xylan sulfates may be associated with the degree of substitution and molecular weight within a certain range.

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Acknowledgments

The authors are grateful to the National Natural Science Foundation of China (No. 31171753), Open Project of Key Laboratory of Carbohydrate Chemistry and Biotechnology Ministry of Education (No. KLCCB-KF201202), Nature Science Foundation of Anhui Province (No. 1408085MC52) and Students’ Innovative Training Project of Anhui Province (No. 201210363018) for their supply of financial support and are pleased to acknowledge the contribution of Dr. Zhao for her discussions in conducting the anticoagulant bioassays. We thank for her encouragement and suggestions.

Conflict of interest

The authors declare that they have no conflict of interest.

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This article does not contain any studies with human or animal subjects.

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Authors and Affiliations

  1. College of Biological and Chemical Engineering, Anhui Polytechnic University, Wuhu, 241000, People’s Republic of China

    Weirong Cai, Qiao Chen, Liangliang Xie, Lei Yang & Rufei Zhang

  2. Research Center of Microbial Fermentation Engineering, Wuhu, Anhui Province, People’s Republic of China

    Weirong Cai

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  1. Weirong Cai
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  2. Qiao Chen
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  3. Liangliang Xie
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Correspondence to Weirong Cai.

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Cai, W., Chen, Q., Xie, L. et al. Extraction, sulfonation and anticoagulant activity of xylan from corncob. Eur Food Res Technol 240, 969–975 (2015). https://doi.org/10.1007/s00217-014-2401-y

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  • DOI: https://doi.org/10.1007/s00217-014-2401-y

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