Skip to main content
SpringerLink
Log in

Comparison of biosensors based on entrapment of cholesterol oxidase and cholesterol esterase in electropolymerized films of polypyrrole and diaminonaphthalene derivatives for amperometric determination of cholesterol

  • Special Issue Paper
  • Published:
Analytical and Bioanalytical Chemistry Aims and scope Submit manuscript

Abstract

Cholesterol amperometric biosensors constructed with enzymes entrapped in electropolymerized layers of polypyrrole and poly-naphthalene derivative polymers are compared. The biosensors are based on entrapment of cholesterol oxidase and/or cholesterol esterase in monolayer or multilayer films electrochemically synthesised from pyrrole, 1,8-diaminonaphthalene (1,8-DAN), and 1,5-diaminonaphthalene (1,5-DAN) monomers. Seven configurations were assayed and compared, and different analytical properties were obtained depending on the kind of polymer and the arrangement of the layers. The selectivity properties were evaluated for the different monolayer and bilayer configurations proposed as a function of the film permeation factor. All the steps involved in the preparation of the biosensors and determination of cholesterol were carried out in a flow system. Sensitivity and selectivity depend greatly on hydrophobicity, permeability, compactness, thickness, and the kind of the polymer used. In some cases a protective outer layer of non-conducting poly(o-phenylenediamine) polymer improves the analytical characteristics of the biosensor. A comparative study was made of the analytical performance of each of the configurations developed. The biosensors were also applied to the flow-injection determination of cholesterol in a synthetic serum.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.
Fig. 3.

Similar content being viewed by others

References

  1. Ernst ND, Cleeman JI (2002) Curr Opin Lipidol 13:69–73

    Article  CAS  PubMed  Google Scholar 

  2. Foster R, Cassidy J, O'Donoghue E (2000) Electroanalysis 12:716–721

    Article  CAS  Google Scholar 

  3. Brahim S, Narinesingh D, Guiseppi-Elie A (2001) Anal Chim Acta 448:27–36

    Article  CAS  Google Scholar 

  4. Bongiovanni C, Ferri T, Poscia A, Varalli M, Santucci R, Desideri A (2001) Bioelectrochemistry 54:17–22

    Article  CAS  PubMed  Google Scholar 

  5. Nakaminami T, Ito S, Kuwabata S, Yoneyama H (1999) Anal Chem 71:1068–1076

    Article  CAS  PubMed  Google Scholar 

  6. Yao T, Takashima K (1998) Biosens Bioelectron 13:67–73

    Article  CAS  PubMed  Google Scholar 

  7. Ropers M-H, Bilewicz R, Stébé M-J, Hamidi A, Miclo A, Rogalska E (2001) Phys Chem Chem Phys 3:240–245

    Article  CAS  Google Scholar 

  8. Ram MK, Bertoncello P, Ding H, Paddeu S, Nicolini SC (2001) Biosens Bioelectron 16:849–856

    Article  CAS  PubMed  Google Scholar 

  9. Wallace GG, Smyth M, Zhao H (1999) Trends Anal Chem 18:245–251

    Article  CAS  Google Scholar 

  10. Vidal JC, Garcia E, Castillo JR (1999) Anal Chim Acta 385:213–222

    Article  CAS  Google Scholar 

  11. Farrington AM, Slater JM (1997) Electroanalysis 9:843–847

    CAS  Google Scholar 

  12. Vidal JC, Espuelas J, Garcia-Ruiz E, Castillo JR (2002) Anal Lett 35:837–853

    Article  CAS  Google Scholar 

  13. Murphy LJ (1998) Anal Chem 70:2928–2935

    Article  CAS  Google Scholar 

  14. Jackowska K, Skompska M, Przyluska E (1996) J Electroanal Chem 418:35–39

    Article  CAS  Google Scholar 

  15. Vidal JC, Garcia E, Castillo JR (1999) Sens Actuators B 57:219–226

    Article  Google Scholar 

  16. Vidal JC, Garcia E, Castillo JR (1998) Biosens Bioelectron 13:371–382

    Article  CAS  PubMed  Google Scholar 

  17. Kubiak WW, Wang J (1996) Anal Chim Acta 329:181–189

    Article  CAS  Google Scholar 

  18. Kurganow BI, Lobanov AV, Borisov IA, Reshetilow AN (2001) Anal Chim Acta 427:11–19

    Article  Google Scholar 

  19. Hämmerle M, Schuhmann W (1992) Sens Actuators B 6:106–112

    Article  Google Scholar 

  20. Vernitskaya TV, Efimov ON (1997) Russ Chem Rev 66:443–457

    Article  Google Scholar 

  21. Skompska M, Hillman R (1996) J Chem Soc Faraday Trans 92:4101–4108

    CAS  Google Scholar 

Download references

Acknowledgements

This work was financially supported by the DGI of the Spanish Ministry of Science and Technology, in the framework of the Project BQU2002/02240.

Author information

Authors and Affiliations

  1. Analytical Chemistry Department, Faculty of Science, University of Zaragoza, Plaza San Francisco s/n, 50009, Zaragoza, Spain

    J. C. Vidal, E. Garcia-Ruiz, J. Espuelas, T. Aramendia & J. R. Castillo

Authors
  1. J. C. Vidal
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. Garcia-Ruiz
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. Espuelas
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. T. Aramendia
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. J. R. Castillo
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to J. C. Vidal.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Vidal, J.C., Garcia-Ruiz, E., Espuelas, J. et al. Comparison of biosensors based on entrapment of cholesterol oxidase and cholesterol esterase in electropolymerized films of polypyrrole and diaminonaphthalene derivatives for amperometric determination of cholesterol. Anal Bioanal Chem 377, 273–280 (2003). https://doi.org/10.1007/s00216-003-2120-x

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00216-003-2120-x

Keywords

Search

Navigation