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Preparative separation and purification of two flavanols from Smilax china L. using high-speed counter-current chromatography

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Abstract

Natural products are some of the most important sources of lead compounds for drug discovery. It is an effective way to isolate some lead compounds from complex multiconstituent extracts by combining some advanced isolation techniques with therapeutically relevant bioassays. Smilax china L. exhibits important pharmacological property of anti-chronic pelvic inflammation disease (CPID). With the bioassay guiding in induced CPID rats, high-speed counter-current chromatography (HSCCC) was applied to separate and purify some bioactive compounds from the effective solvent extract of the Smilax china L. with the optimized solvent system of n-hexane–ethyl acetate–methanol–water(1:10:1:10, v/v/v/v). 4 mg epicatechin and 10 mg catechin-[8,7-e]-4 β-(3,4-dihydroxyphenyl)-dihydro-2(3H)-pyranone were successfully obtained from 300 mg of crude sample with purity of 98.5 and 95.6 %, respectively. The anti-inflammation of the separated compounds was also studied by MTT assay. The results showed that HSCCC method was successful for the separation of the flavanols from Smilax china L. and suggested catechin-[8,7-e]-4β-(3,4-dihydroxyphenyl)-dihydro-2(3H)-pyranone be one potential compound for the anti-inflammation.

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Acknowledgments

Financial support from Natural Science Foundation of China (31240045) is gratefully acknowledged.

Conflict of interest

None.

Compliance with Ethics Requirements

Research was conducted in compliance with the statutes and regulations pertaining to animals.

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Authors and Affiliations

  1. School of Pharmaceutical Science, Southern Medical University, Guangzhou, 510515, People’s Republic of China

    YinZhou Fan, WenJun Duan, JinXiang Chen & Yang Xie

  2. Department of Pharmacy, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, People’s Republic of China

    YanQin Luo, LuYao Song & LianBing Hou

Authors
  1. YinZhou Fan
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  2. WenJun Duan
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  3. YanQin Luo
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  4. LuYao Song
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  5. JinXiang Chen
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  6. Yang Xie
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  7. LianBing Hou
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Corresponding authors

Correspondence to Yang Xie or LianBing Hou.

Additional information

Y. Fan and W. Duan have equally contributed to this work.

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Fan, Y., Duan, W., Luo, Y. et al. Preparative separation and purification of two flavanols from Smilax china L. using high-speed counter-current chromatography. Eur Food Res Technol 240, 33–39 (2015). https://doi.org/10.1007/s00217-014-2304-y

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  • DOI: https://doi.org/10.1007/s00217-014-2304-y

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