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Spectroscopic sensors for in-line bioprocess monitoring in research and pharmaceutical industrial application

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Abstract

The use of spectroscopic sensors for bioprocess monitoring is a powerful tool within the process analytical technology (PAT) initiative of the US Food and Drug Administration. Spectroscopic sensors enable the simultaneous real-time bioprocess monitoring of various critical process parameters including biological, chemical, and physical variables during the entire biotechnological production process. This potential can be realized through the combination of spectroscopic measurements (UV/Vis spectroscopy, IR spectroscopy, fluorescence spectroscopy, and Raman spectroscopy) with multivariate data analysis to obtain relevant process information out of an enormous amount of data. This review summarizes the newest results from science and industry after the establishment of the PAT initiative and gives a critical overview of the most common in-line spectroscopic techniques. Examples are provided of the wide range of possible applications in upstream processing and downstream processing of spectroscopic sensors for real-time monitoring to optimize productivity and ensure product quality in the pharmaceutical industry.

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Authors and Affiliations

  1. Institute of Technical Chemistry, Gottfried Wilhelm Leibniz University of Hannover, Callinstraße 5, 30167, Hannover, Germany

    Jens Claßen, Florian Aupert, Dörte Solle & Thomas Scheper

  2. Department of Chemical Biological Engineering, Colorado State University, 344 Scott Bioengineering, Fort Collins, Colorado, 80523-1370, USA

    Kenneth F. Reardon

Authors
  1. Jens Claßen
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  2. Florian Aupert
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  3. Kenneth F. Reardon
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  4. Dörte Solle
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  5. Thomas Scheper
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Correspondence to Dörte Solle.

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Published in the topical collection Process Analytics in Science and Industry with guest editor Rudolf W. Kessler.

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Claßen, J., Aupert, F., Reardon, K.F. et al. Spectroscopic sensors for in-line bioprocess monitoring in research and pharmaceutical industrial application. Anal Bioanal Chem 409, 651–666 (2017). https://doi.org/10.1007/s00216-016-0068-x

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  • DOI: https://doi.org/10.1007/s00216-016-0068-x

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