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High-resolution monolithic columns—a new tool for effective and quick separation

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Abstract

This work describes a comparison of three types of commercial high-performance liquid chromatography silica monolithic columns with different inner diameters and generations of monolithic sorbent: a “classic” monolithic column, the first generation (Onyx™ monolithic C18, 100 mm × 4.6 mm, Phenomenex); a “narrow” monolithic column for fast separation at lower flow rates (Chromolith® Performance RP-18e, 100 mm × 3 mm, Merck); and a recently introduced “high-resolution” monolithic column, the next generation (Chromolith® HighResolution RP-18e, 100 mm × 4.6 mm, Merck). Separation efficiency (number of theoretical plates, height equivalent to a theoretical plate and van Deemter curves), working pressure, the symmetry factor and resolution were critical aspects of the comparison in the case of the separation of ascorbic acid, paracetamol and caffeine. The separations were performed under isocratic conditions with a mobile phase consisting of 10:90 (v/v) acetonitrile–phosphoric acid (pH 2.80). Detailed comparison of the newest-generation monolithic column (Chromolith® HighResolution) with the previously introduced monolithic sorbents was performed and proved the advantages of the Chromolith® HighResolution column.

Chromatogram of separation using different flow rates (corresponded to optimal separation conditions); 1 0.5 mL min-1; 2 0.6 mL min-1; 3 0.3 mL min-1

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Acknowledgments

The authors express their thanks to Egidijus Machtejevas (Analytical Chromatography Product Management, Merck Millipore, Darmstadt, Germany) for providing HRMCs. This study was supported by the Charles University in Prague (projects SVV2012/265 002 and 17/2012/UNCE) and the Grant Agency of Charles University (no. 34609/2009) and was co-financed by the European Social Fund and the state budget of the Czech Republic (TEAB, project no. CZ.1.07/2.3.00/20.0235).

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Authors and Affiliations

  1. Department of Analytical Chemistry, Faculty of Pharmacy, Charles University, Heyrovského 1203, 500 05, Hradec Králové, Czech Republic

    Hana Sklenářová, Petr Chocholouš, Petra Koblová, Lukáš Zahálka, Dalibor Šatínský, Ludmila Matysová & Petr Solich

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  1. Hana Sklenářová
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  2. Petr Chocholouš
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  3. Petra Koblová
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  4. Lukáš Zahálka
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  5. Dalibor Šatínský
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  6. Ludmila Matysová
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  7. Petr Solich
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Corresponding author

Correspondence to Petr Chocholouš.

Additional information

Published in the topical collection Monolithic Columns in Liquid Phase Separations with guest editor Luis A. Colon.

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Sklenářová, H., Chocholouš, P., Koblová, P. et al. High-resolution monolithic columns—a new tool for effective and quick separation. Anal Bioanal Chem 405, 2255–2263 (2013). https://doi.org/10.1007/s00216-012-6561-y

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  • DOI: https://doi.org/10.1007/s00216-012-6561-y

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