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A new label-free amperometric immunosenor for rubella vaccine

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Abstract

A novel label-free amperometric immunosensor for the detection of rubella vaccine was developed by immobilizing anti-rubella serum on bilayer nano-Au/polymerized o-phenylenediamine film with electrodeposited Prussian Blue (PB) as an electrode transfer mediator on the platinum electrode. The redox reactions of PB as a probe on the platinum surface were blocked due to the binding of the antibody to the antigen, which was investigated by cyclic voltammetry. Therefore, the interaction of the antibody with various concentrations of antigen could be detected by measurements of amperometric response in PBS, and the amperometric response on the surface of the modified electrode was inversely proportional to the concentration of rubella vaccine in the sample. The immunosensor showed a specific response to rubella vaccine in the range 8.1×10−8–8.0×10−6 lgCCID50/ml (cell culture infectious dose) and a detection limit of 4.010−8 lgCCID50/ml at a signal-to-noise ratio of 3. To summarize, the present work provides a low-cost, fast response time, highly sensitive and easy-to-prepare method for the determination of antigen in biological products.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (29705001), the Chinese Education Ministry Foundation for excellent young teachers and the Natural Science Foundation of Chongqing City, China.

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Authors and Affiliations

  1. Chongqing Key Laboratory of Analytical Chemistry, College of Chemistry and Chemical Engineering, Southwest China Normal University, Chongqing, 400715, China

    Lingyan Zhang, Ruo Yuan, Yaqin Chai, Dianping Tang & Shurui Cao

  2. School of Material Science & Chemical Engineering, Tianjin Polytechnic University, Tianjin, 300160, China

    Xiaoqing Huang

Authors
  1. Lingyan Zhang
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  2. Ruo Yuan
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  3. Xiaoqing Huang
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  4. Yaqin Chai
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Corresponding author

Correspondence to Ruo Yuan.

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Zhang, L., Yuan, R., Huang, X. et al. A new label-free amperometric immunosenor for rubella vaccine. Anal Bioanal Chem 381, 1036–1040 (2005). https://doi.org/10.1007/s00216-004-3021-3

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  • DOI: https://doi.org/10.1007/s00216-004-3021-3

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