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Position-resolved determination of trace elements in mandibular gnathobases of the Antarctic copepod Calanoides acutus using a multimethod approach

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Abstract

Previous studies have revealed silica formation in the teeth of mandibular gnathobases of copepods while significant amounts of zinc and copper are present, which might improve mechanical stability of the teeth and represent an adaptation to compact food particles. The present study aimed at analysing the distribution and concentration of trace elements in the mandibular gnathobases of females of the Antarctic copepod species Calanoides acutus. Because of the low overall masses of few micrograms per specimen the application of a combination of position-resolved micro-beam techniques was necessary and micro-particle-induced X-ray emission spectrometry and laser ablation inductively coupled plasma mass spectrometry were used to determine Ba, Br, Ca, Cl, Cu, Fe, K, Mg, Na, Ni, P, S, Si and Zn in the samples with μm to sub-μm resolution. Calibration strategies were optimised to fit for the carbonate matrix. The analyses revealed a distinct enrichment of Br, Ca, Fe, K, S, Si and Zn in the teeth of the gnathobases.

Elemental distribution in chitinous material from copepod gnathobases, measured by PIXE.

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Acknowledgments

Jan Michels received financial support from the virtual institute “PlanktonTech” of the Helmholtz Society.

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Author notes

  1. Jan Michels

    Present address: Department of Zoology: Functional Morphology and Biomechanics, Christian-Albrechts-Universität zu Kiel, Institute of Zoology, Olshausenstr. 40, 24098, Kiel, Germany

Authors and Affiliations

  1. Leibniz University Hannover, Institute of Inorganic Chemistry, Callinstr. 9, 30167, Hanover, Germany

    Kai Bechstein, Gregor C. Schwartze & Carla Vogt

  2. Alfred Wegener Institute for Polar and Marine Research, P.O. Box 120161, 27570, Bremerhaven, Germany

    Jan Michels

  3. University of Leipzig, Institute of Experimental Physics II, Linnéstr. 5, 04103, Leipzig, Germany

    Jürgen Vogt

Authors
  1. Kai Bechstein
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  2. Jan Michels
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  4. Gregor C. Schwartze
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  5. Carla Vogt
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Correspondence to Carla Vogt.

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Bechstein, K., Michels, J., Vogt, J. et al. Position-resolved determination of trace elements in mandibular gnathobases of the Antarctic copepod Calanoides acutus using a multimethod approach. Anal Bioanal Chem 399, 501–508 (2011). https://doi.org/10.1007/s00216-010-4373-5

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  • DOI: https://doi.org/10.1007/s00216-010-4373-5

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