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Problems associated with the use of commercial and non-commercial antibodies against the histamine H4 receptor

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References

  • Allard B, Priam F, Deshayes F, Ducancel F, Boquet D, Wijkhuisen A, Couraud JY (2011) Electroporation-aided DNA immunization generates polyclonal antibodies against the native conformation of human endothelin B receptor. DNA Cell Biol 30:727–737

    Article  PubMed  CAS  Google Scholar 

  • Alousi AA, Jasper JR, Insel PA, Motulsky HJ (1991) Stoichiometry of receptor-Gs–adenylate cyclase interactions. FASEB J 5:2300–2303

    PubMed  CAS  Google Scholar 

  • Bäumer W, Wendorff S, Gutzmer R, Werfel T, Dijkstra D, Chazot P, Stark H, Kietzmann M (2008) Histamine H4 receptors modulate dendritic cell migration through skin—immunomodulatory role of histamine. Allergy 63:1387–1394

    Article  PubMed  Google Scholar 

  • Beermann S, Seifert R, Neumann D (2011) Commercially available antibodies against human and murine histamine H4-receptor lack specificity. Naunyn Schmiedeberg's Arch Pharmacol. doi:10.1007/s00210-011-0700-4

  • Beermann S, Glage S, Jonigk D, Seifert R, Neumann D (2012) Opposite effects of mepyramine on JNJ 7777120-induced amelioration of experimentally induced asthma in mice in sensitization and provocation. PLoS One 7:e30285

    Article  PubMed  CAS  Google Scholar 

  • Bodei S, Arrighi N, Spano P, Sigala S (2009) Should we be cautious on the use of commercially available antibodies to dopamine receptors? Naunyn Schmiedeberg's Arch Pharmacol 379:413–415

    Article  CAS  Google Scholar 

  • Cernecka H, Ochodnicky P, Lamers HL, Michel MC (2012) Specificity evaluation of antibodies against human β3-adrenoceptors. Naunyn Schmiedeberg’s Arch Pharmacol (in press)

  • Connelly WM, Shenton FC, Lethbridge N, Leurs R, Waldvogel HJ, Faull RL, Lees G, Chazot PL (2009) The histamine H4 receptor is functionally expressed on neurons in the mammalian CNS. Br J Pharmacol 157:55–63

    Article  PubMed  CAS  Google Scholar 

  • Defer N, Best-Belpomme M, Hanoune J (2000) Tissue specificity and physiological relevance of various isoforms of adenylyl cyclases. Am J Ren Physiol 279:F400–F416

    CAS  Google Scholar 

  • Dijkstra D, Stark H, Chazot PL, Shenton FC, Leurs R, Werfel T, Gutzmer R (2008) Human inflammatory dendritic epidermal cells express a functional histamine H4 receptor. J Invest Dermatol 128:1696–1703

    Article  PubMed  CAS  Google Scholar 

  • Dunford P, O'Donnell N, Riley J, Williams K, Karlsson L, Thurmond R (2006) The histamine H4 receptor mediates allergic airway inflammation by regulating the activation of CD4+ T cells. J Immunol 176:7062–7070

    PubMed  CAS  Google Scholar 

  • Grube D (1980) Immunoreactivities of gastrin (G−) cells. II. Non-specific binding of immunoglobulins to G-cells by ionic interactions. Histochemistry 66:149–167

    Article  PubMed  CAS  Google Scholar 

  • Gschwandtner M, Rossbach K, Dijkstra D, Bäumer W, Kietzmann M, Stark H, Werfel T, Gutzmer R (2010) Murine and human Langerhans cells express a functional histamine H4 receptor: modulation of cell migration and function. Allergy 65:840–849

    Article  PubMed  CAS  Google Scholar 

  • Gutzmer R, Werfel T, Bäumer W, Kietzmann M, Chazot PL, Leurs R (2012) Well characterized antihistamine 4 receptor antibodies contribute to current knowledge of the expression and biology of the human and murine histamine 4 receptor. Naunyn Schmiedeberg’s Arch Pharmacol. doi:10.1007/s00210-012-0744-0

  • Haga K, Kruse AC, Asada H, Yurugi-Kobayashi T, Shiroishi M, Zhang C, Weis WI, Okada T, Kobilka BK, Haga T, Kobayashi T (2012) Structure of the human M2 muscarinic acetylcholine receptor bound to an antagonist. Nature 482:547–551

    Article  PubMed  CAS  Google Scholar 

  • Hamdani N, van der Velden J (2009) Lack of specificity of antibodies directed against human β-adrenergic receptors. Naunyn Schmiedeberg’s Arch Pharmacol 379:403–407

    Article  CAS  Google Scholar 

  • Hofstra CL, Desai PJ, Thurmond RL, Fung-Leung WP (2003) Histamine H4 receptor mediates chemotaxis and calcium mobilization of mast cells. J Pharmacol Exp Ther 305:1212–1221

