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Innovative extraction procedure for obtaining high pure lycopene from tomato

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Abstract

Many researchers have studied the biological effects of carotenoids and the more appropriate procedure for extracting them from vegetable sources. In this work we propose a rapid and low-cost procedure to extract lycopene from tomato in order to by-pass the problems related to the high cost of this molecule. Following this procedure we have obtained over 95% pure all-trans-lycopene checked by DAD-HPLC coupled with mass-spectrometer equipped with APCI source and by UV–Vis spectroscopy. Moreover, in order to evaluate the effectiveness of this procedure, we have assayed the capacity of the extracted lycopene to inhibit proliferation in T-lymphocyte jurkat J32 cells in comparison with authentic standard all-trans-lycopene. On this cellular line both standard lycopene and extracted lycopene tended to be dose-dependent but this latter seems to be more active even at lowest concentration.

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Authors and Affiliations

  1. Dipartimento di Scienze Economico-Estimative e degli Alimenti, Sezione di Chimica Bromatologica, Università di Perugia, via Romana, Perugia, Italy

    Domenico Montesano, Lina Cossignani, Ancilla Bosi, Maria Stella Simonetti & Pietro Damiani

  2. Dipartimento di Medicina Sperimentale e Scienze Biochimiche, Università di Perugia, via del Giochetto, Perugia, Italy

    Francesca Fallarino & Paolo Puccetti

Authors
  1. Domenico Montesano
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  2. Francesca Fallarino
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  3. Lina Cossignani
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  4. Ancilla Bosi
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  5. Maria Stella Simonetti
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  6. Paolo Puccetti
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  7. Pietro Damiani
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Correspondence to Pietro Damiani.

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Montesano, D., Fallarino, F., Cossignani, L. et al. Innovative extraction procedure for obtaining high pure lycopene from tomato. Eur Food Res Technol 226, 327–335 (2008). https://doi.org/10.1007/s00217-006-0541-4

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  • DOI: https://doi.org/10.1007/s00217-006-0541-4

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