Abstract
The Fusarium head blight of grain cereals is a significant disease worldwide. In Argentina, high levels of contamination with Fusarium proliferatum have been found in crops. Many strains of the Pseudomonas genus antagonize the growth of fungi by different mechanisms, such as the production of antibiotics, siderophores, volatiles, and extracellular enzymes. In this work, we have designed a new system for studying the growth inhibition of F. proliferatum—namely by volatile compounds produced by Pseudomonas fluorescens MGR12. In both rich and minimal media, the bacterium released volatiles that negatively affected the mycelial growth of that phytopathogenic fungus. These bacterial compounds were analyzed by gas chromatography–mass spectrometry, but only a few could be identified by comparing their mass spectra with the libraries of the National Institutes of Standards and Technology MS search.
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Abbreviations
- VOCs:
-
Volatile organic compounds
- PDA:
-
Potato dextrose agar medium
- TSB:
-
Trypticase soya broth
- GMM:
-
Glucose minimal medium
- NA:
-
Nutrient agar medium
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Acknowledgments
The authors thank the laboratory of Mycology of the Universidad Nacional de Río Cuarto for providing strain F. proliferatum RC 479. We are also grateful to M. Palacio for helping with GC-MS analysis and D.F. Haggerty, a retired career investigator and native English speaker, for editing the final version of the manuscript. S. Fischer and E. Jofré are members of the Scientific Researcher Career-CONICET (National Council of Technological Researchs). P. Cordero has a doctoral fellowship from CONICET-Ministerio de Ciencia y Tecnología de Córdoba. This research was supported by SECYT of the Universidad Nacional de Río Cuarto, PIP-CONICET and Ministerio de Ciencia y Tecnología de Córdoba.
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Communicated by Erko Stackebrandt.
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Cordero, P., Príncipe, A., Jofré, E. et al. Inhibition of the phytopathogenic fungus Fusarium proliferatum by volatile compounds produced by Pseudomonas . Arch Microbiol 196, 803–809 (2014). https://doi.org/10.1007/s00203-014-1019-6
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DOI: https://doi.org/10.1007/s00203-014-1019-6