    Article  PubMed  CAS  Google Scholar 

  • Jensen BC, Swigart PM, Simpson PC (2009) Ten commercial antibodies for α-1-adrenergic receptor subtypes are nonspecific. Naunyn Schmiedeberg’s Arch Pharmacol 379:409–412

    Article  CAS  Google Scholar 

  • Jositsch G, Papadakis T, Haberberger RV, Wolff M, Wess J, Kummer W (2009) Suitability of muscarinic acetylcholin receptor antibodies for immunohistochemistry evaluated on tissue sections of receptor gene-deficient mice. Naunyn Schmiedeberg’s Arch Pharmacol 379:389–395

    Article  CAS  Google Scholar 

  • Kobilka BK, Deupi X (2007) Conformational complexity of G-protein-coupled receptors. Trends Pharmacol Sci 28:397–406

    Article  PubMed  CAS  Google Scholar 

  • Lethbridge NL, Chazot PL (2010) Immunological identification of the mouse H4 histamine receptor on spinal cord motor neurons using a novel anti-mouse H4R antibody. Inflamm Res 59(Suppl 2):S197–S198

    Article  PubMed  CAS  Google Scholar 

  • Michel MC, Wieland T, Tsujimoto G (2009) How reliable are G-protein-coupled receptor antibodies? Naunyn Schmiedeberg’s Arch Pharmacol 379:385–388

    Article  CAS  Google Scholar 

  • Nakamura T, Itadani H, Hidaka Y, Ohta M, Tanaka K (2000) Molecular cloning and characterization of a new human histamine receptor, HH4R. Biochem Biophys Res Commun 279:615–620

    Article  PubMed  CAS  Google Scholar 

  • Pradidarcheep W, Stallen J, Labruyère WT, Dabhoiwala NF, Michel MC, Lamers WH (2009) Lack of specificity of commercially available antisera against muscarinergic and adrenergic receptors. Naunyn Schmiedeberg’s Arch Pharmacol 379:397–402

    Article  CAS  Google Scholar 

  • Reher T, Neumann D, Buschauer A, Seifert R (2012) Incomplete activation of human eosinophils via the histamine H4-receptor: evidence for ligand-specific receptor conformations. Biochem Pharmacol. doi:10.1016/j.bcp. 2012.04.004

  • Schneider EH, Schnell D, Papa D, Seifert R (2009) High constitutive activity and a G-protein-independent high-affinity state of the human histamine H4-receptor. Biochemistry 48:1424–1438

    Article  PubMed  CAS  Google Scholar 

  • Schnell D, Brunskole I, Ladova K, Schneider EH, Igel P, Dove S, Buschauer A, Seifert R (2011) Expression and functional properties of canine, rat, and murine histamine H4 receptors in Sf9 insect cells. Naunyn Schmiedeberg’s Arch Pharmacol 383:457–470

    Article  CAS  Google Scholar 

  • Seifert R, Lee TW, Lam VT, Kobilka BK (1998) Reconstitution of β2-adrenoceptor-GTP-binding-protein interaction in Sf9 cells—high coupling efficiency in a β2-adrenoceptor-Gsα fusion protein. Eur J Biochem 255:369–382

    Article  PubMed  CAS  Google Scholar 

  • van Rijn RM, Chazot PL, Shenton FC, Sansuk K, Bakker RA, Leurs R (2006) Oligomerization of recombinant and endogenously expressed human histamine H4 receptors. Mol Pharmacol 70:604–615

    Article  PubMed  Google Scholar 

  • Wenzel-Seifert K, Arthur JM, Liu HY, Seifert R (1999) Quantitative analysis of formyl peptide receptor coupling to Giα1, Giα2, and Giα3. J Biol Chem 274:33259–33266

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

This work was supported by grants of the Deutsche Forschungsgemeinschaft (SFB 587, GRK 760) and by the EU COST action BM 0806.

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Authors and Affiliations

  1. Institute for Pharmacology, Hannover Medical School, Hannover, Germany

    Detlef Neumann, Silke Beermann & Roland Seifert

  2. Institute for Pathology, Hannover Medical School, Hannover, Germany

    Lavinia Mägel & Danny Jonigk

  3. Institute for Immunology, University Regensburg, Regensburg, Germany

    Dorothea Weber-Steffens & Daniela Männel

Authors
  1. Detlef Neumann
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  2. Silke Beermann
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  3. Lavinia Mägel
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  4. Danny Jonigk
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  5. Dorothea Weber-Steffens
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  6. Daniela Männel
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  7. Roland Seifert
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Correspondence to Detlef Neumann.

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Neumann, D., Beermann, S., Mägel, L. et al. Problems associated with the use of commercial and non-commercial antibodies against the histamine H4 receptor. Naunyn-Schmiedeberg's Arch Pharmacol 385, 855–860 (2012). https://doi.org/10.1007/s00210-012-0766-7

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  • DOI: https://doi.org/10.1007/s00210-012-0766-7

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