Abstract
The analytical suitabilities of three different liquid chromatography–tandem mass spectrometry (LC-MS/MS) systems, (1) triple quadrupole (QqQ), (2) conventional 3D ion trap (IT), and (3) quadrupole–linear IT (QqLIT), to determine trace levels of perfluorinated compounds (PFCs) in fish and shellfish were compared. Sample preparation was performed using alkaline extraction and solid-phase-extraction cleanup. This evaluation was focused on both quantitative (sensitivity, precision, and accuracy) and qualitative (identification capabilities) aspects. In the three instruments, the former facet was evaluated using selected reaction monitoring (SRM), which is the standard mode for quantitative LC-MS/MS analysis. Accuracy was similar in the three systems, with recoveries always over 70 %. Precision was better for the QqLIT and QqQ systems (7–15%) than for the IT system (10–17%). The QqLIT (working in SRM mode) and QqQ systems offered a linear dynamic range of at least 3 orders of magnitude, whereas that of the IT system was 2 orders of magnitude. The QqLIT system achieved at least 20-fold higher sensitivity than the QqQ system, and this was at least tenfold higher sensitivity than for the IT system. In the IT system, identification was based on sensitive full mass range acquisition and MSn fragmentation and in the QqLIT system, it was based on the use of an information-dependent-acquisition scan function, which allows the combination of an SRM or MS full scan acting as the survey scan and an enhanced product ion scan followed by MS3 as the dependent scan in the same analysis. Three instruments were applied to monitor the content in fish and shellfish (anchovies, swordfish, tuna, mussels, and oysters) obtained from Valencia and Barcelona markets (Spain). The eight target PFCs were detected at mean concentrations in the range from 10 ng kg-1 (perfluoro-7-methyloctanoic acid and perfluoro-1-decanesulfonate) to 4,200 ng kg-1 (perfluoropentanoic acid). Furthermore, perfluoroheptanoic and perfluoroundecanoic acids (not covered as target analytes) were identified in some samples.
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References
Giesy JP, Kannan K (2001) Environ Sci Technol 35:1339–1342
Kannan K, Koistinen J, Beckmen K, Evans T, Gorzelany JF, Hansen KJ, Jones PD, Helle E, Nyman M, Giesy JP (2001) Environ Sci Technol 35:1593–1598
Kannan K, Franson JC, Bowerman WW, Hansen KJ, Jones PD, Giesy JP (2001) Environ Sci Technol 35:3065–3070
Fromme H, Tittlemier SA, Völkel W, Wilhelm M, Twardella D (2009) Int J Hyg Environ Health 212:239–270
Taniyasu S, Kannan K, Man KS, Gulkowska A, Sinclair E, Okazawa T, Yamashita N (2005) J Chromatogr A 1093:89–97
Dinglasan MJA, Ye Y, Edwards EA, Mabury SA (2004) Environ Sci Technol 38:2857–2864
Llorca M, Farre M, Pico Y, Barcelo D (2009) J Chromatogr A 1216:7195–7204
Angerer J, Ewers U, Wilhelm M (2007) Int J Hyg Environ Health 210:201–228
van Leeuwen SPJ, Swart CP, van der Veen I, de Boer J (2009) J Chromatogr A 1216:401–409
Quinete N, Wu Q, Zhang T, Yun SH, Moreira I, Kannan K (2009) Chemosphere 77:863–869
Farré M, Pérez S, Kantiani L, Barceló D (2008) Trends Anal Chem 27:991–1007
Colombo I, Wolf W, Thompson RS, Farrar DG, Hoke RA, L'Haridon J (2008) Ecotoxicol Environ Saf 71:749–756
Ji K, Kim Y, Oh S, Ahn B, Jo H, Choi K (2008) Environ Toxicol Chem 27:2159–2168
EFSA (2008) EFSA J 653:1–131
UNEP (2010) Stockholm Convention on Persistent Organic Pollutants (POPs). New POPs SC-4/17: Listing of perfluorooctane sulfonic acid, its salts and perfluorooctane sulfonyl fluoride. United Nations Environment Programme, Geneva, Switzerland
European Parliament and the Council (2006) Off J Eur Union L 372:32–34
Pico Y, Barcelo D (2008) Trends Anal Chem 27:821–835
Malik AK, Blasco C, Pico Y (2010) J Chromatogr A 1217:4018–4040
Villagrasa M (2006) López De Alda M, Barceló D. Anal Bioanal Chem 386:953–972
Jahnke A, Berger U (2009) J Chromatogr A 1216:410–421
Voogt PD, López M (2006) Trends Anal Chem 25:326–342
Pico Y, Farre M, Llorca M, Barcelo D (2010) Crit Rev Food Sci Nutr (in press)
Emmett EA, Shofer FS, Zhang H, Freeman D, Desai C, Shaw LM (2006) J Occup Environ Med 48:759–770
Moriwaki H (2005) Curr Org Chem 9:849–857
Cheng X, Klaassen CD (2008) Toxicol Sci 106:37–45
Calafat AM, Ye X, Silva MJ, Kuklenyik Z, Needham LL (2006) Int J Androl 29:166–171
Berger U, Haukas M (2005) J Chromatogr A 1081:210–217
Llorca M, Farre M, Pico Y, Lopez-Teijon M, Alvarez JG, Barcelo D (2010) Environ Int (in press). doi:10.1016/j.envint.2010.04.016
Washington JW, Henderson WM, Ellington JJ, Jenkins TM, Evans JJ (2008) J Chromatogr A 1181:21–32
Ye X, Strynar MJ, Nakayama SF, Varns J, Helfant L, Lazorchak J, Lindstrom AB (2008) Environ Pollut 156:1227–1232
Luque N, Ballesteros-Gomez A, Van Leeuwen S, Rubio S (2010) J Chromatogr A 1217:3774–3782
Commission of the European Communities (2002) Off J Eur Communities L 221:8–36
Yamashita N, Kannan K, Taniyasu S, Horii Y, Okazawa T, Petrick G, Gamo T (2004) Environ Sci Technol 38:5522–5528
Martin JW, Kannan K, Berger U, de Voogt P, Field J, Franklin J, Giesy JP, Harner T, Muir DCG, Scott B, Kaiser M, Järnberg U, Jones KC, Mabury SA, Schroeder H, Simcik M, Sottani C, van Bavel B, Kärrman A, Lindstöm G, Van Leeuwen S (2004) Environ Sci Technol 38:248A–255A
Acknowledgements
This work was supported by the Spanish Ministry of Science and Innovation through the project Consolider-Ingenio 2010 CSD2009-00065 and by the Food Safety Platform Program of the Conselleria de Sanitat of the Generalitat Valenciana through project no. PLAT2009-A-013.
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Llorca, M., Farré, M., Picó, Y. et al. Study of the performance of three LC-MS/MS platforms for analysis of perfluorinated compounds. Anal Bioanal Chem 398, 1145–1159 (2010). https://doi.org/10.1007/s00216-010-3911-5
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DOI: https://doi.org/10.1007/s00216-010-3911-5