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Investigation of incurred single- and multi-component model food matrices for determination of food proteins triggering allergy and coeliac disease

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Abstract

For detection and quantification of food proteins triggering hypersensitivity reactions, reliable, validated analytical methods are necessary. At present, the most commonly used methodology for this purpose is ELISA. The lack of incurred reference materials (RM) complicates the fulfilling of method validation. Today, a well identifiable research direction is the development of methods (immunoanalytical, PCR and MS methods) suitable for multi-component analysis. As a consequence of these R+D efforts, the development of multi-component RMs is reasonable. Our work presented here is focusing on the development and investigation of multi-component model food matrices that can be suitable for supporting the validation processes. On the basis of the relative incidence of hypersensitivity reactions, four components—wheat, milk, egg and soy—were selected for our preliminary work. The target proteins were determined in single- and multi-component matrices with different ELISA methods, and the results were compared by statistical evaluation. The main direction of our investigation was to identify the effects of the potential interactions of these proteins on the analytical results, and the influence of food processing was also investigated. The results showed that the presence of multiple allergenic components caused much less uncertainty in the analytical results than analytical and technological steps, such as sample preparation, repetition or even heat treatment. However, probably due to the relatively complex matrices, some discrepancies from the expected outcome could also be observed.

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Acknowledgments

This research is related to the scientific goals of MoniQA Association and the Hungarian national project “Development of quality orientated, harmonised educational and R+D+I strategy and operational model at the Budapest University of Technology and Economics” (ÚMFT TÁMOP-4.2.1/B-09/1/KMR-2010-0002).

Conflict of interest

The authors declare that they have no conflict of interest.

Compliance with Ethics Requirements

This article does not contain any studies with human participants or animals performed by any of the authors. Informed consent was obtained from all individual participants included in the study.

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Authors and Affiliations

  1. Department of Applied Biotechnology and Food Science, Budapest University of Technology and Economics, Szent Gellért tér 4. 1/106, Budapest, 1111, Hungary

    Kitti Török, Vanda Horváth, Ágnes Horváth, Lívia Hajas, Zsuzsanna Bugyi & Sándor Tömösközi

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  1. Kitti Török
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  2. Vanda Horváth
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  3. Ágnes Horváth
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  4. Lívia Hajas
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  5. Zsuzsanna Bugyi
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  6. Sándor Tömösközi
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Correspondence to Kitti Török.

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Török, K., Horváth, V., Horváth, Á. et al. Investigation of incurred single- and multi-component model food matrices for determination of food proteins triggering allergy and coeliac disease. Eur Food Res Technol 239, 923–932 (2014). https://doi.org/10.1007/s00217-014-2289-6

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  • DOI: https://doi.org/10.1007/s00217-014-2289-6

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